Species identification of polygonati rhizoma in China by both morphological and molecular marker methods

Abstract

Morphological markers as well as two types of molecular markers, inter-sample sequence repeat (ISSR) and start codon targeted (SCoT) are suitable for species identification of the polygonati rhizoma germplasms. In this paper, we adopted these methods for the identification of rhizomes collected from 47 areas in China. Based on their morphological characters, the collected germplasms were classified into two populations, one with alternate leaf arrangement and the other with verticillate leaf arrangement, and they were comprised of five species and fourteen subgroups. Of the five species identified: Polygonatum kingianum, P. cirrhifolium, P. alternicirrhosum, and P. sibiricum belonged to one cluster, and P. cyrtonema belonged to a different cluster. According to the analysis of both ISSR and SCoT markers, all germplasms with greater genetic similarity were classified into one group. Especially, P. sibiricum and P. cirrhifolium, which shared ∼80% similarity, were clustered together, whereas the germplasms identified as P. kingianum with ∼86% similarity formed a separate clade. P. kingianum showed a much greater genetic similarity with P. cyrtonema than with P. sibiricum. The multidimensional scaling analysis further verified the accuracy and reliability of the molecular marker-based results. Thus, both morphological and molecular methods should be combined for the differentiation of germplasms such as those of polygonati rhizoma.

Study on Hybrid Characteristics of Medicinally Used Cultivated Codonopsis Species Using Ribosomal Internal Transcribed Spacer (ITS) Sequencing

“…(S0) was closely related to C. pilosula var. modesta , rather than C. pilosula [ 14 ]. Accordingly, we speculated that it belonged to the species C. pilosula var.…”

“…A large number of molecular marker techniques, such as simple sequence repeat (SSR), single nucleotide polymorphism (SNP), and internal transcribed spacer (ITS), have been used in plant classification, identification, and analysis of genetic characteristics [ 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 ]. Among them, ITS sequence of nuclear ribosomal DNA (nrDNA) has been proved to be a helpful non-coding marker to infer hybridization events [ 5 , 16 , 17 , 18 , 19 , 20 , 21 , 22 ].…”

SCoT Markers Analysis of Genetic Diversity of Polygonatum, A Medicinal and Edible Plant

“…From the map (Fig. 1 (Jiao et al 2018). In summary, Polygonatum germplasm has higher genetic diversity.…”

“…SCoT markers have been successfully applied to research on genetic diversity of a variety of crops, owers, etc. (Tyagi et al 2020), including Polygonatum (Feng et al 2020;Jiao et al 2018).…”

Genetic diversity and SSR markers development of ancient Camellia sinensis in Sandu County of Guizhou Province

“…Therefore, samples of ancient tea germplasm from this area were collected for this study, hoping to have a certain understanding of the biodiversity of ancient tea germplasm, and to screen and get the molecular markers that can distinguish the ancient tea germplasm in this region, and to develop the molecular markers. The most effective detection method for plant variety identi cation is DNA molecular marker technology (Jiao et al 2018).…”