Species Distribution and Characterization Studies of Sex-Limited Esterases within the virgifera Group of Diabrotica (Coleoptera: Chrysomelidae)

Ruud, Susan; McDonald, Ian C.; Gabrielson, David A.; Johnson, O. A.

doi:10.1093/aesa/81.6.954

Cited by 2 publications

(1 citation statement)

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“…The 29-kDa antibody recognized two lower molecular weight resistanceassociated proteins (20-and 29-kDa) but only in male beetles. The existence of a male-linked esterase (Est-m) in D. v. virgifera (Ruud et al, 1988;McDonald et al, 1990) with a molecular weight of approximately 78-kDa and its activity localized in the abdomens of virgin males has been previously documented. The two male-linked proteins identified in the present study are much smaller than Est-m, although Rudd et al (1988) suggested the existence of other sex-linked loci in both D. v. virgifera and D. barberi.…”

Section: Discussionmentioning

confidence: 98%

Immunological assessment of an insecticide resistance-associated esterase in the Western corn rootworm

Zhou

Scharf

Meinke

et al. 2005

Arch. Insect Biochem. Physiol.

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In previous investigations, we have determined that organophosphate resistance in the western corn rootworm, Diabrotica virgifera virgifera, is at least partially attributed to a group of non-specific carboxylesterases referred to as group II. Antiserum raised against a purified 66-kDa group II esterase is specific for the denatured enzyme. This antiserum reacts similarly with both beetle homogenates from resistant and susceptible populations, although there is much higher signal intensity in immunoblots of resistant relative to susceptible beetles. These results suggest that overproduction of group II esterases is the underlying basis of esterase-mediated resistance in D. v. virgifera by demonstrating that (1) group II esterases are immunologically indistinguishable between the resistant and susceptible populations, and (2) the intensity differences are due to increased group II esterase proteins in the resistant population. The diagnostic potential of immunological-based assays was tested with a traditional diagnostic concentration bioassay and a biochemical-based native PAGE assay. Significant correlations were observed among all three diagnostic assays (regression coefficients ranging from 0.95 to 0.96). These results demonstrate the importance of the 66-kDa protein as a resistance-associated biochemical marker, thus emphasizing the potential for 66-kDa protein-targeted immunoassays in resistance monitoring programs. Arch. Insect Biochem. Physiol. 58:157-165, 2005.

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Section: Discussionmentioning

confidence: 98%