Species identification of Eubacterium, Clostridium, and obligately anaerobic Gram-positive cocci and Gram-negative rods isolated from human carious dentine.

With the adoption of an anaerobic glove box system, we isolated and identified the predominant micro-organisms in lesions of carious dentin from four permanent third molars. The overwhelming majority of the micro-organisms isolated from both shallow and deep layers of carious dentin were obligate anaerobes, suggesting that conditions in the dentin were strictly anaerobic. The predominant obligate anaerobes were Gram-positive rods which were identified as members of the following genera: Propionibacterium, Eubacterium, Arachnia, Lactobacillus, Bifidobacterium, and Actinomyces. Clostridia, Gram-negative rods (Bacteroides and Fusobacterium) and Gram-positive cocci (Peptococcus, Peptostreptococcus, and Streptococcus) were also isolated, although they constituted a minor part of the flora.

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Predominant Obligate Anaerobes in Human Carious Dentin

Hoshino

1985

J Dent Res

190

128

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Characterization of bacterial deposits formed in vivo on hydrogen-ion-sensitive field-effect transistor electrodes and enamel surfaces.

Hoshino¹,

Sato²,

Sasano³

et al. 1989

Jpn J Oral Biol

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Abstract:In order to clarify the bacteriological similarities of bacterial deposits formed on transistor pH electrodes (pH-ISFET) and enamel surfaces in vivo, bacteria were allowed to accumulate on indwelling electrodes in four human mouths, and the the predominant bacteria were then isolated and characterized. Both the total number of bacteria accumulated per unit area and the population of predominant bacteria were similar for the electrodes and enamel surfaces, indicating that pH changes in the bacterial deposits formed on the electrodes can be representative of those occurring in natural dental plaque formed on enamel surfaces. Obligate anaerobes were predominant (68%) among the 346 isolates, and almost all the isolates were acidogenic. This may be a good reason why rapid pH-drop to pH 4 level was observed in every subject when 1% glucose or sucrose was applied to the bacterial deposits.

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Species identification of Eubacterium, Clostridium, and obligately anaerobic Gram-positive cocci and Gram-negative rods isolated from human carious dentine.

Cited by 2 publications

References 8 publications

Predominant Obligate Anaerobes in Human Carious Dentin

Predominant Obligate Anaerobes in Human Carious Dentin

Characterization of bacterial deposits formed in vivo on hydrogen-ion-sensitive field-effect transistor electrodes and enamel surfaces.

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