Species-specific PCR-based assays for the detection of Fusarium species and a comparison with the whole seed agar plate method and trichothecene analysis

Demeke, Tigst; Clear, R. M.; Patrick, Susan K.; Gaba, D.

doi:10.1016/j.ijfoodmicro.2004.12.026

Cited by 136 publications

(71 citation statements)

References 29 publications

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“…Whole seed plating appears superior to qualitative PCR-based testing when assessing the risk of mycotoxin contamination because it provides an estimate of the degree of fungal infection in the sample, while a qualitative PCR-based method can only indicate the presence or absence of the target species (Demeke et al 2005). The agar method is also more suitable in detecting viability of the pathogens after thermal treatment (Clear et al 2002) or spraying with a fungicide.…”

Section: Discussionmentioning

confidence: 99%

Real-Time PCR and Agar Plating Method to Predict Fusarium Verticillioides and Fumonisin B1 Content in Nigerian Maize

Adejumo¹,

Hettwer²,

Nutz³

et al. 2009

Journal of Plant Protection Research

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Eighty maize grain samples collected in Nigeria were investigated for fumonisin B 1 (FB 1 ) content and Fusarium verticillioides colonization. F. verticillioides DNA was quantified by species-specific real-time PCR and living propagules of the fungus were counted by agar-plating method. FB 1 was detected in 55 (68.7%) of the total samples (mean: 98.5 µg/kg, range: 10 to 714 µg/kg) at 10 µg/kg detection limit. The mean amount of F. verticillioides DNA determined by real-time PCR was 49.7 µg/kg (range: 10-126.7 µg/kg), while agar plate method showed the presence of F. verticillioides in 45 samples (mean incidence: 21.0%, range: 6.7-60.0%). There was correlation ties between F. verticillioides DNA by real time PCR and fungal colonization by agar plate method (R = 0.71, p = 00001 at 95% confidence level), and means of FB 1 and F. verticillioides DNA in the yellow and white maize were significantly different. Despite the high consumption of maize in Nigeria, the amount of FB 1 ingested by consumers appears to be low. The estimated daily intake of fumonisins was 0.21 µg/kg body weight per day.

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Section: Discussionmentioning

confidence: 99%

Real-Time PCR and Agar Plating Method to Predict Fusarium Verticillioides and Fumonisin B1 Content in Nigerian Maize

Adejumo¹,

Hettwer²,

Nutz³

et al. 2009

Journal of Plant Protection Research

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“…DNA was quantified using a Nanodrop (Thermo, Waltham, MA, USA) and dilutions were performed for PCR optimization. Specific primers obtained from Eurogentec (Lie`ge, Belgium) for species confirmation were used (Demeke et al, 2005) to confirm morphological identification.…”

Section: Molecular Identification Of Fusarium Strainsmentioning

confidence: 99%

Fusarium head blight and associated mycotoxin occurrence on winter wheat in Luxembourg in 2007/2008

Giraud¹,

Pasquali²,

Jarroudi

et al. 2010

Food Additives & Contaminants: Part A

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“…Identification of fungi of the genus Fusarium, based on the morphology of mycelium and macroconidia, is a reliable method but it requires time and necessary skills. The polymerase chain reaction (PCR) technique is one of the most frequently used molecular tools for rapid and sensitive identification of Fusarium species (Niessen et al 2004;Mulé et al 2005;Demeke et al 2005;Jurado et al 2005Jurado et al , 2006.…”

Section: Introductionmentioning

confidence: 99%

Early PCR-based detection of Fusarium culmorum, F. graminearum, F. sporotrichioides and F. poae on stem bases of winter wheat throughout Poland

et al. 2014

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Foot rot and crown rot are fungal diseases of wheat caused by a complex of Fusarium species. They have a huge economic impact mainly due to yield reduction. A survey was conducted to identify four Fusarium species, occurring on wheat stem bases, using speciesspecific PCR assays in samples collected during spring of 2012. The dominant species was F. graminearum, which was identified in above 64 % of samples. F. culmorum was detected in 15.71 %, F. poae in 15.71 % and F. sporotrichioides in 5.71 % wheat fields. Most of the wheat fields in the eastern Poland were infected with at least one or two of Fusarium species, while in central Poland no Fusarium species were identified in most of the fields. The presence of F. graminearum tends to favor the presence of F. culmorum and this effect was visible also for F. poae and F. sporotrichioides. The frequency of F. graminearum and F. culmorum detections were highest where wheat crops were preceded by maize and in the samples from late sown fields. The opposite observation was made for F. poae and F. sporotrichioides, where the number of detections of these species was higher in samples from early sown fields. The number of detected Fusarium species was significantly lower in samples collected from fields protected with autumn herbicide in comparison to unprotected fields. The rate of autumn N fertilization did not affect the number of Fusarium detections.

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Species-specific PCR-based assays for the detection of Fusarium species and a comparison with the whole seed agar plate method and trichothecene analysis

Cited by 136 publications

References 29 publications

Real-Time PCR and Agar Plating Method to Predict Fusarium Verticillioides and Fumonisin B1 Content in Nigerian Maize

Real-Time PCR and Agar Plating Method to Predict Fusarium Verticillioides and Fumonisin B1 Content in Nigerian Maize

Fusarium head blight and associated mycotoxin occurrence on winter wheat in Luxembourg in 2007/2008

Early PCR-based detection of Fusarium culmorum, F. graminearum, F. sporotrichioides and F. poae on stem bases of winter wheat throughout Poland

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