Species-level phylogeography and evolutionary history of the hyperdiverse marine gastropod genus Conus

Duda, Thomas F.; Kohn, Alan J.

doi:10.1016/j.ympev.2004.09.012

Cited by 134 publications

(143 citation statements)

References 35 publications

(47 reference statements)

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“…Over the last ∼30 million years, these species have evolved sophisticated predatory and defense strategies, with the elaboration of a highly organized envenomation machinery (2). Their venom apparatus is responsible for the biosynthesis and maturation of short peptide neurotoxins called conotoxins (occasionally referred to as conopeptides) that, once injected in the prey or predator (fish, molluscs, or worms), act as fast-acting paralytics.…”

mentioning

confidence: 99%

Optimized deep-targeted proteotranscriptomic profiling reveals unexplored Conus toxin diversity and novel cysteine frameworks

Lavergne

Harliwong

Jones

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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Cone snails are predatory marine gastropods characterized by a sophisticated venom apparatus responsible for the biosynthesis and delivery of complex mixtures of cysteine-rich toxin peptides. These conotoxins fold into small highly structured frameworks, allowing them to potently and selectively interact with heterologous ion channels and receptors. Approximately 2,000 toxins from an estimated number of >70,000 bioactive peptides have been identified in the genus Conus to date. Here, we describe a high-resolution interrogation of the transcriptomes (available at www.ddbj.nig.ac.jp) and proteomes of the diverse compartments of the Conus episcopatus venom apparatus. Using biochemical and bioinformatic tools, we found the highest number of conopeptides yet discovered in a single Conus specimen, with 3,305 novel precursor toxin sequences classified into 9 known superfamilies (A, I1, I2, M, O1, O2, S, T, Z), and identified 16 new superfamilies showing unique signal peptide signatures. We were also able to depict the largest population of venom peptides containing the pharmacologically active C-C-CC-C-C inhibitor cystine knot and CC-C-C motifs (168 and 44 toxins, respectively), as well as 208 new conotoxins displaying odd numbers of cysteine residues derived from known conotoxin motifs. Importantly, six novel cysteine-rich frameworks were revealed which may have novel pharmacology. Finally, analyses of codon usage bias and RNA-editing processes of the conotoxin transcripts demonstrate a specific conservation of the cysteine skeleton at the nucleic acid level and provide new insights about the origin of sequence hypervariablity in mature toxin regions.

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mentioning

confidence: 99%

Optimized deep-targeted proteotranscriptomic profiling reveals unexplored Conus toxin diversity and novel cysteine frameworks

Lavergne

Harliwong

Jones

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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“…Ultimately we excluded sequences from all species except those from members of the C. sponsalis complex and sequences from C. abbreviatus, a fairly close relative of members of this complex (Duda & Kohn 2005). We used MODELTEST 3.7 (Posada & Crandall 1998) to choose the best model of nucleotide substitution for each dataset.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

“…In Conus, an unusually diverse genus of venomous marine snails, difficult taxonomic problems involve multiple examples of species complexes (Duda & Kohn 2005;Duda & Rolán 2005). With an obligate feeding planktonic larval phase that enhances dispersal in most species (Kohn & Perron 1994) and morphological characteristics ranging from uniform to highly disparate, the problem of species complexes within Conus is dramatic.…”

Section: Introductionmentioning

confidence: 99%

Hidden diversity in a hyperdiverse gastropod genus: Discovery of previously unidentified members of a Conus species complex

Duda

Bolin

Meyer

et al. 2008

Molecular Phylogenetics and Evolution

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“…The mid-Atlantic and east Pacific barriers (wide stretches of water, difficult to cross by planktonic larvae) hindered dispersal of species across the entire width of each of the two oceans. Phylogenetic relationships among species of tropical marine taxa that occur in multiple biogeographic regions illuminate the importance of these phenomena in determining broad-scale historic patterns of divergence (Lessios et al 1999(Lessios et al , 2001(Lessios et al , 2003Colborn et al 2001;Muss et al 2001;Meyer 2003;Williams and Reid 2004;Duda and Kohn 2005;Meyer et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Connectivity of populations within and between major biogeographic regions of the tropical Pacific in Conus ebraeus, a widespread marine gastropod

Duda

Lessios

2009

Coral Reefs

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Information on genetic connectivity and structure of populations in the tropical Pacific is critical for making inferences about the origins and maintenance of diversity in this region. Sequences of the mitochondrial COI gene from 92 individuals of the trans-Pacific gastropod Conus ebraeus from eight localities spanning the tropical Pacific were analyzed to determine whether populations in the western, central, and eastern Pacific exhibit genetic structure, to examine the demographic histories of populations, and to infer patterns of gene flow. A total of 43 unique haplotypes were recovered, including a common haplotype that occurred in six of the eight populations examined. AMOVA and pairwise F-statistics showed that populations in the western and central Pacific were significantly differentiated from populations in the eastern Pacific, but no other evidence of structure. Bayesian isolation-migration (IM) analysis suggested that populations in the western and central Pacific separated from those in eastern Pacific during the Pleistocene. Examination of mismatch distributions and results from IM revealed that populations in the western and central Pacific expanded during the Pleistocene. Gene flow across the East Pacific Barrier appears to occur predominantly westward.

show abstract

Species-level phylogeography and evolutionary history of the hyperdiverse marine gastropod genus Conus

Cited by 134 publications

References 35 publications

Optimized deep-targeted proteotranscriptomic profiling reveals unexplored Conus toxin diversity and novel cysteine frameworks

Optimized deep-targeted proteotranscriptomic profiling reveals unexplored Conus toxin diversity and novel cysteine frameworks

Hidden diversity in a hyperdiverse gastropod genus: Discovery of previously unidentified members of a Conus species complex

Connectivity of populations within and between major biogeographic regions of the tropical Pacific in Conus ebraeus, a widespread marine gastropod

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