Species identification of the forensically important flies in Iwate prefecture, Japan based on mitochondrial cytochrome oxidase gene subunit I (COI) sequences

Saigusa, Kiyoshi; Takamiya, Masataka; Aoki, Yasuhiro

doi:10.1016/j.legalmed.2005.01.004

Cited by 76 publications

(55 citation statements)

References 4 publications

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“…There is a range of universal primer sets for mammalian species, such that a sample from any mammalian species will generate the same size product after amplification using the polymerase chain reaction (PCR) [62,63,71,79,[81][82][83]. The PCR product is then generally used in a sequencing reaction to determine the full sequence [29,30,64,72,[75][76][77][84][85][86][87][88][89][90]. This sequence is compared to those registered on a DNA database such as GenBank.…”

Section: Species Identification: Gene Sequence Comparisonmentioning

confidence: 99%

DNA typing in wildlife crime: recent developments in species identification

Tobe

Linacre

2010

Forensic Sci Med Pathol

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Species identification has become a tool in the investigation of acts of alleged wildlife crimes. This review details the steps required in DNA testing in wildlife crime investigations and highlights recent developments where not only can individual species be identified within a mixture of species but multiple species can be identified simultaneously. 'What species is this?' is a question asked frequently in wildlife crime investigations. Depending on the material being examined, DNA analysis may offer the best opportunity to answer this question. Species testing requires the comparison of the DNA type from the unknown sample to DNA types on a database. The areas of DNA tested are on the mitochondria and include predominantly the cytochrome b gene and the cytochrome oxidase I gene. Standard analysis requires the sequencing of part of one of these genes and comparing the sequence to that held on a repository of DNA sequences such as the GenBank database. Much of the DNA sequence of either of these two genes is conserved with only parts being variable. A recent development is to target areas of those sequences that are specific to a species; this can increase the sensitivity of the test with no loss of specificity. The benefit of targeting species specific sequences is that within a mixture of two of more species, the individual species within the mixture can be identified. This identification would not be possible using standard sequencing. These new developments can lead to a greater number of samples being tested in alleged wildlife crimes.

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Section: Species Identification: Gene Sequence Comparisonmentioning

confidence: 99%

DNA typing in wildlife crime: recent developments in species identification

Tobe

Linacre

2010

Forensic Sci Med Pathol

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“…Calliphoridae, Sarcophagidae, and Muscidae, in which 22 sequence entries concerning six species were downloaded from GenBank (see Section 2.1). Most species in this study have been reported carrion breeders [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15]. The addition of several species of no forensic relevance provides useful references for accurate species identification because it is sometimes very difficult to distinguish two different species in the same genus based on morphological characters [5].…”

Section: Introductionmentioning

confidence: 98%

“…the subunits I and II of the cytochrome oxidase, the subunit V of the NAD dehydrogenase) and nuclear encoded regions (e.g. 28s rRNA gene) have been discussed in detail [5,7,[10][11][12][13][14][15]. This study will initially investigate the possibility of the ITS2 region as the species-specific markers of necrophagous flies.…”

Section: Introductionmentioning

confidence: 99%

“…The addition of several species of no forensic relevance provides useful references for accurate species identification because it is sometimes very difficult to distinguish two different species in the same genus based on morphological characters [5]. Another reason is that congeneric species possibly share high sequence homology [10,13]. Herein, we utilized a phynetic approach to estimate the identification potential of the ITS2 region at the species level.…”

Section: Introductionmentioning

confidence: 99%

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Species identification of some common necrophagous flies in Guangdong province, southern China based on the rDNA internal transcribed spacer 2 (ITS2)

Zhong-kui

Wang

Liang

2008

Forensic Science International

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“…This makes mtDNA a better tool to determine differences in sequences of closely related species (Waugh 2007) and is, therefore, useful for molecular identification. Several working groups used the method in their countries (Wallman and Donnellan 2001;Harvey et al 2003Harvey et al , 2008Chen et al 2004;Saigusa et al 2005;Ames et al 2006;Desmyter and Gosselin 2009;Tourle et al 2009). Surprisingly, there are no records for analysing sequences of the COI gene of blowflies originating from Germany.…”

Section: Introductionmentioning

confidence: 99%

Molecular identification of forensically important blowfly species (Diptera: Calliphoridae) from Germany

2009

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Forensic entomology applies knowledge about the behaviour and ecology of insects associated to corpses to homicide investigations. It is possible to calculate a minimum post-mortem interval by determining the age of the oldest blowfly larvae feeding on a corpse. The growth rate of the larvae is highly dependent on temperature and also varies between the different blowfly species infesting a corpse. It is, thus, crucial to correctly identify the species collected from a crime scene. To increase the quality of species identification, molecular methods were applied to 53 individuals of six different species sampled in Bonn, Germany: Calliphora vicina, Calliphora vomitoria, Lucilia caesar, Lucilia sericata, Lucilia illustris, and Protophormia terraenovae. We extracted DNA and checked a 229 bp sequence within the mitochondrial cytochrome oxidase subunit I. The sequences of the local flies were aligned to published data of specimens from other countries. We also studied the practical value of the analysed DNA region for their differentiation. All species were matched correctly by a Basic Local Alignment Search Tool (BLAST) search apart from L. caesar and L. illustris. Although molecular methods are very useful-especially if it is necessary to identify small fragments of insect material or very young larvae-we propose to use it only in addition to the conventional methods.

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Species identification of the forensically important flies in Iwate prefecture, Japan based on mitochondrial cytochrome oxidase gene subunit I (COI) sequences

Cited by 76 publications

References 4 publications

DNA typing in wildlife crime: recent developments in species identification

DNA typing in wildlife crime: recent developments in species identification

Species identification of some common necrophagous flies in Guangdong province, southern China based on the rDNA internal transcribed spacer 2 (ITS2)

Molecular identification of forensically important blowfly species (Diptera: Calliphoridae) from Germany

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