Species Distribution and Isolation Frequency of Nontuberculous Mycobacteria, Uruguay

Greif, Gonzalo; Coitinho, Cecilia; Ingen, Jakko van; Robello, Carlos

doi:10.3201/eid2605.191631

Cited by 7 publications

(3 citation statements)

References 14 publications

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“…Total 39 different NTM species were identified, indicating the diversity of NTM species circulating among patients in China. M. intracellulare was the most common NTM pathogen, which was similar to previous findings in Australia, Korea, India, Uruguay and so on ( 3 , 16 – 19 ). Furthermore, we found the incidence of M. avium grew faster than others from 2014 to 2021, and the ratio of M. avium to M. intracellulare increased each year, suggesting a more prominent role for M. avium infections in China.…”

Section: Discussionsupporting

confidence: 89%

Trends and Species Diversity of Non-tuberculous Mycobacteria Isolated From Respiratory Samples in Northern China, 2014–2021

Sun

Yan

Liao

et al. 2022

Front. Public Health

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BackgroundPulmonary non-tuberculous mycobacteria (NTM) infection has become a public health concern in China and around the world. The objective of this study was to describe the longitudinal changes in the frequency and diversity of NTM in northern China.MethodsWe retrospectively analyzed data on mycobacterium species in Beijing Chest Hospital from January 2014 to December 2021. The isolates were identified to species level by targeted DNA sequencing.ResultsAfter excluding duplicates, 1,755 NTM strains were analyzed, which were from 27 provinces in China over 8 years. Among all mycobacteria, the proportion of NTM increased each year, from 4.24% in 2014 to 12.68% in 2021. Overall, 39 different NTM species were identified, including 23 slow growing mycobacteria (SGM) and 16 rapid growing mycobacteria (RGM). The most common species were M. intracellulare (51.62%), M. abscessus (22.22%), M. kansasii (8.32%), M. avium (7.75%) and M. fortuitum (2.05%). The number of NTM species identified also increased each year from 9 in 2014 to 26 in 2021. Most species showed stable isolation rates over the years; however, the proportion of M. avium increased from 3.85 to 10.42% during the study period. Besides, 81 non-mycobacteria strains, including Gordonia (21 isolates), Nocardia (19 isolates) and Tsukamurella (17 isolates), etc., were also discovered.ConclusionThe proportion of NTM and species diversity increased considerably in northern China from 2014 to 2021. M. intracellulare was the most common NTM isolated among respiratory specimens, followed by M. abscessus and M. kansasii. Rare NTM species and non-mycobacteria pathogens also need attention.

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Section: Discussionsupporting

confidence: 89%

Trends and Species Diversity of Non-tuberculous Mycobacteria Isolated From Respiratory Samples in Northern China, 2014–2021

Sun

Yan

Liao

et al. 2022

Front. Public Health

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“…These species were all recovered from environmental samples on RGM medium during this study. Additionally, a study of pulmonary and extrapulmonary samples from patients also identified M. gordonae and M. avium frequently, as well as M. intracellulare, M. kansasii, and M. peregrinum [28]. During our study nine NTM species from the CDC list of mycobacteria causing HAIs [7] were isolated on RGM medium from environmental samples.…”

Section: Plos Onementioning

confidence: 61%

“…kansasii , and M . peregrinum [ 28 ]. During our study nine NTM species from the CDC list of mycobacteria causing HAIs [ 7 ] were isolated on RGM medium from environmental samples.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of a new culture medium for isolation of nontuberculous mycobacteria from environmental water samples

Alexander¹,

Furlong²,

Baron³

et al. 2021

PLoS ONE

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Nontuberculous mycobacteria (NTM) are waterborne pathogens commonly found in building water systems where they are a primary concern to vulnerable patient populations and can cause severe disease. The recovery of NTM from environmental samples can be a laborious undertaking and current pre-treatment methods and selective media lack sensitivity. We explored the use of the highly selective Rapidly Growing Mycobacteria (RGM) medium for culturing NTM from environmental water samples compared to existing methods. In total, 223 environmental water samples, including potable and non-potable water, were cultured for NTM using three culture media. In addition to direct culture on RGM medium, each sample was cultured on Middlebrook 7H10 medium and Mitchison 7H11 medium after pre-treatment with 0.2M KCl-HCl. Additionally, 33 distinct species of NTM were inoculated onto RGM medium and 7H10 medium in parallel to directly compare their growth. The use of RGM medium alone without pre-treatment provided a sensitivity (91%) comparable to that offered by culture on both 7H10 and 7H11 with acid pretreatment (combined sensitivity; 86%) with significantly less overgrowth and interference from other organisms on RGM medium. The average concentration of NTM observed on RGM medium alone was comparable to or greater than the NTM concentration on either medium alone or combined. Thirty-three species were examined in parallel and all tested strains of 27 of these species successfully grew on RGM medium, including 19 of 21 from the CDC’s healthcare-associated infections species list. RGM medium was successful at recovering environmental NTM without a pre-treatment, greatly reducing labor and materials required to process samples. Simplification of culture processing for environmental NTM will allow for a better assessment of their presence in building water systems and the potential for reduced exposure of susceptible populations.

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Global Epidemiology of Nontuberculous Mycobacterial Pulmonary Disease

Prevots,

Marshall,

Wagner

et al. 2023

Clinics in Chest Medicine

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Species Distribution and Isolation Frequency of Nontuberculous Mycobacteria, Uruguay

Cited by 7 publications

References 14 publications

Trends and Species Diversity of Non-tuberculous Mycobacteria Isolated From Respiratory Samples in Northern China, 2014–2021

Trends and Species Diversity of Non-tuberculous Mycobacteria Isolated From Respiratory Samples in Northern China, 2014–2021

Evaluation of a new culture medium for isolation of nontuberculous mycobacteria from environmental water samples

Global Epidemiology of Nontuberculous Mycobacterial Pulmonary Disease

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