Species-specific interaction of seminal plasma on sperm–neutrophil binding

Alghamdi, Abdorrahman S.; Lovaas, Bethany J.; Bird, Scott; Lamb, G. C.; Rendahl, Aaron; Taube, Patrick C.; Foster, Douglas N.

doi:10.1016/j.anireprosci.2008.10.015

Cited by 82 publications

(87 citation statements)

References 45 publications

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“…In this context, it has been found that leukocytes are the principal source of ROS in seminal plasma and that the main leukocyte population in semen are neutrophils, since it has been shown that their presence at the moment of semen deposition reduces the fertility in different species, including cattle, horses, and pigs (26)(27)(28)(29)(30). Different studies agree with this conclusion, stating that these extracellular traps associated with proteins trap the spermatozoa and hamper their motility (4,16,31). Because the structure of NETs includes DNA, their surface has a negative charge and contains molecules that may mediate bonds with microorganisms, possibly through electrostatic interactions between the cationic components of the NETs and their anionic surfaces (32).…”

Section: Discussionmentioning

confidence: 50%

Leukocytes coincubated with human sperm trigger classic neutrophil extracellular traps formation, reducing sperm motility

Zambrano

Carrau

Gärtner

et al. 2016

Fertility and Sterility

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Section: Discussionmentioning

confidence: 50%

Leukocytes coincubated with human sperm trigger classic neutrophil extracellular traps formation, reducing sperm motility

Zambrano

Carrau

Gärtner

et al. 2016

Fertility and Sterility

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“…Most of them are lost in the vagina, cervix, or uterus by backflow, degradation, and phagocytosis by polymorphonuclear neutrophils (PMNs; Strzemienski 1989). Insemination triggers a massive recruitment of PMNs into the uterine lumen (Mattner 1968, Strzemienski 1989, Alghamdi et al 2009. The mechanism by which neutrophils phagocytize sperm is similar to that for bacteria (Alghamdi & Foster 2005).…”

Section: Introductionmentioning

confidence: 99%

“…The mechanism by which neutrophils phagocytize sperm is similar to that for bacteria (Alghamdi & Foster 2005). Thus, neutrophils either directly phagocytize sperm through cell-cell attachment or entrap them with neutrophil extracellular traps (NETs), structures consisting of neutrophil nuclear DNA and associated proteins, which ensnare sperm and hinder their motility (Alghamdi & Foster 2005, Alghamdi et al 2009). On the other hand, it has been shown that equine seminal plasma (SP) prevents the formation of NETs (Alghamdi & Foster 2005, Alghamdi et al 2009).…”

Section: Introductionmentioning

confidence: 99%

“…Thus, neutrophils either directly phagocytize sperm through cell-cell attachment or entrap them with neutrophil extracellular traps (NETs), structures consisting of neutrophil nuclear DNA and associated proteins, which ensnare sperm and hinder their motility (Alghamdi & Foster 2005, Alghamdi et al 2009). On the other hand, it has been shown that equine seminal plasma (SP) prevents the formation of NETs (Alghamdi & Foster 2005, Alghamdi et al 2009). Moreover, it has been found by Doty et al (2011) that CRISP3 protein in equine SP is the factor that suppresses PMN and sperm binding and regulates sperm degradation.…”

Section: Introductionmentioning

confidence: 99%

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Bovine oviduct epithelial cells downregulate phagocytosis of sperm by neutrophils: prostaglandin E2 as a major physiological regulator

Marey¹,

Liu²,

Kowsar³

et al. 2014

Reproduction

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This study aimed to investigate the presence of polymorphonuclear neutrophils (PMNs) in bovine oviduct fluid under physiological conditions and to determine the possible role of bovine oviduct epithelial cells (BOECs) in the regulation of the phagocytic activity of PMNs for sperm. During the pre-ovulatory stage, PMNs were identified in the bovine oviduct fluid in relatively constant numbers. In our experiments, PMNs were incubated for 4 h with the supernatant of cultured BOECs stimulated for 24 h by LH (10 ng/ml). Phagocytosis was then assayed by co-incubation of these PMNs with sperm treated to induce capacitation. The BOEC supernatant significantly suppressed sperm phagocytosis by PMNs, and the LH-stimulated BOEC supernatant further suppressed phagocytosis. Importantly, in the BOEC culture, LH stimulated the secretion of prostaglandin E 2 (PGE 2 ), which dose-dependently (10 K6 , 10 K7 , and 10 K8 M) suppressed sperm phagocytosis by PMNs. Furthermore, a PGEP 2 receptor antagonist significantly abrogated the inhibition of phagocytosis by the LH-stimulated BOEC supernatant. Additionally, using scanning electron microscopy, incubation of PMNs with either PGE 2 or LH-stimulated BOEC supernatant before phagocytosis was found to prevent the formation of DNA-based neutrophil extracellular traps for sperm entanglement. The results indicate that sperm are exposed to PMNs in the oviduct and PGE 2 released into the oviduct fluid after LH stimulation may play a major role in the suppression of the phagocytic activity of PMNs for sperm via interaction with EP 2 receptors. Thus, the bovine oviduct provides a PGE 2 -rich microenvironment to protect sperm from phagocytosis by PMNs, thereby supporting sperm survival in the oviduct.Free Japanese abstract A Japanese translation of this abstract is freely available at

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“…Thus, we postulate that PMN present in the endometrium at estrus, mating or infection, might form NETs, and release nucleic and cytoplasmic proteins with immunomodulatory properties. In fact, equine spermatozoa in utero led to NETs formation (Alghamdi et al 2009). Besides, our preliminary studies show that in vitro stimulated equine PMN have NETs formation capacity when in contact with some bacteria strains obtained from mare endometritis (Rebordão et al 2011) (Fig.…”

Section: Neutrophil Extracellular Trapsmentioning

confidence: 99%

Cytokines and neutrophil extracellular traps in the equine endometrium: friends or foes?

Galvão¹,

Rebordão²,

Szóstek³

et al. 2012

PHK

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SummaryCytokines may play a dual role in the reproductive tract -either involved in physiologic processes or mediating inflammation and other pathologic processes. Physiologic secretory and angiogenic function in the equine endometrium appears to be regulated by cytokines TNFa, FasL, IFNg through their receptors. These receptors are present in glandular epithelium, and stroma cells and their mRNA expression changes throughout the estrous cycle. Besides, interleukins (IL-1a and IL-1b) and their receptors mRNA expression vary according to various degrees of endometrium inflammation (endometritis) and fibrosis (endometrosis). A novel paradigm in innate immunity and neutrophils (PMN) hyperactivation is PMN ability to cast out their DNA in response to infectious stimuli. These PMN extracellular traps (NETs) bind and kill pathogens, at the infection site. The intriguing dilemma is that even though NETs may function as a first line of defense, they also release molecules that may contribute to tissue damage. Thus, we postulate that PMN present in the endometrium at estrus, mating or infection, might form NETs, and release nucleic and cytoplasmic proteins with immunomodulatory properties. Equine PMN stimulated in vitro showed NETs formation capacity when in contact with some bacteria strains obtained from mares with endometritis, such as Streptococcus zooepidemicus, Escherichia coli and Staphylococus capitis. In this regard, even though NETs and cytokines function as an effective antimicrobial first line of defense or modulate physiologic endometrial function, respectively (friends), they may also be involved in endometrial fibrosis pathogenesis and endometrial secretory function impairment, due to enhanced NETs formation and/or a decrease on NETs degradation (foes).Keywords: Neutrophil extracellular traps; cytokines, mare, endometrium, endometritis, reproductionZytokine und "neutrophile extrazelluläre Fallen" im Endometrium des Pferdes -Freund oder Feind?Zytokine können im Reproduktionstrakt in zweifacher Hinsicht eine Rolle spielen: zum einen sind sie an physiologischen Prozessen beteiligt, zum anderen vermitteln sie bei Entzündungen oder anderen Alterationen. Die equine endometriale physiologische sekretorische und angiogene Funktion scheint durch die Zytokine TNFa, FasL, IFNg durch ihre Rezeptoren reguliert zu werden. Diese sind in den glandulären Epithelien und den Stromazellen lokalisiert, ihre mRNA Expression ist innerhalb des Zyklusgeschehens veränderlich. Darüber hinaus variieren die Interleukine (IL-1a und IL-1b) und ihre Rezeptor-mRNA-Expression in Abhängigkeit vom Grad der Endometritis und Endometrose (periglanduläre Fibrose). Ein neues Denkmodell bezüglich der angeborenen Immunität und der Hyperaktivität der neutrophilen Granulozyten (PMN) ist die Ausschüttung ihrer DNA als Reaktion auf einen infektiösen Stimulus. Diese neutrophil extracelluar traps -"Neutrophile extrazelluläre Fallen", NETs, binden und töten pathogene Keime, im Falle einer Infektion. Das faszinierende Dilemma dabei ist, dass obwohl NETs ih...

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Species-specific interaction of seminal plasma on sperm–neutrophil binding

Cited by 82 publications

References 45 publications

Leukocytes coincubated with human sperm trigger classic neutrophil extracellular traps formation, reducing sperm motility

Leukocytes coincubated with human sperm trigger classic neutrophil extracellular traps formation, reducing sperm motility

Bovine oviduct epithelial cells downregulate phagocytosis of sperm by neutrophils: prostaglandin E2 as a major physiological regulator

Cytokines and neutrophil extracellular traps in the equine endometrium: friends or foes?

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