Species diversity in EuropeanHaematococcus pluvialis(Chlorophyceae, Volvocales)

Allewaert, Céline C.; Vanormelingen, Pieter; Pröschold, Thomas; Gómez, Patricia; González, Mariela A.; Bilcke, Gust; D’hondt, Sofie; Vyverman, Wim

doi:10.2216/15-55.1

Cited by 37 publications

(32 citation statements)

References 79 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Allewaert et al. () in their recent studies on European Haematococcus species observed high intraspecific variation in optimal temperature and maximum growth rate.…”

Section: Resultsmentioning

confidence: 96%

See 1 more Smart Citation

Effect of culturing parameters on the vegetative growth of Haematococcus alpinus (strain lcr‐cc‐261f) and modeling of its growth kinetics

Mazumdar

Novis

Visnovsky

et al. 2019

Journal of Phycology

View full text Add to dashboard Cite

The present study investigated the effect of different culture conditions on the vegetative growth of a new species, Haematococcus alpinus (strain LCR‐CC‐261f) using airlift photobioreactors. The influence of culture medium, aeration rates, CO2 concentration in air‐gas mixture, temperature, light intensities, and wavelengths were investigated to achieve sustainable high cell density cultures. Growth parameters were determined by fitting the data to a form of the logistic equation that included a lag phase. The shear‐sensitive vegetative cells favored lower aeration rates in the photobioreactors. MLA medium increased to 40 mM nitrate produced high density cultures. Temperatures between 12°C and 18°C, 3% (v/v) CO2 concentration and a narrow photon flux density ranging between 37 and 48 μmol photons · m−2 · s−1 were best suited for growth. The wavelength of the light source also impacted growth and a high cell density of 9.6 × 105 cells · mL−1 was achieved using a mixture of red and blue compared to warm white, red, or blue LEDs.

show abstract

“…Allewaert et al. () in their recent studies on European Haematococcus species observed high intraspecific variation in optimal temperature and maximum growth rate.…”

Section: Resultsmentioning

confidence: 96%

“…Klochkova et al (2013) reported a cold tolerant strain of H. lacustris capable of growing at 4°C-10°C. Allewaert et al (2015) in their recent studies on European Haematococcus species observed high intraspecific variation in optimal temperature and maximum growth rate.…”

Section: Resultsmentioning

confidence: 96%

Effect of culturing parameters on the vegetative growth of Haematococcus alpinus (strain lcr‐cc‐261f) and modeling of its growth kinetics

Mazumdar

Novis

Visnovsky

et al. 2019

Journal of Phycology

View full text Add to dashboard Cite

show abstract

“…pluvialis or “A” lineage [37]). Nucleotide alignments of ITS2 data for these analyses were guided by secondary structure as implemented in 4Sale v 1.7 [39, 40] (see also below).…”

Section: Methodsmentioning

confidence: 99%

Assessing intragenomic variation of the internal transcribed spacer two: Adapting the Illumina metagenomics protocol

Alanagreh¹,

Pegg²,

Harikumar³

et al. 2017

PLoS ONE

View full text Add to dashboard Cite

Primary and secondary structural data from the internal transcribed spacer two (ITS2) have been used extensively for diversity studies of many different eukaryotic organisms, including the green algae. Ease of amplification is due, at least in part, to the fact that ITS2 is part of the tandemly-repeated rRNA array. The potential confounding influence of intragenomic variability has yet to be addressed except in a few organisms. Moreover, few of the assessments of intragenomic variation have taken advantage of the deep sequencing capacity of sequence-by-synthesis protocols. We present results from our adaptation of the 16S Metagenomics Sequencing Library Preparation/Illumina protocol for deep sequencing of the ITS2 genes in selected isolates of the green algal genus, Haematococcus. Deep sequencing yielded from just under 20,000 to more than 500,000 merged reads, outpacing results from recent pyrosequencing efforts. Furthermore, a conservative evaluation of these data revealed a range of three to six ITS2 sequence haplotypes (defined as unique sets of nucleotide polymorphisms) across the taxon sampling. The frequency of the dominant haplotype ranged from 0.35 to 0.98. In all but two cases, the haplotype with the greatest frequency corresponded to a sequence obtained by the Sanger method using PCR templates. Our data also show that results from the sequencing-by-synthesis approach are reproducible. In addition to advancing our understanding of ribosomal RNA variation, the results of this investigation will allow us to begin testing hypotheses regarding the maintenance of homogeneity across multi-copy genes.

show abstract

“…Occasionally, modern phycologists have attempted to revisit type localities and establish new types for old species and genus names that would otherwise be taxonomically questionable or ambiguous (e.g., Fučíková et al 2013). In some cases, an existing isolate is selected to serve as new type, ideally one collected near the type locality (e.g., Allewaert et al 2015)-often this is the most practical solution, especially when the locality information is insufficient in the original species description, and it is thus impossible to find and revisit it. The description of the type locality of Dispora speciosa (North part of the European part of the former USSR (Korshikov 1953)) is very broad and thus collecting material from the original site is not possible.…”

Section: Introductionmentioning

confidence: 99%

Dispora speciosa, a new addition to the genus Parallela and the first coccoid member of the family Microsporaceae

Štenclová¹,

Fučíková²

2019

Phytotaxa

View full text Add to dashboard Cite

The clade that currently represents the green algal family Microsporaceae is one of the few filament-forming groups of Chlorophyceae. Molecular phylogenies show this clade containing the genus Microspora and the more recently circumscribed Parallela, whose filaments are loosely arranged and often multiseriate. We initially investigated the enigmatic bog-loving Dispora speciosa as a commonly accepted member of the mucilage-forming Radiococcaceae or a putative member of crucigenoid chlorophytes (a non-monophyletic group formerly placed in Scenedesmaceae) based on its two-dimensional colony formation. However, our plastid and nuclear ribosomal phylogenies confidently placed Dispora within the genus Parallela instead, and therefore distantly related to both Radiococcaceae and crucigenoids. Upon further examination of the cell morphology and ultrastructure, we found several corresponding features between Dispora and Parallela, despite Dispora’s apparent coccoid-colonial gross morphology. Both genera have cells with a parietal plastid positioned around a large central nucleus. The loose, multiseriate filament formation in Parallela can be interpreted as similar to Dispora’s flat colony formation in its natural state. Because we only present data from one non-type species and strain of Dispora, we cannot merge the entire genus with Parallela. We do however argue that D. speciosa, of which this strain is the sole available, morphologically and ecologically faithful representative, should be transferred into Parallela, and the specimen prepared from strain ACOI 1508 be designated as type. Our study also impacts the current view on evolution of multicellular (colonial and filamentous) forms in Chlorophyceae.

show abstract

Species diversity in EuropeanHaematococcus pluvialis(Chlorophyceae, Volvocales)

Cited by 37 publications

References 79 publications

Effect of culturing parameters on the vegetative growth of Haematococcus alpinus (strain lcr‐cc‐261f) and modeling of its growth kinetics

Effect of culturing parameters on the vegetative growth of Haematococcus alpinus (strain lcr‐cc‐261f) and modeling of its growth kinetics

Assessing intragenomic variation of the internal transcribed spacer two: Adapting the Illumina metagenomics protocol

<p><strong><em>Dispora speciosa</em></strong><strong>, a new addition to the genus <em>Parallela</em> and the first coccoid member of the family Microsporaceae</strong></p>

Contact Info

Product

Resources

About