Species specificity of transferein binding, endocytosis and iron internalization by cultured chick myogenic cells

Sorokin, Lydia; Morgan, Evan H.

doi:10.1007/bf00692564

Cited by 11 publications

(4 citation statements)

References 51 publications

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“…A principal component of animal sera is transferrin, an iron-binding glycoprotein present at micromolar concentrations in animal sera and a key component in many serum-free supplements, such as B-27 ( Brewer et al., 1993 , Ponka, 1999 ). A species specificity for the cellular uptake of Fe 2+ -transferrin has been reported between avian and mammalian transferrins ( Shimo-Oka et al., 1986 , Sorokin and Morgan, 1988 ). To address whether avian transferrin could replace chicken serum, PGCs were cultured in a medium lacking chicken serum and containing chicken transferrin (ovotransferrin [OT]) or human transferrin.…”

Section: Resultsmentioning

confidence: 96%

FGF, Insulin, and SMAD Signaling Cooperate for Avian Primordial Germ Cell Self-Renewal

et al. 2015

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SummaryPrecise self-renewal of the germ cell lineage is fundamental to fertility and reproductive success. The early precursors for the germ lineage, primordial germ cells (PGCs), survive and proliferate in several embryonic locations during their migration to the embryonic gonad. By elucidating the active signaling pathways in migratory PGCs in vivo, we were able to create culture conditions that recapitulate this embryonic germ cell environment. In defined medium conditions without feeder cells, the growth factors FGF2, insulin, and Activin A, signaling through their cognate-signaling pathways, were sufficient for self-renewal of germline-competent PGCs. Forced expression of constitutively active MEK1, AKT, and SMAD3 proteins could replace their respective upstream growth factors. Unexpectedly, we found that BMP4 could replace Activin A in non-clonal growth conditions. These defined medium conditions identify the key molecular pathways required for PGC self-renewal and will facilitate efforts in biobanking of chicken genetic resources and genome editing.

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Section: Resultsmentioning

confidence: 96%

FGF, Insulin, and SMAD Signaling Cooperate for Avian Primordial Germ Cell Self-Renewal

et al. 2015

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“…The method described here may be useful to determine phylogenetic relationships using a simple physio-logical approach, as already described e.g. for the vertebrate transferrin endocytosis process (Sorokin and Morgan, 1988).…”

Section: Melanogaster and C Capitata)mentioning

confidence: 99%

Conservation of Hexamerin Endocytosis in Diptera

Burmester

Scheller

1997

European Journal of Biochemistry

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In cyclorrhaphan Diptera at least two different types of haemolymph proteins exist which belong to the class of hexamerins. In the last larval instar of Calliphora vicina, the highly aromatic hexamerin, arylphorin, and the second hexamerin, PII, make up about 90% of haeniolymph proteins. Both of these proteins are selectively taken up by the fat body cells at the end of larval life and share a common membrane-bound receptor. In addition, hexamerins and possible hexamerin receptors of Calliphora vicina, Calliphora vomitoria, Drosophila melanogaster, Ceratitis capitata, Sarcophaga bullata, Musca domestica and Protophormia terraenovae were investigated. Uptake of arylphorin by the larval fat bodies of Calliphoru vicina as well asarylphorin-receptor binding can be competed in vitro by haemolymph from other Diptera. Therefore, hexamerin-receptor binding must be conserved among related cyclorrhaphan Diptera and between different types of hexamerins within a species. As the degree of competition is in good agreement with the presumed phylogenetic distances between these species, the method described here provides a simple tool to estimate evolutionary distances.

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“…Small extracellular vesicles (sEV) are a heterogeneous group of cells-derived membrane vesicles that consist of proteins, lipids, and coding or noncoding RNA molecules derived from the cells of origin. 1,2 A lot of studies have shown that many proteins [3][4][5] and microRNAs [6][7][8][9] are species-specific. Even for some conserved miRNAs, their targeting in different species will be different, like let-7 miRNA family.…”

Section: Introductionmentioning

confidence: 99%

<p>Comparison of the Therapeutic Effect of Allogeneic and Xenogeneic Small Extracellular Vesicles in Soft Tissue Repair</p>

Dong

Wu²,

Zhang

et al. 2020

IJN

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Small extracellular vesicles (sEV) are a heterogeneous group of vesicles that consist of proteins, lipids and miRNA molecules derived from the cell of origin. Although xenogeneic sEV have been applied for soft tissue regeneration successfully, the regeneration effect of allogeneic and xenogeneic sEV has not been compared systematically. Methods: Our previous study has shown that sEV derived from rat adipose tissue successfully induced neoadipose regeneration. In this study, sEV were isolated from rat adipose tissue (r-sEV-AT) and porcine adipose tissue (p-sEV-AT), the morphology, size distribution and marker proteins expression of r-sEV-AT and p-sEV-AT were characterized. Besides, the sEV/AT ratio was evaluated and compared between r-sEV-AT and p-sEV-AT. Rat adipose-derived stromal/ stem cells (rASCs) and rat aorta endothelial cells (rECs) were adopted to test the cellular response to allogeneic and xenogeneic sEV-AT. The effects of allogeneic and xenogeneic sEV-AT on host cells migration and neoadipose formation were evaluated in a subcutaneous customdesigned model. A full-thickness skin wound healing model was used to further compare the ability of allogeneic and xenogeneic sEV-AT in inducing complex soft tissue regeneration. Results: p-sEV-AT showed similar morphology and size distribution to r-sEV-AT. Marker proteins of sEV were detected in both r-sEV-AT and p-sEV-AT. The sEV/AT ratio of porcine was slightly higher than that of rat. The effects of r-sEV-AT and p-sEV-AT on the differentiation of rASCs and rECs showed no significant difference. When allogeneic and xenogeneic sEV-AT were subcutaneously implanted into the back of SD rats, the host cells chemotactic infiltration was observed in 1 week and neoadipose tissue formation was induced in 8 weeks; no significant difference was observed between allogeneic and xenogeneic sEV-AT. For complex soft tissue regeneration, both allogeneic and xenogeneic sEV-AT significantly promoted wound re-epithelialization, granulation tissue formation and hair follicle regeneration and then accelerated skin wound healing. Conclusion: Our results demonstrated that sEV derived from the same tissues of different species might be loaded with similar therapeutic substance benefitting tissue repair and regeneration, and paved the way for future research aimed at xenogeneic sEV application.

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Species specificity of transferein binding, endocytosis and iron internalization by cultured chick myogenic cells

Cited by 11 publications

References 51 publications

FGF, Insulin, and SMAD Signaling Cooperate for Avian Primordial Germ Cell Self-Renewal

FGF, Insulin, and SMAD Signaling Cooperate for Avian Primordial Germ Cell Self-Renewal

Conservation of Hexamerin Endocytosis in Diptera

<p>Comparison of the Therapeutic Effect of Allogeneic and Xenogeneic Small Extracellular Vesicles in Soft Tissue Repair</p>

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