Species identification of partial fish larvae and juveniles in Fujian coastal areas based on cytochrome C oxidase subunit I barcoding

Chen, Xu; Shen, Changchun; Jian-di, Cai; Liu, Yong; Ma, Chao; Zhuang, Zhidong; Ge, Hui

doi:10.3724/sp.j.1118.2017.17068

Cited by 4 publications

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“…NJ phylogenetic trees constructed based on 12S rRNA cannot resolve the phylogenetic relationships among species. Other scholars (Wang et al, ; Xu et al, ) have encountered the same problem when using the CO I gene to construct phylogenetic trees. Some scholars (Wang et al, ) believe that the informative loci in the gene sequence are insufficient, and the genetic distance increases slowly with increases in the taxonomic distance.…”

Section: Discussionmentioning

confidence: 99%

Species identification of small fish in Xixuan Island coastal waters of Zhoushan using DNA barcoding

et al. 2019

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This study aimed to assess the fish species composition in Xixuan Island coastal waters and confirm the applicability of DNA barcoding in fish identification. Fish samples were caught with a gill net in December 2016 and in January, February, April, May and June 2017, and the fish species in these samples were analyzed and identified via both traditional morphological methods and DNA barcoding (mitochondrial 12S rRNA). The sample contained 1,357 individuals, the majority of which were Tridentiger barbatus (829 individuals). A total of 154 individuals were selected for DNA barcode analysis, and 147 sequences were obtained. A total of 35 species, which belonged to six orders and 16 families, were accurately identified. One species was identified to the genus level (Repomucenus), and only one was identified to the family level (Moridae). Most of the species belonged to Gobiidae (12 species). With the exception of those in Moridae, the identified species were commonly detected on the Zhejiang coast. The average Kimura two‐parameter (K2P) distances within species, genera, families, and order and between orders were 0.15%, 4.44%, 14.96%, 20.37%, and 26.20%, respectively. The intraspecific K2P distance was markedly greater than the interspecific distance. The A + T content (55.41%) was higher than the G + C content (44.58%). Phylogenetic tree analysis showed that individuals belonging to the same species were clustered together and could be clearly distinguished. Mitochondrial 12S rRNA can be effectively used for juvenile and adult fish identification and has good application prospects in fish species identification. The investigation and species identification of small fish collected monthly from Xixuan Island coastal waters are conducive to understanding the distribution characteristics and species composition of fish in the Zhoushan coastal area.

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Section: Discussionmentioning

confidence: 99%

Species identification of small fish in Xixuan Island coastal waters of Zhoushan using DNA barcoding

et al. 2019

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“…It is difficult to achieve automatic data collection in practical applications (Xu et al, 2020). Therefore, we proposed a method for dairy cow identification, in which the dairy cow target was detected by the YOLOv7 algorithm and extracted the targets' features using the imporved Resnet50 network (Zhuo et al, 2022;Wu et al, 2022;Xu et al, 2022). Then model obtain the final dairy cow ID after calculating the distance.…”

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confidence: 99%

Research on Dairy Cow Identification Methods in Dairy Farm

Liu¹,

Liu²,

Bao³

et al. 2023

IJAR

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Background: There is a large amount of occlusion in dairy farms and changes in daytime and nighttime lighting seriously affect the accuracy of traditional dairy cow identification. Methods: This paper proposed a method to dairy cow identification in dairy farm. Firstly, Resnet50 was used to extract the pattern features of dairy cows and this paper fused the fourth and fifth scale features with semantic information. Secondly, on the basis of the triplet loss, it added label smoothing loss instead of softmax cross-entropy loss to further avoid over-fitting and reduced the intra-class distance with the center loss, which improved the mean average precision (mAP) by 8.9% compared with the initial Re-identification(Reid) and the joint optimized distance calculation formula was improved the single distance measurement in the triplet loss, using two distance measurement methods to effectively improve the recognition accuracy. Finally, it was combined with pruning operation to reduce the redundant parameters of the model. Result: The experiment proves that the identification mAP of dairy cows reaches 81.2%, which is an improvement 10.9% compared to the baseline model mAP, providing a strong technical support for the subsequent dairy cow identification and target tracking system.

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DNA barcoding of fishes from Zhoushan coastal waters using mitochondrial COI and 12S rRNA genes

et al. 2023

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Species identification of partial fish larvae and juveniles in Fujian coastal areas based on cytochrome C oxidase subunit I barcoding

Cited by 4 publications

References 0 publications

Species identification of small fish in Xixuan Island coastal waters of Zhoushan using DNA barcoding

Species identification of small fish in Xixuan Island coastal waters of Zhoushan using DNA barcoding

Research on Dairy Cow Identification Methods in Dairy Farm

DNA barcoding of fishes from Zhoushan coastal waters using mitochondrial COI and 12S rRNA genes

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