In 2013-2014, the soil mite communities from six overgrazed grassland ecosystems located in the Trascău Mountains, Romania, were investigated. Forty-six species were identified, with 645 individuals. Some abiotic factors from soil were measured (soil temperature -T, soil water content -H, soil acidity -pH, carbon content -C, total nitrogen -Nt, and C/Nt ratio). Significant statistical differences were obtained between environmental factors. Using a canonical correspondence analysis of mite species abundance, environmental variables and habitats, strong relationships between investigated factors were established.