Species-specific PCR for the identification of ovine, porcine and chicken species in meat and bone meal (MBM)

Lahiff, Sinéad; Glennon, M.; O'Brien, Liz; Lyng, James G.; Smith, Terry J.; Maher, Majella; Shilton, N.

doi:10.1006/mcpr.2000.0336

Cited by 135 publications

(79 citation statements)

References 20 publications

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“…1A). Lahiff et al (2001) confirmed the specificity of porcine and ovine/caprine primers against a range The sensitivity of the methods was 1% for bovine and 1.5% for porcine and ovine/caprine bone meal. Matsunga et al (1999) found a detection limit of 0.25 ng of DNA with a multiplex PCR assay aimed at simultaneous PCR amplification of a region of the mitochondrial cytb gene from six species.…”

Section: Introductionsupporting

confidence: 64%

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Short communication. PCR detection of DNA of bovine, ovine-caprine and porcine origin in feed as part of a bovine spongiform encephalopathy control program

Corona¹,

Lleonard²,

Carpio³

et al. 2013

Span. j. agric. res.

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The rapid identification of residues of mammalian materials in animal feedstuffs is important for the effective control of feed as a potential source of transmission of bovine spongiform encephalopathy (BSE). It is included in the monitoring program developed to avoid the appearance of this disease. In the present work a PCR analysis was tested for the detection of mammalian residues (bovine, caprine, ovine and porcine). The DNA extraction method utilised guanidium thiocianate, and primers flanking a conserved region of mitochondrial DNA were synthesized for each species. PCR tests were specific for each species and allowed detection in feedstuffs of levels as low as 1% of bovine DNA and 1.5% of ovine, caprine and porcine DNA. These PCR tests may allow the rapid detection of residues of these species constituting a powerful tool against BSE

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Section: Introductionsupporting

confidence: 64%

“…J01394) ( Table 1). Species-specific primers for the detection of ovine/caprine and porcine DNA were as described by Lahiff et al (2001) (Table 1).…”

Section: Introductionmentioning

confidence: 99%

Short communication. PCR detection of DNA of bovine, ovine-caprine and porcine origin in feed as part of a bovine spongiform encephalopathy control program

Corona¹,

Lleonard²,

Carpio³

et al. 2013

Span. j. agric. res.

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“…Minarovic et al (2010) successfully to identify species using PCR-RFLP with same primer for all species (i.e. Mustela vison (American mink), Mustela putorius furo (Ferret), Sus scrofa domesticus (pig), Oryctolagus cuniculus (Rabbit)), which were designed by Kocher et al (1989). PCR products length did not different for all species, 359 bp, furthermore were cleaved by restriction enzyme AluI.…”

Section: Specific Fragments Amplification Of Cyt β Gene On Dog Catmentioning

confidence: 99%

Species Authentication of Dog, Cat, and Tiger Using Cytochrome β Gene

Irine¹,

Nuraini²,

Sumantri³

2013

Med Pet

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Adulteration of animal food products for economic reason has happened during the last decades. Species identification method development was needed to prevent falsification information. The objective of this research was to study species authentication (dog, cat, and tiger) to ensure animal origin in products using cyt β gene specific marker. DNA extraction and fragment amplification were conducted using phenol-chloroform and multiplex PCR (Polymerase Chain Reaction) method, respectively. This research showed that fragment length of amplification for species tested (dog, cat, and tiger) were 523, 331, 319 bp, respectively. Species specificity was also indicated by high reverse primers homology percentage. Multiplex PCR technique succeed to amplify DNA fragment from species tested, but has a limitation to amplify total DNA composite of mix DNA. Key words: cat, cytochrome β gene, dog, multiplex PCR, tiger ABSTRAKUpaya pemalsuan produk pangan asal ternak dengan alasan ekonomi masih sering terjadi hingga saat ini. Pengembangan metode identifikasi spesies diharapkan dapat melindungi konsumen dari pemalsuan informasi. Penelitian ini bertujuan untuk mempelajari pembuktian spesies anjing, kucing, dan harimau menggunakan marka spesifik berbasis gen sitokrom β (cyt β). Ekstraksi DNA dilakukan dengan metode fenol-kloroform, semetara amplifikasi fragmen DNA menggunakan metode multipleks PCR. Penelitian ini menunjukkan bahwa amplifikasi panjang fragmen pada spesies anjing, kucing, dan harimau adalah 523, 568, dan 319 pb. Selain itu, kespesifikan spesies juga ditunjukkan dengan persentase homologi primer reverse yang tinggi pada masing-masing spesies. Metode multipleks PCR berhasil mengamplifikasi fragmen DNA dari semua spesies yang diuji, namun mempunyai keterbatasan dalam mengamplifikasi gabungan DNA total semua spesies.

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“…On the account, elaboration of new methods for the species identification of MBM are necessary and has prompted numerous studies (4,11,16,18,20,22,27,29,31,33,40). The application of molecular techniques, which tend to be more sensitive and applicable even to high-processed products, has gained an increasing interest.…”

Section: Introductionmentioning

confidence: 99%

“…Several studies have dealt with the application of polymerase chain reaction (PCR) for the detection of bovine tissue in animal feeds (3,27,28,39,41). In many studies (3,5,6,12,14,26,29,30,32), a species-specific PCR has been developed to identify bovine, porcine and avian tissues in meat and bone meal.…”

Section: Introductionmentioning

confidence: 99%

DNA based approaches for the detection and identification of bovine meat and bone meal in feeds

Weiner

Golebiowska

Paprocka

et al. 2013

Bulletin of the Veterinary Institute in Pulawy

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The aim of this paper was to present the results of comparative evaluation of the usefulness of PCR method for the detection and identification of bovine DNA in feeds. In the validation study, the limit of detection for PCR was determined as 0.05% for bovine meat and bone meal (MBM). Among 132 feed samples, bovine DNA was detected in eight (6.06%) samples. In the next stage of the study, sediment and flotate from the investigated samples were examined with PCR. Out of 132 sediment and flotate samples, bovine DNA was detected in eight (6.06%) and nine (6.82%) samples, respectively. On the basis of the results obtained with the use of the PCR, it is possible to state that the molecular biology methods can, at present, be used as supplementary tools for detection and identification of bovine MBM.

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Species-specific PCR for the identification of ovine, porcine and chicken species in meat and bone meal (MBM)

Cited by 135 publications

References 20 publications

Short communication. PCR detection of DNA of bovine, ovine-caprine and porcine origin in feed as part of a bovine spongiform encephalopathy control program

Short communication. PCR detection of DNA of bovine, ovine-caprine and porcine origin in feed as part of a bovine spongiform encephalopathy control program

Species Authentication of Dog, Cat, and Tiger Using Cytochrome β Gene

DNA based approaches for the detection and identification of bovine meat and bone meal in feeds

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