Species-specific detection of Bipolaris sorokiniana from wheat and barley tissues

Matušinsky, Pavel; Frei, P.; Mikolasova, R.; Svačinová, Ivana; Tvarůžek, L.; Spitzer, T.

doi:10.1016/j.cropro.2010.07.013

Cited by 17 publications

(9 citation statements)

References 38 publications

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“…Conditions of the experimental year nevertheless caused the low occurrence of this pathogen. Similarly, another common pathogen Cochliobolus sativus [ 65 ] did not occur in our plots during the experiment. The evaluation of the presence and impact of pathogens on plants in the field is influenced, among other by factors as sowing procedures and the type of the preceding crop which, as a non-target host, can prevent the mass spread of infectious particles in the habitat by limiting the life cycle of the species-specific pathogen [ 60 ].…”

Section: Discussionmentioning

confidence: 88%

The Potential of Rhizoctonia-Like Fungi for the Biological Protection of Cereals against Fungal Pathogens

Bleša

Matušinsky

Sedmíková

et al. 2021

Plants

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The use of biological control is becoming a common practice in plant production. One overlooked group of organisms potentially suitable for biological control are Rhizoctonia-like (Rh-like) fungi. Some of them are capable of forming endophytic associations with a large group of higher plants as well as mycorrhizal symbioses. Various benefits of endophytic associations were proved, including amelioration of devastating effects of pathogens such as Fusarium culmorum. The advantage of Rh-like endophytes over strictly biotrophic mycorrhizal organisms is the possibility of their cultivation on organic substrates, which makes their use more suitable for production. We focused on abilities of five Rh-like fungi isolated from orchid mycorrhizas, endophytic fungi Serendipita indica, Microdochium bolleyi and pathogenic Ceratobasidium cereale to inhibit the growth of pathogenic F. culmorum or Pyrenophora teres in vitro. We also analysed their suppressive effect on wheat infection by F. culmorum in a growth chamber, as well as an effect on barley under field conditions. Some of the Rh-like fungi affected the growth of plant pathogens in vitro, then the interaction with plants was tested. Beneficial effect was especially noted in the pot experiments, where wheat plants were negatively influenced by F. culmorum. Inoculation with S. indica caused higher dry shoot biomass in comparison to plants treated with fungicide. Prospective for future work are the effects of these endophytes on plant signalling pathways, factors affecting the level of colonization and surviving of infectious particles.

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Section: Discussionmentioning

confidence: 88%

The Potential of Rhizoctonia-Like Fungi for the Biological Protection of Cereals against Fungal Pathogens

Bleša

Matušinsky

Sedmíková

et al. 2021

Plants

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“…The strains of B. sorokiniana were obtained from wheat root and leaves in Luyi County (38.48 • N, 115.08 • E), Henan province, China, with the single spore isolation (Matusinsky et al, 2010). The living strains were harvested in 10% aqueous glycerol in a 2 ml-Eppendorf tube, and then were stored at −80 • C. The fungus was cultured on PDA culture medium at 25 • C, with ampicillin resistant to bacterial growth.…”

Section: Fungal Isolatementioning

confidence: 99%

The Mitochondrial Genome of the Phytopathogenic Fungus Bipolaris sorokiniana and the Utility of Mitochondrial Genome to Infer Phylogeny of Dothideomycetes

Song

Geng

2020

Front. Microbiol.

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A number of species in Bipolaris are important plant pathogens. Due to a limited number of synapomorphic characters, it is difficult to perform species identification and to estimate phylogeny of Bipolaris based solely on morphology. In this study, we sequenced the complete mitochondrial genome of Bipolaris sorokiniana, and presented the detailed annotation of the genome. The B. sorokiniana mitochondrial genome is 137,775 bp long, and contains two ribosomal RNA genes, 12 core proteincoding genes, 38 tRNA genes. In addition, two ribosomal protein genes (rps3 gene and rps5 gene) and the fungal mitochondrial RNase P gene (rnpB) are identified. The large genome size is mostly determined by the presence of numerous intronic and intergenic regions. A total of 28 introns are inserted in eight core proteincoding genes. Together with the published mitochondrial genome sequences, we conducted a preliminary phylogenetic inference of Dothideomycetes under various datasets and substitution models. The monophyly of Capnodiales, Botryosphaeriales and Pleosporales are consistently supported in all analyses. The Venturiaceae forms an independent lineage, with a distant phylogenetic relationship to Pleosporales. At the family level, the Mycosphaerellaceae, Botryosphaeriaceae. Phaeosphaeriaceae, and Pleosporaceae are recognized in the majority of trees.

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“…2) for the first time. In our study, the molecular primers that originally have been developed for the qualitative detection of DNA of this pathogen in grain [10], were successfully adapted for its quantitative analysis. According to the obtained results, B. sorokiniana DNA was detected in 100% of the barley and oats grain samples, but only in 56% of wheat grain samples.…”

Section: Resultsmentioning

confidence: 99%

Adaptation of the quantitative PCR method for the detection of the main representatives of cereal grain mycobiota

Орина

Gavrilova

Gagkaeva

2018

Microbiology Independent Research Journal (MIR Journal)

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The content of fungal DNA and mycotoxins in cereal crops (31 varieties of wheat, oats, and barley) was quantitatively determined and used for the comparative characterization of grains. The quantitative PCR has been adapted for the analysis of the target DNA of Alternaria spp., Bipolaris sorokiniana (B. sorokiniana), Fusarium graminearum (F. graminearum), F. culmorum, and F. sporotrichioides fungi, which are often present in the mycobiota of small grain cereals. The content of the DNA of the aggressive pathogen B. sorokiniana was determined using quantitative PCR for the first time. The DNA of Alternaria fungi was found abundantly in all grain samples, but its content in the oat was significantly higher compared to barley and wheat (5 and 9 times higher, respectively). In barley grain, the content of B. sorokiniana DNA was on average significantly higher than in the grains of oats and wheat. All of the analyzed grain samples contained the DNA of F. graminearum while F. culmorum DNA was found in 70% of the oat samples and in all of the samples of barley and wheat. Mycotoxin deoxynivalenol (DON) produced by these fungi was detected in all the analyzed cereal grains in a range from 77 to 4,133 μg/kg. The DNA of F. sporotrichioides was detected in 70% of oats and 50% of barley samples but was not found in wheat. The T-2 toxin produced by this fungus was detected in 45% of all samples within a range from 2 to 89 μg/kg. The statistically significant positive correlation with the Pearson correlation coefficient (r) equal to 0.49 (p<0.05) was observed between the content of F. graminearum DNA and the amount of DON in the grain samples. Another significant positive correlation (r=0.72, p<0.01) was found between the DNA contents of Alternaria fungi and F. sporotrichioides in the grain samples. This leads to the suggestion that conditions for the growth of these fungi in grain substrates are similar.

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Species-specific detection of Bipolaris sorokiniana from wheat and barley tissues

Cited by 17 publications

References 38 publications

The Potential of Rhizoctonia-Like Fungi for the Biological Protection of Cereals against Fungal Pathogens

The Potential of Rhizoctonia-Like Fungi for the Biological Protection of Cereals against Fungal Pathogens

The Mitochondrial Genome of the Phytopathogenic Fungus Bipolaris sorokiniana and the Utility of Mitochondrial Genome to Infer Phylogeny of Dothideomycetes

Adaptation of the quantitative PCR method for the detection of the main representatives of cereal grain mycobiota

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