Species identification of dermatophytes isolated from human superficial fungal infections by conventional and molecular methods

Taha, Mohamed; Elfangary, Mona M.; Essa, Sabry; Younes, A.

doi:10.1097/01.ewx.0000499598.84966.cb

Cited by 6 publications

(8 citation statements)

References 22 publications

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“…One hundred (100) samples of infected skin, nail, and scalp craping were collected from selected study participants attending Community HIV-AIDS Initiative (CHAI) and Dermatology Clinic through the help of a volunteer who is registered health personnel (medical laboratory personnel). Samples were collected according to the method described by Taha et al [17] with little modification. The sites of infections were first cleaned with surgical spirit, and scales from the skin lesions were collected by scraping outwards with a blunt scalpel from the edge of the lesion.…”

Section: Methodsmentioning

confidence: 99%

Prevalence of Dermatophytosis and Antifungal Activity of Ethanolic Crude Leaf Extract ofTetradenia ripariaagainst Dermatophytes Isolated from Patients Attending Kampala International University Teaching Hospital, Uganda

Kakande

Batunge

Eilu

et al. 2019

Dermatology Research and Practice

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Dermatophyte infections are a global health problem but neglected in Uganda. This work aimed at determining prevalence of dermatophytosis and antifungal activity of ethanolic crude leaf extract of Tetradenia riparia against dermatophytes isolated from patients attending Kampala International University Teaching Hospital (KIU-TH), Uganda. A total of 100 samples of skin and nail scrapings were collected and processed using standard microscopy (KOH) and cultural methods. T. riparia leaves were collected and processed with 95% ethanol using standard extraction method. The crude leaves ethanolic extract was tested against three dermatophytes: Trichophyton tonsurans, T. mentagrophyte, and Microsporum audouinii using modified agar well diffusion method. Minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of the ethanolic leaves crude extract were also determined using broth tube dilution and culture, respectively. Out of 100 samples collected, 49 (49%, 95%CI: 0.3930-0.5876) were found positive for microscopy. The prevalence of dermatophytosis was significantly (p=0.001) associated with age groups of participants with higher infection among those aged 11-20 and 21-30 years with 75.0% each. Out of the 49 that were positive by microscopy, 28 (57.15%, 95% CI: 0.1987-0.3739) were positive by culture. Thirty-one (31) fungal isolates were obtained which included both dermatophyte and non-dermatophyte fungi. T. verrucosum had highest distribution 6 (19.35%) among dermatophytes species while Aspergillus spp. were found to have highest distribution 7 (22.58%) among non-dermatophyte species. The result of the antidermatophytic test showed that T. riparia ethanolic crude leaves extract had activity against tested dermatophytes at 1 g/ml. MIC and MFC of the crude extract of T. riparia against tested dermatophytes ranged from 62.5 to 250 mg/ml and 125 to 500 mg/ml, respectively. The findings of this study reported the presence of dermatophytes causing dermatophytosis among patients attending KIU-TH. The results of the current study showed that T. riparia leaves ethanolic crude extract has antidermatophytic activity against tested dermatophytes.

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Section: Methodsmentioning

confidence: 99%

Prevalence of Dermatophytosis and Antifungal Activity of Ethanolic Crude Leaf Extract ofTetradenia ripariaagainst Dermatophytes Isolated from Patients Attending Kampala International University Teaching Hospital, Uganda

Kakande

Batunge

Eilu

et al. 2019

Dermatology Research and Practice

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“…Conventionally, the identification of dermatophytes including Microsporum spp., especially M. canis , usually depends on phenotypical features, such as direct microscopy and in‐vitro culture. Morphological and physiological characteristics can frequently vary; in fact, the phenotypic features can be easily influenced by external factors such as temperature and the medium used (Taha et al., 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Molecular diagnosis of dermatophyte isolates from canine and feline dermatophytosis in Northeast Iran

Lavari

Eidi

Soltani

2021

Veterinary Medicine & Sci

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Background Dermatophytes are the most common causes of cutaneous fungal diseases. Dermatophytosis is a common skin disorder in dogs and cats. Species identification of these fungi is important from a therapeutic and epidemiological aspect. Conventional methods used to identify dermatophyte species are often lengthy and may be inefficient in many circumstances. Recently broad varieties of several molecular DNA‐based techniques were successfully utilised for species detection of dermatophytes. Objectives The aim of this study was to determine the molecular detection of dermatophyte isolates from canine and feline dermatophytosis in Mashhad, Iran. Methods Thirty dermatophytes isolated from dogs and cats with skin lesions and one standard strain of Microsporum canis were cultured onto Mycosel agar, and then internal transcribed spacer (ITS) region of the ribosomal DNA was amplified using the universal fungal primers ITS1 and ITS4. PCR products were subjected to sequencing and sequence analysis. Results Based on the sequencing of the ITS1‐5.8S‐ITS2 region on all samples, all the studied strains were M. canis and their sexual stage (teleomorph) was Arthroderma otae. Conclusions Microsporum canis was the only species found among dogs and cats, and its high prevalence can increase the rate of transmission to humans. In practice, ITS‐PCR, with sequence analysis, is a useful and reliable method to identify and differentiate various pathogenic species, and it can be used in clinical and epidemiological fields, even for the rapid diagnosis of dermatophyte species that are closely interrelated.

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“…The reference of macroscopic and microscopic identification of the fungi was revealed in Table 1. The macromorphological examination is based on the reported consistency, surface color, reverse color, and change in the DTM color (Moriello, 2001;Taha et al, 2017). Micromorphological examinations: Using colony in DTM, and then examined by a clear view window under the microscope for hyphae and conidia; in other media, the first subculture was done on dermal-agar, and then growth samples were placed in a slide with drops of lactophenol cotton blue (Hi-Media, Kennett Square, Pennsylvania, USA), covered with a cover and examined under the microscope for identification of hyphae, macroconidia, and microconidia (Paryuni et al, 2022).…”

Section: Methodsmentioning

confidence: 99%

Dermatophytosis in Cats: Clinical Signs and Identification of Etiological Agent

Paryuni¹,

Indarjulianto²,

Untari³

et al. 2023

AAVS

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Fungal infection by dermatophytosis is a zoonotic disease distributed globally and well concern in public health. This study aimed to investigate clinical signs and the etiological agents of dermatophytosis in cats in Yogyakarta. A total of 69 cats were observed in this study. Physical examination, by using Wood's lamp, and microscopic examination of hyphae on the skin was used for preliminary screening of dermatophytosis. Identification of fungi was conducted by scrapping area infection of the skin and then cultured on Dermatophyte Test Medium (DTM) for macroscopic and microscopic examination. The results of this study demonstrated that dermatophytosis in cats is characterized by the presence of multifocal alopecia, scale, pruritus, and erythema on the skin with a specific formation. The dominant clinical lesion was alopecia followed by crust and erythema, stated at 75.8%, 42.8%, and 1.86% respectively. Physical examination showed that 55 cats (79.7%) were positive fungal infections by using direct Wood's lamp examination and skin scrappings. Moreover, the identification of agents demonstrated that 58 (84%) and 11 (16%) of cats were infected by M. canis and T. mentagrophytes, respectively. Besides clinical signs, Wood's lamp, and microscopic examination, the gold standard for M. canis idenification is Dermatophyte Test Medium.

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Species identification of dermatophytes isolated from human superficial fungal infections by conventional and molecular methods

Cited by 6 publications

References 22 publications

Prevalence of Dermatophytosis and Antifungal Activity of Ethanolic Crude Leaf Extract ofTetradenia ripariaagainst Dermatophytes Isolated from Patients Attending Kampala International University Teaching Hospital, Uganda

Prevalence of Dermatophytosis and Antifungal Activity of Ethanolic Crude Leaf Extract ofTetradenia ripariaagainst Dermatophytes Isolated from Patients Attending Kampala International University Teaching Hospital, Uganda

Molecular diagnosis of dermatophyte isolates from canine and feline dermatophytosis in Northeast Iran

Dermatophytosis in Cats: Clinical Signs and Identification of Etiological Agent

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