Species identification and strain typing of Malassezia species stock strains and clinical isolates based on the DNA sequences of nuclear ribosomal internal transcribed spacer 1 regions

Makimura, Koichi; Tamura, Yoshiko; Kudo, Michinari; Uchida, Katsuhisa; Saito, Hiroyoshi; Yamaguchi, Hideyo

doi:10.1099/0022-1317-49-1-29

Cited by 115 publications

(71 citation statements)

References 23 publications

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“…Malassezia sympodialis, M. globosa and M. restricta have been reported to be more dominant on the skin of AEDS patients or healthy individuals (11,24,(27)(28)(29)(30). The IgE antibodies are also more frequently found with these species in AEDS patients (26).…”

Section: Discussionmentioning

confidence: 89%

IgE‐reactivity to seven Malassezia species

Zargari

Midgley

Bäck

et al. 2003

Allergy

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Section: Discussionmentioning

confidence: 89%

IgE‐reactivity to seven Malassezia species

Zargari

Midgley

Bäck

et al. 2003

Allergy

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“…To overcome the limits of morphological assessment, recent studies have used a variety of molecular methods such as the nested polymerase chain reaction (PCR) 12 , real-time PCR 13 , pulsed field gel electrophoresis (PFGE) 14 , amplified fragment length polymorphism (AFLP) 15,16 , denaturing gradient gel electrophoresis (DGGE) 16 , random amplification of polymorphic DNA (RAPD) 16,17 , single strand conformation polymorphism (SSCP) 18 , terminal fragment length polymorphism (tFLP) 19 , restriction fragment length polymorphism (RFLP) [20][21][22][23] , and sequencing analysis 24 .…”

Section: Introductionmentioning

confidence: 99%

Comparison of Nested PCR and RFLP for Identification and Classification of Malassezia Yeasts from Healthy Human Skin

Song

Lee

et al. 2009

Ann Dermatol

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Background: Malassezia yeasts are normal flora of the skin found in 75∼98% of healthy subjects. The accurate identification of the Malassezia species is important for determining the pathogenesis of the Malassezia yeasts with regard to various skin diseases such as Malassezia folliculitis, seborrheic dermatitis, and atopic dermatitis. Objective: This research was conducted to determine a more accurate and rapid molecular test for the identification and classification of Malassezia yeasts. Methods: We compared the accuracy and efficacy of restriction fragment length polymorphism (RFLP) and the nested polymerase chain reaction (PCR) for the identification of Malassezia yeasts. Results: Although both methods demonstrated rapid and reliable results with regard to identification, the nested PCR method was faster. However, 7 different Malassezia species (1.2%) were identified by the nested PCR compared to the RFLP method. Conclusion: Our results show that RFLP method was relatively more accurate and reliable for the detection of various Malassezia species compared to the nested PCR. But, in the aspect of simplicity and time saving, the latter method has its own advantages. In addition, the 26S rDNA, which was targeted in this study, contains highly conserved base sequences and enough sequence variation for inter-species identification of Malassezia yeasts. (Ann Dermatol 21(4) 352

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“…In contrast, other studies, mainly carried out in areas with tropical or subtropical climates, showed a predominance of M. furfur in PV lesions. 2 Makimura et al 27 in Japan, by molecular identification of the species, observed M. furfur and M. sympodialis as the predominant agents of PV, but not M. globosa. Species different from M. globosa, which is mainly observed in Northern countries, might predominate in PV in other climates.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Malassezia species in patients with pityriasis versicolor in Rosario, Argentina

Ramadán

Sortino

Bulacio

et al. 2012

Revista Iberoamericana de Micología

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a b s t r a c tBackground: Malassezia species are considered opportunistic yeasts of increasing clinical importance. These lipophilic yeasts are associated with various human diseases, especially pityriasis versicolor (PV), a chronic superficial scaling dermatomycosis. Aims: The aim of this study was to isolate, identify and analyze the distribution of the different species of Malassezia in patients with PV in Rosario city (Argentina). Methods: A total of 264 clinical samples were studied. Isolates were identified on the basis of microscopic observation of cells, and physiological properties, such as the presence of catalase, ability to use Tween compounds, splitting of esculin, and morphology, color and precipitate production on chromogenic agar CHROMagar-Malassezia medium (CHROMM). Results: The highest prevalence of PV in this study was observed in the 25-to 45-year-old group. No differences were found in the development of PV between sexes. The most affected areas of body were the trunk and face. Malassezia sympodialis (51%) was the most commonly isolated species, followed in frequency by M. globosa (40%), Malassezia furfur (7%), Malassezia obtusa (1%) and Malassezia slooffiae (1%). Conclusions: The success for a correct identification of these yeasts is important to improve our knowledge about their epidemiological role in PV and also to detect the appearance of strains which are resistant to the commonly used antifungal drugs.

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Species identification and strain typing of Malassezia species stock strains and clinical isolates based on the DNA sequences of nuclear ribosomal internal transcribed spacer 1 regions

Cited by 115 publications

References 23 publications

IgE‐reactivity to seven Malassezia species

IgE‐reactivity to seven Malassezia species

Comparison of Nested PCR and RFLP for Identification and Classification of Malassezia Yeasts from Healthy Human Skin

Prevalence of Malassezia species in patients with pityriasis versicolor in Rosario, Argentina

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