Species of the genus
 Ammodytes
 (Ammodytidae) in the northwestern part of the Sea of Japan

Turanov, Sergei V.; Баланов, А. А.; Шелехов, В. А.

doi:10.1111/jai.13981

Cited by 3 publications

References 14 publications

(33 reference statements)

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The composition of northeast pacific fishes in a fish tank examined by eDNA metabarcoding

Turanov

Rutenko

2020

Preprint

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The taxonomy of fish in the northeast Pacific area has been recently revised using molecular genetic methods, including the development of a reference library of DNA fragments for species identification. Such libraries are the basis for the development of non-invasive, high-throughput methods for monitoring biodiversity using environmental DNA (eDNA). In order to validate this approach, we used a water eDNA metabarcoding technique based on 12S rRNA and COI mitochondrial fragments and assessed the composition of the twenty northeast Pacific fish species held in a fish tank at the Primorsky Aquarium (Vladivostok, Russia). Only the 12S fragment revealed data on fish-related operational taxonomic units (OTUs). Approximately 68% of the reads were classified into two species of the genus Oncorhynchus, whose shredded fillet is used for feeding. According to the taxonomic identification for the rest of the reads, 8 out of 20 fish species in the tank (40%) were identified unambiguously, while two species could not be identified. Ten taxa can be considered conditionally identifiable since they might be concealed behind a conflicting taxonomic identification at the genus or family level. In this case, an improvement of the reference library would provide resolution. We detected contamination, which may be related to both intra-laboratory contaminants occurring during DNA extraction and water intake supplying the fish tank.

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The composition of northeast pacific fishes in a fish tank examined by eDNA metabarcoding

Turanov

Rutenko

2020

Preprint

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Annotated List of Ichthyofauna of Inland and Coastal Waters of Sakhalin Island. 3. Families Priacanthidae–Sebastidae

Dyldin

Орлов

2021

J. Ichthyol.

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A complement to DNA barcoding reference library for identification of fish from the Northeast Pacific

Turanov

Kartavtsev

2021

Genome

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The seas of the North Pacific Ocean are characterized by a large variety of fish fauna, including endemic species. Molecular genetic methods, often based on DNA barcoding approaches, have been recently used to determine species boundaries and identify cryptic diversity within these species. This study complements the DNA barcode library of fish from the Northeast Pacific area. A library based on 154 sequences of the mitochondrial COI gene from 44 species was assembled and analyzed. It was found that 39 species (89%) can be unambiguously identified by the clear thresholds forming a barcoding gap. Deviations from the standard 2% threshold value resulted in detection of the species Enophrys lucasi in the sample, which is not typical for the eastern part of the Bering Sea. This barcoding gap also made it possible to identify naturally occurring low values of interspecific divergence of eulittoral taxa Aspidophoroides and the deep-sea genus Coryphaenoides. Synonymy of the genus Albatrossia in favor of the genus Coryphaenoides is suggested based on both the original and previously published data.

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Species of the genus Ammodytes (Ammodytidae) in the northwestern part of the Sea of Japan

Cited by 3 publications

References 14 publications

The composition of northeast pacific fishes in a fish tank examined by eDNA metabarcoding

The composition of northeast pacific fishes in a fish tank examined by eDNA metabarcoding

Annotated List of Ichthyofauna of Inland and Coastal Waters of Sakhalin Island. 3. Families Priacanthidae–Sebastidae

A complement to DNA barcoding reference library for identification of fish from the Northeast Pacific

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