Species identification of Mycobacterium abscessus subsp. abscessus and Mycobacterium abscessus subsp. bolletii using rpoB and hsp65, and susceptibility testing to eight antibiotics

Nie, Wenjuan; Duan, Hongfei; Huang, Hairong; Lu, Yu; Bi, Dapeng; Chu, Naihui

doi:10.1016/j.ijid.2014.02.014

Cited by 58 publications

(56 citation statements)

References 33 publications

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“…Both the hsp65 and rpoB genes have been widely used in the identification of M . abscessus complex strains [43, 44]. Therefore, in this study, we used phylogenetic analysis based on sequences of a total of nine genes, including hsp65 , rpoB and the seven MLST genes for the genotypic characterization of the 6 Rec-mas strains.…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic analysis of Mycobacterium massiliense strains having recombinant rpoB gene laterally transferred from Mycobacterium abscessus

Kim

et al. 2017

PLoS ONE

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Recent multi locus sequence typing (MLST) and genome based studies indicate that lateral gene transfer (LGT) events in the rpoB gene are prevalent between Mycobacterium abscessus complex strains. To check the prevalence of the M. massiliense strains subject to rpoB LGT (Rec-mas), we applied rpoB typing (711 bp) to 106 Korean strains of M. massiliense infection that had already been identified by hsp65 sequence analysis (603 bp). The analysis indicated 6 smooth strains in M. massiliense Type I (10.0%, 6/60) genotypes but no strains in M. massiliense Type II genotypes (0%, 0/46), showing a discrepancy between the 2 typing methods. Further MLST analysis based on the partial sequencing of seven housekeeping genes, argH, cya, glpK, gnd, murC, pta and purH, as well as erm(41) PCR proved that these 6 Rec-mas strains consisted of two distinct genotypes belonging to M. massiliense and not M. abscessus. The complete rpoB sequencing analysis showed that these 6 Rec-mas strains have an identical hybrid rpoB gene, of which a 478 bp partial rpoB fragment may be laterally transferred from M. abscessus. Notably, five of the 6 Rec-mas strains showed complete identical sequences in a total of nine genes, including the seven MLST genes, hsp65, and rpoB, suggesting their clonal propagation in South Korea. In conclusion, we identified 6 M. massiliense smooth strains of 2 phylogenetically distinct genotypes with a specific hybrid rpoB gene laterally transferred from M. abscessus from Korean patients. Their clinical relevance and bacteriological traits remain to be elucidated.

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Section: Discussionmentioning

confidence: 99%

Phylogenetic analysis of Mycobacterium massiliense strains having recombinant rpoB gene laterally transferred from Mycobacterium abscessus

Kim

et al. 2017

PLoS ONE

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“…Then colonies were used for species identification based on 16S rRNA sequence analysis. Preparation of the PCR reaction mixture and amplification were done as described previously [5, 6]. PCR products were purified and sequenced by the BGI corporation, using forward and reverse primers.…”

Section: Methodsmentioning

confidence: 99%

“…DST of these NTM isolates was done as described previously [6]. To M. abscessus , plates were submitted to an extended incubation of 14 days' incubation at 30°C.…”

Section: Methodsmentioning

confidence: 99%

Species Identification and Clarithromycin Susceptibility Testing of 278 Clinical Nontuberculosis Mycobacteria Isolates

Nie

Duan

Huang

et al. 2015

BioMed Research International

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Purpose of this paper is to analyze different species' proportion of nontuberculosis mycobacteria (NTM) and susceptibility to clarithromycin of different species. 278 clinical NTM isolates were identified into species by using 16S rRNA, rpoB and hsp65. Then clarithromycin susceptibility testing against different species was done separately, using microplate Alamar Blue assay. Finally, resistance isolates' erm(41) of M. abscessus were sequenced in order to analyze mechanisms for clarithromycin resistant. In this test, 131 isolates (47%) belonged to M. avium complex (MAC), and 70 isolates (25%) belonged to M. abscessus. Nearly all the M. abscessus subsp. abscessus resistant to clarithromycin had T28 in erm(41). However, all the M. abscessus subsp. abscessus susceptible to clarithromycin had C28 in erm(41). In this study, we find that MAC was the most common pathogens of NTM, and the second one was M. abscessus. However, M. chelonei, M. fuerth, and M. gordon were rare. Clarithromycin had a good inhibition activity against all the NTM species except M. abscessus subsp. abscessus. The erm(41) genotype is of high relevance to clarithromycin resistance.

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“…It is well known that M. abscessus subspecies exhibit different clinical and epidemiological features (18,19). M. massiliense is more susceptible to antibiotics but is also more often associated with clinical infections.…”

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confidence: 99%

Support from Phylogenomic Networks and Subspecies Signatures for Separation of Mycobacterium massiliense from Mycobacterium bolletii

2015

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bMycobacterium abscessus subspecies classification has important clinical implications. We used phylogenomic network and amino acid analyses to provide evidence for the separation of Mycobacterium bolletii and Mycobacterium massiliense into two distinct subspecies which can potentially be differentiated rapidly by their protein signatures. Mycobacterium abscessus has become one of the most frequently isolated nontuberculous mycobacterium (NTM) in clinical laboratories. It is associated with chronic, recurrent infections that are difficult to treat, partly because of its resistance to many of the usual medications for NTM infections. This species was previously divided into three subspecies (M. abscessus, M. massiliense, and M. bolletii) based on biological and genetic differences (1-3). Currently, however, only two subspecies are recognized; while M. abscessus is retained as Mycobacterium abscessus subsp. abscessus, M. massiliense and M. bolletii are placed in the same subspecies designated Mycobacterium abscessus subsp. bolletii (4). This tenuous merging of M. massiliense and M. bolletii is still being debated as recent publications support the previous three-subspecies classification (5). Here, we present more evidence for the retention of the former three-subspecies taxonomic division, which correlates better with the expected treatment outcomes in infected patients (6).(This research was conducted by J. L. Tan in partial fulfillment of the requirements for a Ph.D. from University of Malaya, Kuala Lumpur, Malaysia.) For our genomic and amino acid analyses, we used 12 genomes from strains isolated in the Diagnostic Microbiology Laboratory

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Species identification of Mycobacterium abscessus subsp. abscessus and Mycobacterium abscessus subsp. bolletii using rpoB and hsp65, and susceptibility testing to eight antibiotics

Cited by 58 publications

References 33 publications

Phylogenetic analysis of Mycobacterium massiliense strains having recombinant rpoB gene laterally transferred from Mycobacterium abscessus

Phylogenetic analysis of Mycobacterium massiliense strains having recombinant rpoB gene laterally transferred from Mycobacterium abscessus

Species Identification and Clarithromycin Susceptibility Testing of 278 Clinical Nontuberculosis Mycobacteria Isolates

Support from Phylogenomic Networks and Subspecies Signatures for Separation of Mycobacterium massiliense from Mycobacterium bolletii

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