Species spectrum of nontuberculous mycobacteria isolated from suspected tuberculosis patients, identification by multi locus sequence analysis

Species distribution of Non tuberculosis mycobacteria isolates from North of Iran

“…Both methods indicated that 55.55% of identified environmental species were M. fortuitum. Comparing species abundance in this study is in agreement with the previous findings in Iran [18]. In Korea and U.S.A and in many parts of the world, Mycobacterium avium Complex (MAC) is the most common cause of infection among NTM [19,20].…”

“…The digested fragments separated on 3% agarose gel and RFLP patterns were analyzed according to fragment sizes. 17 Gorgan city Runyon IV + + + NM 18 Gorgan Using PRA, we were able to definitively identify 7 out of 8 isolates, since they produced a PRA pattern identical to those of the reference type established species according to algorithm described by Telenti et al [7]. …”

Comment on “Mycobacterium neoaurum line-related bacteremia with pulmonary involvement: Case report and review of literature’’

“…These group of bacteria can contaminated the hospital equipment such as catheter, ventilator, abdominal shunts or other sterile instrument which can cause of opportunistic infection in and cause of immune-compromised patients and even healthy individuals [2,3]. According to the American Thoracic Society (ATS) guideline, identification and differentiation of clinically isolates of Non-tuberculosis mycobacterium is essential for identification of emerging NTM species, final diagnosis, patient management, appropriate identification and epidemiological investigations [4]. …”

“…Identification of NTM using conventional test including acid-fast staining, growth rate, pigment production, biochemical features and metabolization of carbohydrates are time-consuming, labor-intensive, require a skillful microbiologist and unable to discriminate between species of mycobacteria [4]; but molecular methods such as direct sequence analysis of the housekeeping genes including 16S rRNA, rpoB , hsp65 , 16S–23S rRNA internal transcribed spacer (ITS) and gyrB is inexpensive, rapid, reliable method for accurate identification and differentiation of Mycobacterium spp. [4,5].…”

Comment on Mycobacterium Chelonae Infection of the Buttocks Secondary to Lipofilling: A Case Report and Review of the Literature

“…According to the American Thoracic Society (ATS) advice, clinically isolated NTM should be detected at the species level to determine the clinical implications, infection control, epidemiological study, and patient administration. NTM were identified using phenotypic tests (such as growth rate, macroscopic morphological features, growth at different temperatures, biochemical tests including Tween 80 hydrolysis, nitrate reduction, arylsulfatase, urease production, tellurite reduction, salt tolerance and semi-quantitative catalase production) and popular molecular methods including sequencing and PCR restriction fragment length polymorphism using 16S rRNA, hsp65, rpoB and 16S-23S rRNA internal transcribed spacer (ITS) [3].…”