Species identification of strains belonging to genus Citrobacter using the biochemical method and MALDI-TOF mass spectrometry

Kolínská, Renata; Španělová, Petra; Drevinek, Michal; Hrabák, Jaroslav; Žemličková, Helena

doi:10.1007/s12223-014-0340-4

Cited by 19 publications

(17 citation statements)

References 23 publications

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“…The species-level agreement was the same species-level result obtained by both MALDI-TOF MS instrument methods, and due to the limitations of MALDI-TOF MS, bacteria that were known to be difficult to identify accurately at the species level were compared at the complex level. Acinetobacter baumannii complex [18,19] (A. baumannii, A. pittii, A. nosocomialis, A. lactucae, and A. calcoaceticus), Citrobacter freundii complex [20] (C. freundii, C. koseri, C. braakii, C. youngae, C. werkmanii, C. sedlakii, C. amalonaticus, and C. farmeri), Enterobacter clocae complex [21] (E. cloacae, E. asburiae, E. hormaechei, E. kobei, E. ludwigii, and E. nimipressuralis), Klebsiella pneumoniae complex [22] (K. pneumoniae, K. quasipneumoniae, and K. variicola), and Burkholderia cepacia complex [23] (B. cepacia, B. cenocepacia, B. metallica, etc.) were compared in complex results.…”

Section: Comparison Of the Identification Resultsmentioning

confidence: 99%

Comparison of Autof ms1000 and Bruker Biotyper MALDI-TOF MS Platforms for Routine Identification of Clinical Microorganisms

Park

Jang

Yoon

et al. 2021

BioMed Research International

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Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is widely used in clinical microbiology laboratories because it is cost-effective, reliable, and fast. This study is aimed at comparing the identification performance of the recently developed Autof ms1000 (Autobio, China) with that of the Bruker Biotyper (Bruker Daltonics, Germany). From January to June 2020, 205 preserved strains and 302 clinical isolates were used for comparison. Bacteria were tested with duplicates of the direct transfer method, and formic acid extraction was performed if the results were not at the species level. Fungi were tested with formic acid extraction followed by ethanol extraction methods. 16S rRNA or ITS region sequence analysis was performed on isolates that could not be identified by any of the instruments and on isolates that showed inconsistent results. The time to result of each instrument was also compared. Among preserved strains, species-level identification results were obtained in 202 (98.5%) strains by the Autof ms1000 and 200 (97.6%) strains by the Bruker Biotyper. Correct identification at the species/complex level was obtained for 200 (97.6%) strains by the Autof ms1000 and for 199 (97.1%) strains by the Bruker Biotyper. Among clinical isolates, species-level identification results were obtained in 301 (99.7%) strains and 300 (99.3%) strains by the Autof ms1000 and Bruker Biotyper, respectively. Correct identification at the species/complex level was achieved for 299 (99.0%) strains by the Autof ms1000 and for 300 (99.3%) strains by the Bruker Biotyper. The time to analyze 96 spots was approximately 14 min for the Autof ms1000 and approximately 27 min for the Bruker Biotyper. The two instruments showed comparable performance for the routine identification of clinical microorganisms. In addition, the Autof ms1000 has a short test time, making it convenient for use in clinical microbiology laboratories.

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Section: Comparison Of the Identification Resultsmentioning

confidence: 99%

Comparison of Autof ms1000 and Bruker Biotyper MALDI-TOF MS Platforms for Routine Identification of Clinical Microorganisms

Park

Jang

Yoon

et al. 2021

BioMed Research International

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“…The conventional biochemical characterization and MALDI-TOF MS methods yield reliable results with approx. 90% and 95% accuracy for each methodology, respectively (Kolínská et al, 2015).…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Characterization of a multidrug resistant Citrobacter amalonaticus clinical isolate harboring blaNDM-1 and mcr-1.5 genes

Faccone

Albornoz

Tijet

et al. 2019

Infection, Genetics and Evolution

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A multidrug resistant isolate, identified as Citrobacter amalonaticus using MALDI-TOF MS and confirmed by genomic analysis, was recovered from a pediatric patient in a hospital from Buenos Aires, Argentina. By whole-genome sequencing a total of 16 resistance genes were detected, including bla NDM-1 and mcr-1.5. To the best of our knowledge this is the first description of these two genes together in a clinical isolate of the Citrobacter genus.

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“…The biochemical characteristics of the representative Citrobacter strains were performed using commercial microtest systems (Hangzhou Binhe Microorganism Reagent Co., Ltd, China). [22][23][24]. Their 16S rRNA, rpoB, fusA, pyrG and leuS gene sequences were obtained from GenBank database.…”

Section: Biochemical and Molecular Characterizationmentioning

confidence: 99%

Isolation and identification of Citrobacter spp. from the intestine of Procambarus clarkii.

Hong-lian¹,

Wang²,

Zhang³

et al. 2018

fisheries-research

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Citrobacter have caused a wide spectrum of infectious diseases in aquaculture animals and human. In previous study, pathogenic Citrobacter spp. was frequently isolated from hepatopancreas of diseased Procambarus clarkii. It suggested that the intestine might be the reservoirs of Citrobacter spp. Intestinal flora were isolated from P. clarkii with Salmonella shigella agar. 126 isolates were performed with enterobacterial repetitive intergenic consensus (ERIC) PCR. 95 strains of seven ERIC-PCR electrophoretic genotypes were identified as Citrobacter spp. by 16S rRNA gene sequence analysis and the urease gene detection of the representative strains. Eight representative strains were defined by biochemical characteristics and multilocus sequence analysis of genes fusA, leuS, rpoB and pyrG. Isolates 425C1, 731C1 and 519C4 were determined as C. freundii, C. werkmanii and a new species of C. freundii complex, respectively. Isolates 425C3, 519C1, 519C3, 519C41 and 519C53 were identified as C. braakii. C. freundii strain 425C1 and C. werkmanii strain 731C1 were verified pathogenic bacteria to P. clarkii and Kunming mice in varying degrees. To our knowledge, this is the first report of pathogenic C. werkmanii.

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Species identification of strains belonging to genus Citrobacter using the biochemical method and MALDI-TOF mass spectrometry

Cited by 19 publications

References 23 publications

Comparison of Autof ms1000 and Bruker Biotyper MALDI-TOF MS Platforms for Routine Identification of Clinical Microorganisms

Comparison of Autof ms1000 and Bruker Biotyper MALDI-TOF MS Platforms for Routine Identification of Clinical Microorganisms

Characterization of a multidrug resistant Citrobacter amalonaticus clinical isolate harboring blaNDM-1 and mcr-1.5 genes

Isolation and identification of Citrobacter spp. from the intestine of Procambarus clarkii.

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