Species relationships in the subgenus Ceratotropis (genus Vigna) as revealed by RAPD analysis

Kaga, Akito; Tomooka, Norihiko; Egawa, Yoshinobu; Hosaka, Kazuyoshi; Kamijima, Osamu

doi:10.1007/bf00029261

Cited by 87 publications

(43 citation statements)

References 18 publications

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“…Analysis of the rDNA genes support claims that wild cowpea and mungbean are progenytors of the present day cultivated cowpea and mungbean respectively. Similarly, the analysis of the rDNA genes reveals the presence of homologous genes in Vigna radiata and Vigna mungo corroborating previous studies by Kaga et al (1996) and Goel et al (2001).…”

Section: Resultssupporting

confidence: 76%

“…The dendrogram (Figure 7) also reveals a close relationship between Vigna radiata and Vigna mungo which corroborates similar findings of Kaga et al (1996) and Goel et al (2001). Both V radiata and V mungo are in the subgenera Ceratotropis also known as Asian genus Vigna and their germplasms are useful for making crosses within the species of Vigna.…”

Section: Phylogenetic Analysissupporting

confidence: 72%

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Ribosomal DNA variation in landraces of bambara groundnut

Waziri¹,

Massawe²,

Wayah³

et al. 2013

Afr. J. Biotechnol.

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The ribosomal DNA (rDNA) is a basic component of all eukaryotic chromosomes which codes for rRNA. The rDNA is made of tandemly repeated units of 18S, 5.8S, 28S, ITS and IGS. The 18S, 5.8S and 28S are coding regions while the ITS and IGS are non coding. The rDNA evolves in a concerted manner such that rDNA units within an individual are almost completely identical making it a useful tool in taxonomic studies. The rDNA marker is used to discriminate between species in the same or different genus. Bambara groundnut is an under-utilized legume that is highly nutritious and adapted to low input farming systems. Despite its potential, Bambara groundnut is still cultivated in the form of landraces, and no true varieties have been developed. Precise estimate of genetic variation and genetic relationship is sine qua non for the development of improved cultivars. About 48 individual seeds (3 each) from seven landraces of Bambara groundnut, 9 wild/cultivated species of other legumes (cowpea, mungbean and groundnut) were used in this study. Analysis of the rDNA gene unit shows genetic variation in the species of the genus Vigna and Arachis. The UPGMA dendrogram constructed separates the plant species in this study into three (3) main clusters. The landraces of Bambara groundnut were placed in two different clusters indicating variation of rDNA gene units between the landraces. The analysis of rDNA gene in this study reveals the presence of genetic variation between the landraces of Bambara groundnut which could be used by scientists and breeders in the production of improved cultivars.

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Section: Resultssupporting

confidence: 76%

Section: Phylogenetic Analysissupporting

confidence: 72%

Ribosomal DNA variation in landraces of bambara groundnut

Waziri¹,

Massawe²,

Wayah³

et al. 2013

Afr. J. Biotechnol.

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“…Gepts (1993) suggested that molecular markers provide more useful information than morphological markers for studies on the domestication and evolution of plants. Random amplified polymorphic DNA markers (RAPD) were used in studies conducted by Cisneros and Quiros (1995) on triploid potatoes (Solanun chaucha), by Demeke and Adams (1994) on mustard and radish, by Sharma et al (1996) on lentils, by ReamonBüttner et al (1996) on beets and by Kaga et al (1996) on the genus Vigna. Amplified fragment length polymorphism markers (AFLP) have been used in studies of genetic relatedness performed by Hill et al (1996), Cervera et al (1996) and Sharma et al (1996).…”

Section: Introductionmentioning

confidence: 99%

Genetic relatedness between cassava (Manihot esculenta Crantz) and M. flabellifolia and M. Peruviana based on both RAPD and AFLP markers

Colombo

Second²,

Charrier³

2000

Genet. Mol. Biol.

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Genetic relatedness between cassava (Manihot esculentaCrantz AbstractThe taxonomy of the genus Manihot is still uncertain and the genetic origin of cassava (M. esculenta Crantz) continues to be controversial. We studied the degree of genetic relatedness between cassava and two naturally occurring species (M. flabellifolia and M. peruviana) which are probably involved in the evolution of cassava, using RAPD and AFLP molecular markers. Thirty-three clonal accessions of cassava of known genetic diversity and 15 accessions of the wild species M. flabellifolia and M. peruviana were analyzed using 92 polymorphic RAPD bands and 73 polymorphic AFLP bands. The genetic markers were unable to differentiate the two wild species, which confirms their botanical similarity. Half of the total number of amplified bands were monomorphic in all of the genotypes evaluated. The mean genetic similarity (Jaccard)

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“…Plant Breed. Genet., 9 (4): 238-246, 2015 for genome mapping experiments (Kaga et al, 1996). Genotyping of different species is necessary for characterization of different accessions of crop germplasm, testing varietal purity and registration of newly developed cultivars (Chowdhury et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Genetic Diversity in Vigna radiata (L.) Using Protein Profiling and Molecular Marker (RFLP)

Islam¹,

Mir²,

Kudesia³

2015

International J. of Plant Breeding and Genetics

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Seed storage protein profiling through SDS-PAGE and DNA Restriction Fragment Length Polymorphism (RFLPs) were surveyed in 5 accessions of mungbean (Vigna radiata L.) sampled from NBPGR Thrissur, Kerala, India, to evaluate genetic diversity. The protein profile showed a low level of polymorphism (26.66%). The similarity index calculated ranged from (42.30-50%). The dendrogram constructed revealed two clusters. Cluster 1st comprises two accessions IC 251432 and IC 251433. Cluster 2nd comprises 3 cultivars viz., IC 251431, IC 251430 and IC 251434. Cluster 2nd also revealed a 100% homology between IC 251431 and IC 251430 accessions. The RFLP analysis was carried out by single restriction enzyme EcoRI. The RFLP profile revealed a high level of polymorphism (90.90%). A total of 11 clear bands were scored. The only band with fragment size 630 kb was monomorphic while, the rest were polymorphic. Similarity index ranged from (42.82-11.11%). The UPGMA analysis divided the accessions into 2 clusters viz., cluster 1st comprising 4 accessions (IC 251431, IC 251432, IC 251433 and IC 251430). Cluster 2nd includes only one accession (IC 251434) occupying a distinct position in constructed dendrogram.

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Species relationships in the subgenus Ceratotropis (genus Vigna) as revealed by RAPD analysis

Cited by 87 publications

References 18 publications

Ribosomal DNA variation in landraces of bambara groundnut

Ribosomal DNA variation in landraces of bambara groundnut

Genetic relatedness between cassava (Manihot esculenta Crantz) and M. flabellifolia and M. Peruviana based on both RAPD and AFLP markers

Evaluation of Genetic Diversity in Vigna radiata (L.) Using Protein Profiling and Molecular Marker (RFLP)

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