Species‐specific measurement of the second group of <i>Dermatophagoides</i> mite allergens, Der p 2 and Der f 2, using a monoclonal antibody‐based ELISA

Yasueda, Hiroshi; Saito, Akemi; Yanagihara, Yukiyoshi; Akiyama, Kazuo; Takaoka, Masatoshi

doi:10.1111/j.1365-2222.1996.tb00076.x

Cited by 16 publications

(11 citation statements)

References 20 publications

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“…The concentrations of group 2 were surprisingly similar to group 1 [4, 138, 139]even though the group 2 allergens have been reported to be found at about a 5-fold lower concentration in mite extracts and in higher concentration in body extracts than spent mite medium [29, 76]. …”

Section: Group 2 Allergensmentioning

confidence: 99%

Characterization and Immunobiology of House Dust Mite Allergens

Thomas¹,

Smith²,

Hales³

et al. 2002

Int Arch Allergy Immunol

304

254

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The examination of house dust mite extracts has indicated that over 30 different proteins can induce IgE antibody in patients allergic to the house dust mite. There are however dominant specificities especially the group 1 and 2 allergens which can account for much of the allergenicity of extracts. Of the 19 denominated allergens, the major IgE binding has been reported for the group 1, 2, 3, 9, 11, 14 and 15 allergens. The high-molecular-weight group 11, 14 and 15 allergens have only recently been described and although high IgE binding has been anticipated from immunoblotting, there is a need for considerable corroboration. Similarly, the study of the group 3 and 9 serine protease allergens has been incomplete. The group 4, 5, 7 and 8 allergens have shown intermediate IgE binding and the group 10 tropomyosins are of interest because of their potential cross-reactivity with allergen from disparate species. Although the progress with the production of recombinant group 1 allergens has been recent, many of the allergens can be produced as high IgE-binding polypeptides. The tertiary structure of the group 2 allergens has been determined from recombinant proteins and they are an excellent model for the investigation of modified allergens. An unexpected property of the group 1, 2 and 3 allergens has been the high degree of polymorphism found by cDNA analysis. It has however been possible to identify sequences to represent the variation in the natural allergens. The group 7 and 14 allergens show secondary modifications which vary in different extracts creating batch variation. While some estimate of the importance of allergens can be obtained from IgE binding, few analyses of T-cell responses have been made and these regulate both the development of, and the protection from sensitization.

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Section: Group 2 Allergensmentioning

confidence: 99%

Characterization and Immunobiology of House Dust Mite Allergens

Thomas¹,

Smith²,

Hales³

et al. 2002

Int Arch Allergy Immunol

304

254

View full text Add to dashboard Cite

show abstract

“…Previously, the 92-Dp and 92-Df extracts were prepared from HDM bodies and the contents of group 1 and group 2 allergens in 92-Dp and 92-Df were determined. 18,19 For validation of the measurement obtained by this method, group 1 allergen content was also determined using other ELISA kits purchased from Indoor Biotechnologies (Charlottesville, VA, USA) and Nichinichi Pharmaceutical (Mie, Japan).…”

Section: Measurement Of Hdm Group 1 Major Allergensmentioning

confidence: 99%

“…18,19 The group 1 and group 2 allergen contents in the JSA reference HDM extract were measured by sandwich ELISAs using the 92-Dp and/or 92-Df extract as the standard antigen for ELISA ( Table 1) (Table 2). Similar results were obtained using two commercially available ELISA kits.…”

Section: Major Allergen Contents In the Jsa Reference Hdm Extractmentioning

confidence: 99%

“…20,21 Briefly, a sandwich ELISA for Der 2 was performed using rabbit polyclonal antibodies and a mixture of 92-Dp and 92-Df extracts 18,19 as the standard antigen for ELISA.…”

Section: Measurement Of the Total Amount Of Hdm Group 2 Major Allergensmentioning

confidence: 99%

“…18 Briefly, sandwich ELISAs for Der p 1 or Der f 1 were performed using murine monoclonal antibodies and 92-Dp or 92-Df extract, 18,19 respectively, as the standard antigen for ELISA. Previously, the 92-Dp and 92-Df extracts were prepared from HDM bodies and the contents of group 1 and group 2 allergens in 92-Dp and 92-Df were determined.…”

Section: Measurement Of Hdm Group 1 Major Allergensmentioning

confidence: 99%

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