Species Identification of Larval Fish in Hawaiian Waters Using DNA Barcoding

Xing, Bingpeng; Wang, Chunguang; Wu, Qiong; Wang, Yanguo; Chen, Xiaoyin; Sun, Rong; Zhang, Zhilan; Xiang, Peng

doi:10.3389/fmars.2022.825395

Cited by 5 publications

(5 citation statements)

References 39 publications

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“…In Hawaiian waters 92 fish larvae were identified using the COI-5P marker with a mean intraspecific divergence of 0.72% vs . a mean interspecific but congeneric divergence of 25.9%, but even with these genetic divergences, 52 specimens could only be assigned to the genus level because of the low values of identity observed when comparing query sequences with the reference database ( Xing et al, 2022 ). The difference between intra and interspecific genetic distances observed here for Labrisomus species, support the phylogenetic position and taxonomic identification of larvae sequences of this genus.…”

Section: Discussionmentioning

confidence: 99%

“…Despite good validation of molecular techniques for species identification of ichthyoplankton in the last decade, all these studies stress that the reliability of this approach depends on representative databases with correct taxonomic identifications of the reference data ( Ko et al, 2013 ; Azmir et al, 2017 ; Mateos-Rivera et al, 2020 ; Xing et al, 2022 ). Using two databases to identify species in the similarity analyses in the present study (NCBI and BOLD) helped to achieve reliable identification for almost all specimens (68 specimens presenting >98% of similarity).…”

Section: Discussionmentioning

confidence: 99%

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Identification of pre-flexion fish larvae from the western South Atlantic using DNA barcoding and morphological characters

Pozzobon,

Ready,

Di Dario

et al. 2024

PeerJ

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Knowledge on species composition is the first step necessary for the proper conservation and management of biological resources and ecologically relevant species. High species diversity and a lack of diagnostic characters for some groups can impose difficulties for taxonomic identification through traditional methodologies, and ichthyoplankton (fish larvae and eggs) are a good example of such a scenario. With more than 35.000 valid species of fishes worldwide and overall similar anatomies in early developmental stages in closely related groups, fish larvae are often hard to be identified at the species or even more encompassing taxonomic levels. To overcome this situation, molecular techniques have been applied, with different markers tested over the years. Cytochrome c oxidase I (COI) is the most commonly used marker and now has the broadest public reference libraries, providing consistent results for species identification in different metazoan studies. Here we sequenced the mitochondrial COI-5P fragment of 89 fish larvae collected in the Campos Basin, coastal southeastern Brazil, and compared these sequences with references deposited in public databases to obtain taxonomic identifications. Most specimens identified are species of the Blenniiformes, with Parablennius and Labrisomus the most frequently identified genera. Parablennius included two species (P. marmoreus and P. pilicornis), while Labrisomus included three species (L. cricota, L. conditus and L. nuchipinnis). Anatomy of these molecularly identified specimens were then analyzed with the intention of finding anatomical characters that might be diagnostically informative amongst the early development stage (pre-flexion) larvae. Ventral pigmentation patterns are proposed as useful markers to identify Labrisomus species. However, additional specimens are needed to confirm if the character holds stability through the geographic distribution of the species.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Identification of pre-flexion fish larvae from the western South Atlantic using DNA barcoding and morphological characters

Pozzobon,

Ready,

Di Dario

et al. 2024

PeerJ

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“…Figure 4 presents a comparison of the fish larval abundance at the family level in the selected sites for a two-year sampling period. Among the study sites, the Moheshkhalipara coast exhibited the highest number of families (21) followed by the Rezu Khal Estuary (19), Bakkhali River Estuary (15), and Naf River Estuary (12). The Naf River Estuary displayed the least number of larval families among the stations.…”

Section: Spatial and Temporal Variation In Fish Abundance And Diversitymentioning

confidence: 91%

“…For instance, meristic traits like myomere, vertebrae, and fin ray counts are particularly useful as they may be species-specific [17,18]. The accurate identification and classification of fish larval species are critical for effective fisheries management as they allow us to estimate parent population size, predict future recruitment, and identify critical spawning and nursery sites [19].…”

Section: Introductionmentioning

confidence: 99%

Larval Fish Assemblages in Coastal Waters of Bangladesh: Spatial and Seasonal Dynamics

Nahid,

Rana,

Sultana

et al. 2024

Conservation

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The distribution of fish larvae in estuaries is crucial for comprehending the functioning of these ecosystems and their role as nursery grounds for marine fish species. Data regarding larval fish assemblages in the coastal waters of Bangladesh are currently scarce. This study examines the spatial and seasonal dynamics of larval fish communities in these waters using a two-year sampling effort with a bongo net. This study investigates the diversity, abundance, and distribution of larval fishes at four sites (Bakkhali River Estuary, Moheshkhalipara, Naf River Estuary, and Rezu Khal Estuary) on the southeastern coast of Bangladesh. A total of 10,387 fish larvae representing 25 families were identified. Clupeidae (42.9%), Engraulidae (35.5%), Ambassidae (10.1%), Myctophidae (2.1%), and Gobiidae (1.9%) were the five most dominant families based on the larval catch composition. Marked seasonal differences in the larval fish abundance were observed. However, these differences were not observed when comparing the four sampled sites. Additionally, the correlation between environmental variables and the larval abundance indicated that salinity negatively influences (p < 0.001) the larval fish abundance. This is the first comprehensive investigation of larval fish assemblages in the coastal waters of Bangladesh. The findings of this study enhance our understanding of the nursery habitat requirements for the early development stages of both migratory and resident species in subtropical coastal waters, providing valuable information for potential applications in management and conservation efforts.

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“…Larval fish can provide the fundamental source for the replenishment and sustainable utilization of fish stocks (Panprommin et al, 2020;Panprommin et al, 2021;Huang et al, 2022). Accurate identification and classification of larval fish species are crucial for effective fisheries management, as it enables the estimation of parent stocks and future recruitment, as well as the prediction of important spawning and nursing grounds (Baumgartner et al, 2004;Xing et al, 2022). Traditional morphological identification methods based on morphological features such as spination, body shape, fin placement, and photophores often yield misidentification due to distinct characteristics between larval and adult fish and developmental variations (Azmir et al, 2017;Sheraliev and Peng, 2021).…”

Section: Introductionmentioning

confidence: 99%

Assessing larval fish diversity and conservation needs in the Luzon strait using DNA barcoding

Li,

Chen,

et al. 2023

Front. Mar. Sci.

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IntroductionAccurate species identification and biodiversity assessment of larval fish are essential for effective management and conservation of fisheries resources, as it allows for the estimation of parent stocks, assessment of future recruitment, and prediction of spawning and nursing grounds. However, traditional morphology-based identification methods have inherent limitations, highlighting the need for accurate and efficient techniques to address these challenges effectively. The Luzon Strait, a crucial channel connecting the South China Sea to Western Pacific Ocean, is renowned for its fish biodiversity. However, our knowledge of the biodiversity status of larval fish species in this region remains insufficient.MethodsHere we employed DNA barcoding to assess larval fish species diversity in the Luzon Strait and adjacent waters. We investigated the species composition, diversity, and geographical distribution of larval fish communities in the region. Moreover, we assessed habitat types, human uses, and IUCN conservation status of each larval fish species.ResultsA total of 385 larval fish individuals were collected from 15 stations, and 354 individuals were successfully barcoded and identified, representing 147 species from 93 genera, 44 families, and 22 orders. The interspecific Kimura 2-parameter (K2P) divergence exhibited a significant increase of approximately 55-fold higher than intraspecific divergence. The phylogenetic neighbor-joining tree confirmed the distinct lineages for each taxonomic level, demonstrating the feasibility of DNA barcoding. We observed notable variations in fish species diversity and community composition among sampling stations. Non-metric multidimensional scaling analysis revealed greater diversity and dissimilarity of larval fish community compositions in the western regions compared to the eastern regions. This pattern corresponded to the grouping based on the path of the Kuroshio current, suggesting its influence on the fish community structure. Additionally, economically valuable species were identified at these stations, highlighting their ecological significance as potential spawning or nursery grounds for larval fish. We also examined the habitat type, human use, and conservation status of each larval fish species, providing comprehensive insights into their ecological significance and conservation needs.DiscussionThe establishment of a local DNA sequences database through DNA barcoding will greatly enhance the accuracy of species identification in environmental DNA (eDNA) metabarcoding applications. Altogether, this study offers valuable information for identifying important spawning and nursing grounds of fish populations, thereby supporting sustainable management and conservation of fisheries resources in this region.

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Species Identification of Larval Fish in Hawaiian Waters Using DNA Barcoding

Cited by 5 publications

References 39 publications

Identification of pre-flexion fish larvae from the western South Atlantic using DNA barcoding and morphological characters

Identification of pre-flexion fish larvae from the western South Atlantic using DNA barcoding and morphological characters

Larval Fish Assemblages in Coastal Waters of Bangladesh: Spatial and Seasonal Dynamics

Assessing larval fish diversity and conservation needs in the Luzon strait using DNA barcoding

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