Species distribution and antimicrobial susceptibility of staphylococci isolated from canine otitis externa

Abstract

The diversity of species of the genus Staphylococcus sp. and the antimicrobial resistance of isolates from 151 unmedicated dogs of both sexes with a clinical diagnosis of otitis were recorded. Ninety-one isolates of Staphylococcus spp. were identified by biochemical reactions and tested for susceptibility to 15 antimicrobials. Coagulase-positive species were most common; S. pseudintermedius (38.4%), S. schleiferi schleiferi (15.4%), S. aureus (14.3%), S. epidermidis (11%), S. simulans (11%), S. schleiferi coagulans (8.8%) and S. saprophyticus (1.1%). All the isolates showed resistance to at least one drug and 89% were multiresistant. Amoxicillin combined with clavulanic acid and oxacillin were the most effective, while resistance was widely observed for neomycin and erythromycin. The results highlight the recognition and the potential need for bacterial culture with species identification and antimicrobial susceptibility tests for appropriate antimicrobial therapy.

Frequency and antimicrobial susceptibility of Staphylococcus pseudintermedius in dogs with otitis externa

“…pseudintermedius was detected in almost 60% of dogs with otitis externa. This rate is higher than that reported in previous studies (LILENBAUM et al, 2000;HOEKSTRA & PAULTON, 2002;PENNA et al, 2010;SAPUTRA et al, 2017). This variation in results might be due to geographical differences, and clinical conditions (with and/or without otitis), age, and breed of the dogs.…”

“…In Brazil, studies examining otitis caused by S. pseudintermedius in dogs are scarce, making any comparison difficult. The isolation rate obtained in this study was greater than that reported by the two previously published studies in Brazil (LILENBAUM et al, 2000;PENNA et al, 2010). Taken together, our results indicated that S. pseudintermedius is an important bacterium causing otitis in dogs and should be considered when treating this disease.…”

“…One study in Brazil reported that more than 90% of staphylococci strains were sensitive to tetracycline (LILENBAUM et al, 2000). Reports of enrofloxacin resistance described similar results (PENNA et al, 2010;DE MARTINO et al, 2016), while in Europe the level of susceptibility to fluoroquinolones has been reported as over 90% (KROEMER et al, 2014).…”

“…In contrast, less than 23% of the strains were susceptible to penicillin. High susceptibility rates to amoxicillin plus clavulanic have been reported previously (PENNA et al, 2010;KROEMER et al, 2014;DE MARTINO et al, 2016). However, resistance to penicillin has not been previously reported ( LILENBAUM et al, 2000, KROEMER et al, 2014;DE MARTINO et al, 2016).…”

“…Aminoglycoside resistance was observed in 27.3 of strains for gentamicin. Previous studies have shown that resistance to aminoglycosides is commonly high (PENNA et al, 2010;DE MARTINO et al, 2016).…”