Species diversity of magnetotactic bacteria from the Ol’khovka River, Russia

Dziuba, Marina; Kolganova, T. V.; Горленко, В. М.; Кузнецов, Б. Б.

doi:10.1134/s0026261713030028

Cited by 13 publications

(10 citation statements)

References 19 publications

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“…High-throughput 16S rRNA gene sequencing (Illumina) of three replicate samples from the pond sediment identified uncultivated taxa corresponding to Magnetococcus and the rod-shaped organisms "Candidatus Magnetobacterium bavaricum" and "Candidatus Magneto-bacterium casensis" (38), similar to MTB recovered in other freshwater sediments from Germany, China, and Russia, etc. (18,25,39,40). The sediment was transferred to centrifuge tubes, 4,800 l distilled water was then added to 200 l of sediment (5 ml total), and the mixture was homogenized by shaking.…”

Section: Methodsmentioning

confidence: 99%

Constant Flux of Spatial Niche Partitioning through High-Resolution Sampling of Magnetotactic Bacteria

Gilder

Orsi

et al. 2017

Appl Environ Microbiol

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Magnetotactic bacteria (MTB) swim along magnetic field lines in water. They are found in aquatic habitats throughout the world, yet knowledge of their spatial and temporal distribution remains limited. To help remedy this, we took MTB-bearing sediment from a natural pond, mixed the thoroughly homogenized sediment into two replicate aquaria, and then counted three dominant MTB morphotypes (coccus, spirillum, and rod-shaped MTB cells) at a high spatiotemporal sampling resolution: 36 discrete points in replicate aquaria were sampled every ϳ30 days over 198 days. Population centers of the MTB coccus and MTB spirillum morphotypes moved in continual flux, yet they consistently inhabited separate locations, displaying significant anticorrelation. Rod-shaped MTB were initially concentrated toward the northern end of the aquaria, but at the end of the experiment, they were most densely populated toward the south. The finding that the total number of MTB cells increased over time during the experiment argues that population reorganization arose from relative changes in cell division and death and not from migration.The maximum net growth rates were 10, 3, and 1 doublings day Ϫ1 and average net growth rates were 0.24, 0.11, and 0.02 doublings day Ϫ1 for MTB cocci, MTB spirilla, and rod-shaped MTB, respectively; minimum growth rates for all three morphotypes were Ϫ0.03 doublings day Ϫ1 . Our results suggest that MTB cocci and MTB spirilla occupy distinctly different niches: their horizontal positioning in sediment is anticorrelated and under constant flux.IMPORTANCE Little is known about the horizontal distribution of magnetotactic bacteria in sediment or how the distribution changes over time. We therefore measured three dominant magnetotactic bacterium morphotypes at 36 places in two replicate aquaria each month for 7 months. We found that the spatial positioning of population centers changed over time and that the two most abundant morphotypes (MTB cocci and MTB spirilla) occupied distinctly different niches in the aquaria. Maximum and average growth and death rates were quantified for each of the three morphotypes based on 72 sites that were measured six times. The findings provided novel insight into the differential behavior of noncultured magnetotactic bacteria.KEYWORDS magnetotactic bacteria, spatiotemporal variation, controlled aquaria A lmost all freshwater and marine environments host a broad spectrum of special kinds of bacteria, called magnetotactic bacteria (MTB) (1), that synthesize membrane-bound, single-domain-sized (35-to 120-nm) magnetite and/or greigite crystals called magnetosomes (2-4). Chains of magnetosomes that contain permanent magnetic moments are fixed within the cell, which enable MTB to swim along magnetic field lines (5, 6). MTB host a wide variety of cell morphotypes, including cocci, vibrios, spirilla, ovoids, rods, and multicellular prokaryotes (7).

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Section: Methodsmentioning

confidence: 99%

Constant Flux of Spatial Niche Partitioning through High-Resolution Sampling of Magnetotactic Bacteria

Gilder

Orsi

et al. 2017

Appl Environ Microbiol

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“…Here, we present the draft genome sequence of Magnetospirillum sp. strain SO-1, a freshwater magnetotactic spirillum isolated from the sediments of the Ol’khovka River (43°56′07″N, 42°41′25″E; Caucasus region, Russia) (3). …”

Section: Genome Announcementmentioning

confidence: 99%

Draft Genome Sequence of Magnetospirillum sp. Strain SO-1, a Freshwater Magnetotactic Bacterium Isolated from the Ol'khovka River, Russia

et al. 2014

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“…The magnetosome arrangement within the cells varies from strain to strain: they are organized in one, two, or more chains and can be assembled as a dispersed cluster (Pan et al, 2008; Lefèvre et al, 2009; Lin and Pan, 2009; Abreu et al, 2012; Zhang et al, 2012, 2017; Morillo et al, 2014; Du et al, 2017; Kozyaeva et al, 2017). Many magnetotactic cocci have sulfur globules, indicating their ability to use reduced sulfur compounds, and phosphorus-rich inclusions that sometimes occupy most of the cell volume (Cox et al, 2002; Dziuba et al, 2013; Zhang et al, 2017). Cultivated magnetotactic cocci are microaerophiles and use thiosulfate for autotrophic growth, while using acetate and, in the case of IT-1, succinate for heterotrophic growth (Williams et al, 2006; Lefèvre et al, 2009; Morillo et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Genome-Based Metabolic Reconstruction of a Novel Uncultivated Freshwater Magnetotactic coccus “Ca. Magnetaquicoccus inordinatus” UR-1, and Proposal of a Candidate Family “Ca. Magnetaquicoccaceae”

et al. 2019

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Magnetotactic bacteria are widely represented microorganisms that have the ability to synthesize magnetosomes. The magnetotactic cocci of the order Magnetococcales are the most frequently identified, but their classification remains unclear due to the low number of cultivated representatives. This paper reports the analysis of an uncultivated magnetotactic coccus UR-1 collected from the Uda River (in eastern Siberia). Genome analyses of this bacterium and comparison to the available Magnetococcales genomes identified a novel species called “Ca. Magnetaquicoccus inordinatus,” and a delineated candidate family “Ca. Magnetaquicoccaceae” within the order Magnetococcales is proposed. We used average amino acid identity values <55–56% and <64–65% as thresholds for the separation of families and genera, respectively, within the order Magnetococcales. Analyses of the genome sequence of UR-1 revealed a potential ability for a chemolithoautotrophic lifestyle, with the oxidation of a reduced sulfur compound and carbon assimilation by rTCA. A nearly complete magnetosome genome island, containing a set of mam and mms genes, was also identified. Further comparative analyses of the magnetosome genes showed vertical inheritance as well as horizontal gene transfer as the evolutionary drivers of magnetosome biomineralization genes in strains of the order Magnetococcales.

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Species diversity of magnetotactic bacteria from the Ol’khovka River, Russia

Cited by 13 publications

References 19 publications

Constant Flux of Spatial Niche Partitioning through High-Resolution Sampling of Magnetotactic Bacteria

Constant Flux of Spatial Niche Partitioning through High-Resolution Sampling of Magnetotactic Bacteria

Draft Genome Sequence of Magnetospirillum sp. Strain SO-1, a Freshwater Magnetotactic Bacterium Isolated from the Ol'khovka River, Russia

Genome-Based Metabolic Reconstruction of a Novel Uncultivated Freshwater Magnetotactic coccus “Ca. Magnetaquicoccus inordinatus” UR-1, and Proposal of a Candidate Family “Ca. Magnetaquicoccaceae”

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