Species identification of snapper: A food poisoning incident in Taiwan

Lin, Wenfeng; Lyu, Yao-Cheng; Wu, Ya-Jung; Lu, Chi-Huan; Hwang, Deng‐Fwu

doi:10.1016/j.foodcont.2011.11.028

Cited by 10 publications

(4 citation statements)

References 36 publications

(27 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The protein bands with a molecular weight of 45 kDa are considered as the actin fraction and three lower molecular weight bands (~30, ~20 and ~14 kDa) may correspond to the tropomyosin, myosin light chain and globin fractions. These results are in line with the results of the studies by Martinez [ 40 ] and Lin [ 41 ].…”

Section: Resultssupporting

confidence: 93%

Angiotensin I-Converting Enzyme (ACE) Inhibitory Activity and ACE Inhibitory Peptides of Salmon (Salmo salar) Protein Hydrolysates Obtained by Human and Porcine Gastrointestinal Enzymes

Darewicz

Borawska

Vegarud

et al. 2014

IJMS

View full text Add to dashboard Cite

The objectives of the present study were two-fold: first, to detect whether salmon protein fractions possess angiotensin I-converting enzyme (ACE) inhibitory properties and whether salmon proteins can release ACE inhibitory peptides during a sequential in vitro hydrolysis (with commercial porcine enzymes) and ex vivo digestion (with human gastrointestinal enzymes). Secondly, to evaluate the ACE inhibitory activity of generated hydrolysates. A two-step ex vivo and in vitro model digestion was performed to simulate the human digestion process. Salmon proteins were degraded more efficiently by porcine enzymes than by human gastrointestinal juices and sarcoplasmic proteins were digested/hydrolyzed more easily than myofibrillar proteins. The ex vivo digested myofibrillar and sarcoplasmic duodenal samples showed IC50 values (concentration required to decrease the ACE activity by 50%) of 1.06 and 2.16 mg/mL, respectively. The in vitro hydrolyzed myofibrillar and sarcoplasmic samples showed IC50 values of 0.91 and 1.04 mg/mL, respectively. Based on the results of in silico studies, it was possible to identify 9 peptides of the ex vivo hydrolysates and 7 peptides of the in vitro hydrolysates of salmon proteins of 11 selected peptides. In both types of salmon hydrolysates, ACE-inhibitory peptides IW, IY, TVY and VW were identified. In the in vitro salmon protein hydrolysates an ACE-inhibitory peptides VPW and VY were also detected, while ACE-inhibitory peptides ALPHA, IVY and IWHHT were identified in the hydrolysates generated with ex vivo digestion. In our studies, we documented ACE inhibitory in vitro effects of salmon protein hydrolysates obtained by human and as well as porcine gastrointestinal enzymes.

show abstract

Section: Resultssupporting

confidence: 93%

Angiotensin I-Converting Enzyme (ACE) Inhibitory Activity and ACE Inhibitory Peptides of Salmon (Salmo salar) Protein Hydrolysates Obtained by Human and Porcine Gastrointestinal Enzymes

Darewicz

Borawska

Vegarud

et al. 2014

IJMS

View full text Add to dashboard Cite

show abstract

“…This conclusion was based on a comparison of the protein extracted using 2% SDS and 8 M urea, as evidenced by the SDS-PAGE patterns. Lin et al (2012) identified the poisonous sample as L. bohar based on the low molecular weight region (<30.0 kD) of both the myofibrillar and sarcoplasmic patterns. Ordinary SDS-PAGE analysis proved effective in achieving species identification, as indicated by the results.…”

Section: Sodium Dodecyl Sulfate-polyacrylamide Gel Electrophoresis (S...mentioning

confidence: 99%

Electrophoretic Methods for Identifying the Species of Seafood and Its Derivatives

Tokur,

Korkmaz

2023

Foodb

View full text Add to dashboard Cite

The identification of the species of seafood and their products, whether they are fresh or cooked, is one of the key concerns of food regulations in many countries that have a significant intake of seafood. In point of fact, a commercial fraud happens when a species that is less value is intentionally substituted for a species that is more valuable, and a sanitary fraud takes place when a product that is potentially harmful is introduced into the market. A primary responsibility of veterinary inspection of seafood products is the detection of harmful species with the aim of removing them from the retail trade (Council Directive 91/493/ECC). Two efficient methods for seafood species identification are molecular biology methods and protein electrophoresis. Classic electrophoretic methods have been used for a long time to authenticate seafood; they are simple, accurate, and inexpensive compared to molecular biological methods, which are the wave of the future in food safety labs. The purpose of this article is to offer an overview of the electrophoretic methods commonly used to identify different species of seafood, including sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE), native or urea-isoelectric focusing electrophoresis (IEF), two-dimensional electrophoresis (2-DE), and capillary electrophoresis (CE).

show abstract

“…In Taiwan, 11 outbreaks of ciguatera, each involving 1–5 subjects, occurred between June 1991 and December 2008 [ 38 , 39 , 40 , 41 ]. The causative species identified included moray eel G. javanicus (April 2004), red grouper L. bohar (February 2006, December 2008).…”

Section: Reports Of Ciguateramentioning

confidence: 99%

“…In the reports reviewed here, ciguatera was clinically diagnosed, but the criteria used were rarely described [ 30 , 31 , 34 , 45 ]. Where the fish remnants were available for testing, the presence of CTX was confirmed using mouse bioassay [ 23 , 24 , 25 , 27 , 38 , 39 , 40 , 41 , 47 ], LC–MS [ 23 , 25 , 30 , 47 ] or other methods [ 26 , 31 , 34 ]. In other reports ( Table 2 ), fish remnants were not analyzed or there was no information about laboratory confirmation.…”

Section: Reports Of Ciguateramentioning

confidence: 99%

Ciguatera Fish Poisoning in East Asia and Southeast Asia

Chan

2015

Marine Drugs

View full text Add to dashboard Cite

In the coastal countries of East Asia and Southeast Asia, ciguatera should be common because of the extensive tropical and subtropical coral reefs along the coasts and in the neighboring seas with ciguatoxic fishes. An extensive search of journal databases, the Internet and the government websites was performed to identify all reports of ciguatera from the regions. Based on the official data and large published case series, the incidence of ciguatera was higher in the coastal cities (Hong Kong, Foshan, Zhongshan) of southern China than in Japan (Okinawa Prefecture). In Singapore, ciguatera appeared to be almost unknown. In other countries, only isolated cases or small case series were reported, but under-reporting was assumed to be common. Ciguatera may cause severe acute illness and prolonged neurological symptoms. Ciguatera represents an important public health issue for endemic regions, with significant socio-economic impact. Coordinated strategies to improve risk assessment, risk management and risk communication are required. The systematic collection of accurate data on the incidence and epidemiology of ciguatera should enable better assessment and management of its risk. Much more work needs to be done to define the size threshold for important coral reef fish species from different regions, above which the risk of ciguatera significantly increases.

show abstract

Species identification of snapper: A food poisoning incident in Taiwan

Cited by 10 publications

References 36 publications

Angiotensin I-Converting Enzyme (ACE) Inhibitory Activity and ACE Inhibitory Peptides of Salmon (Salmo salar) Protein Hydrolysates Obtained by Human and Porcine Gastrointestinal Enzymes

Angiotensin I-Converting Enzyme (ACE) Inhibitory Activity and ACE Inhibitory Peptides of Salmon (Salmo salar) Protein Hydrolysates Obtained by Human and Porcine Gastrointestinal Enzymes

Electrophoretic Methods for Identifying the Species of Seafood and Its Derivatives

Ciguatera Fish Poisoning in East Asia and Southeast Asia

Contact Info

Product

Resources

About