Species-specific responses of marine bacteria to environmental perturbation

Peña-Montenegro, Tito D; Kleindienst, Sara; Allen, Andrew E; Eren, A Murat; McCrow, John P; Sánchez-Calderón, Juan D; Arnold, Jonathan; Joye, Samantha B

doi:10.1038/s43705-023-00310-z

Cited by 4 publications

(2 citation statements)

References 93 publications

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“…Glutamate and glutamine metabolism were highly expressed relative to other forms of amino acid, peptide and protein metabolism, and strongly expressed across various taxa, including Alteromonas, Pseudoalteromonas, Marinomonas, Vibrio, and Synechococcus, indicating their role in nitrogen assimilation and biosynthesis of essential cellular components (Walker and van der Donk, 2016). Species-specific responses to oil and Corexit exposure have been observed in relation to glutamate and glutamine metabolism (Peña-Montenegro et al, 2023).…”

Section: Nutrient Cycling In the Presence Of Light Oil And Corexitmentioning

confidence: 98%

“…The presence of Corexit led to an increase in the expression of TonB-dependent transport proteins. These proteins are known to be associated with either oil exposure (Knapik et al, 2020) or the application of Corexit (Peña-Montenegro et al, 2023). Starvationrelated proteins were expressed in Corexit-containing treatments, indicating potential stress in nutrient-limited waters.…”

Section: Corexit Unveiled: Evidence Of Enhanced Oxidative Stressmentioning

confidence: 99%

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Clarifying the murk: unveiling bacterial dynamics in response to crude oil pollution, Corexit-dispersant, and natural sunlight in the Gulf of Mexico

Matallana-Surget,

Nigro,

Waidner

et al. 2024

Front. Mar. Sci.

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The 2010 Deepwater Horizon (DwH) Oil spill released an enormous volume of oil into the Gulf of Mexico (GoM), prompting the widespread use of chemical dispersants like Corexit® EC9500A. The ecological consequences of this treatment, especially when combined with natural factors such as sunlight, remain unexplored in the context of marine bacterial communities’ dynamics. To address this knowledge gap, our study employed a unique metaproteomic approach, investigating the combined effects of sunlight, crude Macondo surrogate oil, and Corexit on GoM microbiome across different mesocosms. Exposure to oil and/or Corexit caused a marked change in community composition, with a decrease in taxonomic diversity relative to controls in only 24 hours. Hydrocarbon (HC) degraders, particularly those more tolerant to Corexit and phototoxic properties of crude oil and/or Corexit, proliferated at the expense of more sensitive taxa. Solar radiation exacerbated these effects in most taxa. We demonstrated that sunlight increased the dispersant’s toxicity, impacting on community structure and functioning. These functional changes were primarily directed by oxidative stress with upregulated proteins and enzymes involved in protein turnover, general stress response, DNA replication and repair, chromosome condensation, and cell division. These factors were more abundant in chemically treated conditions, especially in the presence of Corexit compared to controls. Oil treatment significantly enhanced the relative abundance of Alteromonas, an oil-degrading Gammaproteobacteria. In combined oil-Corexit treatments, the majority of identified protein functions were assigned to Alteromonas, with strongly expressed proteins involved in membrane transport, motility, carbon and amino acid metabolism and cellular defense mechanisms. Marinomonas, one of the most active genera in dark conditions, was absent from the light treatment. Numerous metabolic pathways and HC-degrading genes provided insights into bacterial community adaptation to oil spills. Key enzymes of the glyoxylate bypass, enriched in contaminant-containing treatments, were predominantly associated with Rhodobacterales and Alteromonadales. Several proteins related to outer membrane transport, photosynthesis, and nutrient metabolisms were characterized, allowing predictions of the various treatments on biogeochemical cycles. The study also presents novel perspectives for future oil spill clean-up processes.

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Section: Nutrient Cycling In the Presence Of Light Oil And Corexitmentioning

confidence: 98%

Section: Corexit Unveiled: Evidence Of Enhanced Oxidative Stressmentioning

confidence: 99%

Clarifying the murk: unveiling bacterial dynamics in response to crude oil pollution, Corexit-dispersant, and natural sunlight in the Gulf of Mexico

Matallana-Surget,

Nigro,

Waidner

et al. 2024

Front. Mar. Sci.

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Advancements in bacterial chemotaxis: Utilizing the navigational intelligence of bacteria and its practical applications

Xu,

Ali,

Afzal

et al. 2024

Science of The Total Environment

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Genetic redundancy in the naphthalene-degradation pathway of Cycloclasticus pugetii strain PS-1 enables response to varying substrate concentrations

Vogel,

Thompson,

Straub

et al. 2024

FEMS Microbiology Ecology

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Polycyclic aromatic hydrocarbon (PAH) contamination in marine environments range from low-diffusive inputs to high loads. The influence of PAH concentration on the expression of functional genes (e.g., those encoding ring-hydroxylating dioxygenases; RHDs), has been overlooked in PAH biodegradation studies. However, understanding marker-gene expression under different PAH loads can help monitor and predict bioremediation efficiency. Here, we followed the expression (via RNA sequencing) of Cycloclasticus pugetii strain PS-1 in cell suspension experiments under different naphthalene (100 and 30 mg L-1) concentrations. We identified genes encoding previously uncharacterized RHD subunits, termed rhdPS1α and rhdPS1β, that were highly transcribed in response to naphthalene-degradation activity. Additionally, we identified six RHD subunit-encoding genes that responded to naphthalene exposure. In contrast, four RHD subunit genes were PAH-independently expressed and three other RHD subunit genes responded to naphthalene starvation. Cycloclasticus spp. could, therefore, use genetic redundancy in key PAH-degradation genes to react to varying PAH loads. This genetic redundancy may restrict the monitoring of environmental hydrocarbon-degradation activity using single-gene expression. For Cycloclasticus pugetii strain PS-1, however, the newly identified rhdPS1α and rhdPS1β genes might be potential target genes to monitor its environmental naphthalene-degradation activity.

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Species-specific responses of marine bacteria to environmental perturbation

Cited by 4 publications

References 93 publications

Clarifying the murk: unveiling bacterial dynamics in response to crude oil pollution, Corexit-dispersant, and natural sunlight in the Gulf of Mexico

Clarifying the murk: unveiling bacterial dynamics in response to crude oil pollution, Corexit-dispersant, and natural sunlight in the Gulf of Mexico

Advancements in bacterial chemotaxis: Utilizing the navigational intelligence of bacteria and its practical applications

Genetic redundancy in the naphthalene-degradation pathway of Cycloclasticus pugetii strain PS-1 enables response to varying substrate concentrations

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