Species Distribution and In Vitro Antifungal Susceptibility Patterns of 75 Clinical Isolates of
            <i>Fusarium</i>
            spp. from Northern Italy

Tortorano, Anna Maria; Prigitano, Anna; Dho, Giovanna; Esposto, Maria Carmela; Gianni, C.; Grancini, Anna; Ossi, C.; Viviani, Maria Anna

doi:10.1128/aac.00272-08

Cited by 76 publications

(71 citation statements)

References 14 publications

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“…(3,19). As in our study, others have reported the FSSC to be the most resistant members of the genus (37). Finally, studies are needed to evaluate whether and to what extent species-and strain-specific differences in antifungal susceptibility exist within Fusarium.…”

Section: Vol 49 2011 Seven-locus Sequence Analysis Of Fusarium 1895supporting

confidence: 55%

Accurate and Practical Identification of 20 Fusarium Species by Seven-Locus Sequence Analysis and Reverse Line Blot Hybridization, and an In Vitro Antifungal Susceptibility Study

Xiao

Kong

et al. 2011

J Clin Microbiol

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Eleven reference and 25 clinical isolates of Fusarium were subject to multilocus DNA sequence analysis to determine the species and haplotypes of the fusarial isolates from Beijing and Shandong, China. Seven loci were analyzed: the translation elongation factor 1 alpha gene (EF-1␣); the nuclear rRNA internal transcribed spacer (ITS), large subunit (LSU), and intergenic spacer (IGS) regions; the second largest subunit of the RNA polymerase gene (RPB2); the calmodulin gene (CAM); and the mitochondrial small subunit (mtSSU) rRNA gene. We also evaluated an IGS-targeted PCR/reverse line blot (RLB) assay for species/haplotype identification of Fusarium. Twenty Fusarium species and seven species complexes were identified. Of 25 clinical isolates (10 species), the Gibberella (Fusarium) fujikuroi species complex was the commonest (40%) and was followed by the Fusarium solani species complex (FSSC) (36%) and the F. incarnatum-F. equiseti species complex (12%). Six FSSC isolates were identified to the species level as FSSC-3؉4, and three as FSSC-5. Twenty-nine IGS, 27 EF-1␣, 26 RPB2, 24 CAM, 18 ITS, 19 LSU, and 18 mtSSU haplotypes were identified; 29 were unique, and haplotypes for 24 clinical strains were novel. By parsimony informative character analysis, the IGS locus was the most phylogenetically informative, and the rRNA gene regions were the least. Results by RLB were concordant with multilocus sequence analysis for all isolates. Amphotericin B was the most active drug against all species. Voriconazole MICs were high (>8 g/ml) for 15 (42%) isolates, including FSSC. Analysis of larger numbers of isolates is required to determine the clinical utility of the seven-locus sequence analysis and RLB assay in species classification of fusaria.

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Section: Vol 49 2011 Seven-locus Sequence Analysis Of Fusarium 1895supporting

confidence: 55%

Accurate and Practical Identification of 20 Fusarium Species by Seven-Locus Sequence Analysis and Reverse Line Blot Hybridization, and an In Vitro Antifungal Susceptibility Study

Xiao

Kong

et al. 2011

J Clin Microbiol

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“…To this regard, as reviewed by Jain et al (2011), up to now 15 species of Fusarium have been reported as etiological agents of infection, always originated from animal or plant source in nature. In Italy, the number of disseminated infections has greatly increased and F. oxysporum and F. verticillioides, species detected in our survey, result among the most prevalent species involved in human mycoses (Tortorano et al 2008).…”

Section: Discussionmentioning

confidence: 73%

A model of co-occurrence: segregation and aggregation patterns in the mycoflora of the crayfish Procambarus clarkii in Lake Trasimeno (central Italy)

et al. 2012

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“…Most of the species have shown in vitro resistance to azoles, polyenes, and echinocandins; numerous experimental studies in animals carried out to evaluate different antifungal treatments have had disappointing results, and a favorable outcome was found in less than 50% of the cases (5,7,9,14,15,19).…”

mentioning

confidence: 99%

Cross-Reactivity of Fusarium spp. in the Aspergillus Galactomannan Enzyme-Linked Immunosorbent Assay

et al. 2012

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Nine of 11 hematological patients with disseminated/deep-seated Fusarium infection tested at least twice for Aspergillus galactomannan (GM) had repeated positive results in the absence of Aspergillus isolation in culture. The centrifuged supernatants of 12 Fusarium isolates were tested by a GM enzyme-linked immunosorbent assay (EIA). All the isolates produced positive reactions when tested undiluted. These results show cross-reactivity of Fusarium spp. with Aspergillus GM that may constitute a drawback with respect to the specificity of the Platelia EIA.

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Species Distribution and In Vitro Antifungal Susceptibility Patterns of 75 Clinical Isolates of Fusarium spp. from Northern Italy

Cited by 76 publications

References 14 publications

Accurate and Practical Identification of 20 Fusarium Species by Seven-Locus Sequence Analysis and Reverse Line Blot Hybridization, and an In Vitro Antifungal Susceptibility Study

Accurate and Practical Identification of 20 Fusarium Species by Seven-Locus Sequence Analysis and Reverse Line Blot Hybridization, and an In Vitro Antifungal Susceptibility Study

A model of co-occurrence: segregation and aggregation patterns in the mycoflora of the crayfish Procambarus clarkii in Lake Trasimeno (central Italy)

Cross-Reactivity of Fusarium spp. in the Aspergillus Galactomannan Enzyme-Linked Immunosorbent Assay

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