Species-specific antagonism of Ah receptor action by 2,2′,5,5′-tetrachloro- and 2,2′,3,3′,4,4′-hexachlorobiphenyl

Aarts, J.M.M.J.G.; Denison, Michael S.; Cox, Mary Ann; Schalk, Michel; Garrison, Patricia M.; Tullis, Kathryn; Haan, L.H.J. de; Brouwer, Abraham

doi:10.1016/0926-6917(95)90067-5

Cited by 168 publications

(96 citation statements)

References 33 publications

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“…The DR-Luc is a reporter gene assay that has been developed by Wageningen University (Aarts et al, 1995;Murk et al, 1996) and is distributed as DR-CALUX ® by Bio Detection System (BDS, NL). This system incorporates a reporter firefly gene into a cultured Rat H4IIE hepatoma cell line.…”

Section: Dr-luc As Bioassay For Dioxin-like Compoundsmentioning

confidence: 99%

The Distribution of the MusselMytilusSpecies Along the Norwegian Coast

Brooks

Farmen

2013

Journal of Shellfish Research

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Section: Dr-luc As Bioassay For Dioxin-like Compoundsmentioning

confidence: 99%

The Distribution of the MusselMytilusSpecies Along the Norwegian Coast

Brooks

Farmen

2013

Journal of Shellfish Research

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“…Foremost is the possibility of nonadditive interactions among PHHs leading to erroneous predictions of greater or lesser toxic potency of a mixture. Reported PHH interactions include antagonism Aarts et al, 1995) or synergism . The H4IIE cell bioassay can be used to address this potential problem by estimating the overall potency of a chemical extract.…”

Section: E the H4iie Cell Linementioning

confidence: 99%

The H4IIE Cell Bioassay as an Indicator of Dioxin-like Chemicals in Wildlife and the Environment

Whyte

Schmitt

Tillitt

2004

Critical Reviews in Toxicology

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ABSTRACT:The H4IIE cell bioassay has proven utility as a screening tool for planar halogenated hydrocarbons (PHHs) and structurally similar chemicals accumulated in organisms from the wild. This bioassay has additional applications in hazard assessment of PHH exposed populations. In this review, the toxicological principles, current protocols, performance criteria, and field applications for the assay are described. The H4IIE cell bioassay has several advantages over the analytical measurement of PHHs in environmental samples, but conclusions from studies can be strengthened when both bioassay and analytical chemistry data are presented together. Often, the bioassay results concur with biological effects in organisms and support direct measures of PHHs. For biomonitoring purposes and prioritization of PHH-contaminated environments, the H4IIE bioassay may be faster and less expensive than analytical measurements. The H4IIE cell bioassay can be used in combination with other biomarkers such as in vivo measurements of CYP1A1 induction to help pinpoint the sources and identities of dioxin-like chemicals. The number of studies that measure H4IIE-derived TCDD-EQs continues to increase, resulting in subtle improvements over time. Further experiments are required to determine if TCDD-EQs derived from mammalian cells are adequate predictors of toxicity to non-mammalian species. The H4IIE cell bioassay has been used in over 300 published studies, and its combination of speed, simplicity, and ability to integrate the effects of complex contaminant mixtures makes it a valuable addition to hazard assessment and biomonitoring studies.

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“…Dioxin-like compounds, expressed as 2,3,7,8-tetrachlorodibenzo-p-dioxine (TCDD) equivalents (TEQs), were determined by using a reporter gene assay DR-CALUX (Aarts et al 1995). This assay is based on rat hepatoma cells (H4IIE) stably transfected with a plasmid carrying the luciferase gene of fireflies (Photinus pyralis) as a reporter gene.…”

Section: Methodsmentioning

confidence: 99%

Are dioxin-like contaminants responsible for the eel (Anguilla anguilla) drama?

Palstra

Ginneken

Murk

et al. 2006

Naturwissenschaften

129

119

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Eel populations worldwide are dangerously close to collapsing. Our study is the first to show that current levels of dioxin-like contaminants are strong candidates because of their devastating effects on development and survival of eel embryos. Female and male silver eels were artificially stimulated to maturation and reproduction by treatment with carp pituitary extracts and hCG, respectively. During maturation of female European silver eels, about 60 g fat per kg eel is incorporated in the oocytes. Together with the fat, however, persistent organic pollutants such as dioxin-like polychlorinated biphenyls (PCBs) are incorporated too. The total dioxin-like toxic potency of the individual gonad batches was determined as 2,3,7,8-tetrachlorodibenzo-p-dioxine equivalents (TEQs), using an in vitro reporter gene assay. The observed differences in development and survival showed a significant negative correlation with the TEQ levels in the gonads, already at levels far below the maximal allowable level for fish consumption, i.e., 4 ng TEQ/kg fish. The clear inverse relationship between the TEQ level and the survival period of the fertilised eggs strongly suggests that the current levels of dioxin-like compounds seriously impair the reproduction of the European eel. The peak of the environmental levels of dioxin-like PCBs and the decline of eel coincide worldwide, further suggesting that, in addition to other threats, these contaminants contributed significantly to the current collapse in eel populations.

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Species-specific antagonism of Ah receptor action by 2,2′,5,5′-tetrachloro- and 2,2′,3,3′,4,4′-hexachlorobiphenyl

Cited by 168 publications

References 33 publications

The Distribution of the MusselMytilusSpecies Along the Norwegian Coast

The Distribution of the MusselMytilusSpecies Along the Norwegian Coast

The H4IIE Cell Bioassay as an Indicator of Dioxin-like Chemicals in Wildlife and the Environment

Are dioxin-like contaminants responsible for the eel (Anguilla anguilla) drama?

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