Species-specific PCR primers for the detection of poorly distinguishable Xanthomonas euvesicatoria

Pecenka, J; Kocanová, Mária; Baránek, Miroslav; Gazdík, Filip; Ragasová, Lucia; Peňázová, Eliška; Čechová, Jana; Beran, Pavel; Eichmeier, Aleš

doi:10.1016/j.cropro.2019.104978

Cited by 9 publications

(3 citation statements)

References 27 publications

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“…euvesicatoria pv. euvesicatoria strains but not in the other three bacterial spot pathogens (Moretti et al, 2009 ; Pečenka et al, 2020 ). Furthermore, the unique gene recG has been used to design primers for a loop‐mediated isothermal amplification assay to rapidly and accurately identify and differentiate X .…”

Section: Detection Isolation and Identification Of The Pathogenmentioning

confidence: 99%

“…in tomato and pepper seeds. Beside the conventional specific/semispecific culture media, serological techniques, DNA fingerprinting methods, and highly specific and sensitive PCR‐based techniques are available for the detection of bacterial spot pathogens (Leite et al, 1995 ; Pečenka et al, 2020 ). Simultaneous occurrence of bacterial spot and bacterial canker (caused by Clavibacter michiganensis ) pathogens in the same tomato field has been recorded (Ansari et al, 2019 ; Osdaghi, Ansari, et al, 2018 ).…”

Section: Detection Isolation and Identification Of The Pathogenmentioning

confidence: 99%

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A centenary for bacterial spot of tomato and pepper

Osdaghi

Jones

Sharma

et al. 2021

Molecular Plant Pathology

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Section: Detection Isolation and Identification Of The Pathogenmentioning

confidence: 99%

Section: Detection Isolation and Identification Of The Pathogenmentioning

confidence: 99%

A centenary for bacterial spot of tomato and pepper

Osdaghi

Jones

Sharma

et al. 2021

Molecular Plant Pathology

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“…gardneri , and X. vesicatoria . Several PCR approaches have been developed to detect and identify the specific strains causing the disease, using primers for rhs family, housekeeping genes and recently, a coding sequence of a zinc-dependent oxidoreductase [ 100 , 101 , 102 , 103 , 104 , 105 ].…”

Section: Molecular Methods In Diagnosis and Detection Of Regulated Xanthomonadsmentioning

confidence: 99%

Trends in Molecular Diagnosis and Diversity Studies for Phytosanitary Regulated Xanthomonas

et al. 2021

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Bacteria in the genus Xanthomonas infect a wide range of crops and wild plants, with most species responsible for plant diseases that have a global economic and environmental impact on the seed, plant, and food trade. Infections by Xanthomonas spp. cause a wide variety of non-specific symptoms, making their identification difficult. The coexistence of phylogenetically close strains, but drastically different in their phenotype, poses an added challenge to diagnosis. Data on future climate change scenarios predict an increase in the severity of epidemics and a geographical expansion of pathogens, increasing pressure on plant health services. In this context, the effectiveness of integrated disease management strategies strongly depends on the availability of rapid, sensitive, and specific diagnostic methods. The accumulation of genomic information in recent years has facilitated the identification of new DNA markers, a cornerstone for the development of more sensitive and specific methods. Nevertheless, the challenges that the taxonomic complexity of this genus represents in terms of diagnosis together with the fact that within the same bacterial species, groups of strains may interact with distinct host species demonstrate that there is still a long way to go. In this review, we describe and discuss the current molecular-based methods for the diagnosis and detection of regulated Xanthomonas, taxonomic and diversity studies in Xanthomonas and genomic approaches for molecular diagnosis.

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