Species distribution and antifungal susceptibility profile of Candida isolates from blood and other normally sterile foci from pediatric ICU patients in Tehran, Iran

Mirhendi, Hossein; Charsizadeh, Arezoo; Eshaghi, Hamid; Nikmanesh, Bahram; Arendrup, Maiken Cavling

doi:10.1093/mmy/myz047

Cited by 13 publications

(21 citation statements)

References 32 publications

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“…In this study we compared the performance of Vitek 2 and 21-plex PCR for yeast species identification in an epidemiological study of yeast BSIs in Iran. In our epidemiological study, C. albicans was the most prevalent Candida species isolated; this result is consistent with the findings of a previous studies conducted in paediatric patients in Tehran [ 7 , 26 ]. However, we found that C. glabrata was the second most prevalent Candida species, followed by C. parapsilosis, while Mirhendi et al found that C. parapsilosis was the second most prevalent cause of candidaemia [ 26 ].…”

Section: Discussionsupporting

confidence: 93%

“…In our epidemiological study, C. albicans was the most prevalent Candida species isolated; this result is consistent with the findings of a previous studies conducted in paediatric patients in Tehran [ 7 , 26 ]. However, we found that C. glabrata was the second most prevalent Candida species, followed by C. parapsilosis, while Mirhendi et al found that C. parapsilosis was the second most prevalent cause of candidaemia [ 26 ]. This is because they studied neonates and children, who are more prone to developing candidaemia due to C. parapsilosis [ 27 ].…”

Section: Discussionsupporting

confidence: 93%

“…Therefore, for laboratories lacking automated Vitek 2, MALDI-TOF, or biochemical identification assays, 21-plex PCR may be a useful technique for the identification of yeasts isolated from clinical samples. In addition, a previous Iranian study assessing candidaemia in paediatric patients showed that 95.7 % of the species identified were in the target list of the 21-plex PCR [ 26 ]. Hence, 21-plex PCR can be used as a useful standalone technique in the routine laboratories of developing countries lacking specific and accurate identification tools, i.e.…”

Section: Discussionmentioning

confidence: 99%

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Epidemiology of yeast species causing bloodstream infection in Tehran, Iran (2015–2017); superiority of 21-plex PCR over the Vitek 2 system for yeast identification

Kord

Salehi

Khodavaisy

et al. 2020

Journal of Medical Microbiology

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Introduction. Given the limited number of candidaemia studies in Iran, the profile of yeast species causing bloodstream infections (BSIs), especially in adults, remains limited. Although biochemical assays are widely used in developing countries, they produce erroneous results, especially for rare yeast species. Aim. We aimed to assess the profile of yeast species causing BSIs and to compare the accuracy of the Vitek 2 system and 21-plex PCR. Methodology. Yeast blood isolates were retrospectively collected from patients recruited from two tertiary care training hospitals in Tehran from 2015 to 2017. Relevant clinical data were mined. Identification was performed by automated Vitek 2, 21-plex PCR and sequencing of the internal transcribed spacer region (ITS1-5.8S-ITS2). Results. In total, 137 yeast isolates were recovered from 107 patients. The overall all-cause 30-day mortality rate was 47.7 %. Fluconazole was the most widely used systemic antifungal. Candida albicans (58/137, 42.3 %), Candida glabrata (30/137, 21.9 %), Candida parapsilosis sensu stricto (23/137, 16.8 %), Candida tropicalis (10/137, 7.3 %) and Pichia kudriavzevii ( Candida krusei ) (4/137, 2.9 %) constituted almost 90 % of the isolates and 10 % of the species detected were rare yeast species (12/137; 8.7 %). The 21-plex PCR method correctly identified 97.1 % of the isolates, a higher percentage than the Vitek 2 showed (87.6 %). Conclusion. C. albicans was the main cause of yeast-derived fungaemia in this study. Future prospective studies are warranted to closely monitor the epidemiological landscape of yeast species causing BSIs in Iran. The superiority of 21-plex PCR over automated Vitek 2 indicates its potential clinical utility as an alternative identification tool use in developing countries.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

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Epidemiology of yeast species causing bloodstream infection in Tehran, Iran (2015–2017); superiority of 21-plex PCR over the Vitek 2 system for yeast identification

Kord

Salehi

Khodavaisy

et al. 2020

Journal of Medical Microbiology

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“…Candida is the most common cause of digestive tract infection, and Aspergillus is an invasive lung infection caused by Aspergillus (1). Moreover, in recent years, new species of Aspergillus are reported; however, their toxicology, drug resistance, and growth conditions have not been fully defined.…”

Section: Discussionmentioning

confidence: 99%

“…The incidence of opportunistic fungal infections is on the rise, mainly due to population aging and enhanced incidence of malignant tumors. Candida is the most common cause of digestive tract infection, while Aspergillus is known as the most likely cause of invasive lung infection (1). In China, the mortality of untreated pulmonary Aspergillus infection is high (2)(3)(4), and Aspergillus fumigatus infections account for 70% -80% of pulmonary Aspergillus infections (5).…”

Section: Introductionmentioning

confidence: 99%

The Effects of Different Fungi on the IL-1β Expression in Mouse Dendritic Cells

Zhang

Wang

Wan

et al. 2020

Jundishapur J Microbiol

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Background: Invasive fungal infection received more and more attention because of its high mortality, Candida albicans and Aspergillus fumigatus is the most common pathogenic fungus for systematic fungal infection, A. lentulus was isolated and identified recently and named as a sister of A. fumigatus. Objectives: The current study aimed to explore the concentration and time-dependent relationships of the virulence of fungi due to the change in the Interleukin-1 beta (IL-1β) level. Methods: Candida albicans, A. fumigatus, and A. lentulus suspensions with a multiplicity of infection = 0, 1, 5, 10, and 20 units were used to treat mouse dendritic cells. The IL-β level was measured by enzyme-linked immunosorbent assay (ELISA) at 2, 6, 12, 24, and 48 hours after the treatment was administered. Results: The main effects and interactions between the multiplicity of infection, intervention duration, and the dependent variable of IL-1β were significant. Besides, there were statistically significant differences. Only C. albicans and A. lentulus could induce IL-1β 48 hours after administration. Furthermore, the production of IL-1β induced by A. fumigatus was higher than that induced by A. lentulus and C. albicans. Conclusions: This study demonstrated concentration- and time-dependent relationships in IL-1β production by dendritic cells induced by three types of fungi. Candida albicans and A. lentulus exhibited a slow phase-in in vitro inflammation induction. The inflammatory response induced by A. fumigatusin vitro has the characteristics of a short action time and a strong toxic effect. Finally, A. lentulus is less virulent than A. fumigatus, and its inflammation-inducing time is relatively longer.

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Multiplex size marker (YEAST PLEX) for rapid and accurate identification of pathogenic yeasts

Aboutalebian

Mahmoudi

Charsizadeh

et al. 2022

Clinical Laboratory Analysis

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Species distribution and antifungal susceptibility profile of Candida isolates from blood and other normally sterile foci from pediatric ICU patients in Tehran, Iran

Cited by 13 publications

References 32 publications

Epidemiology of yeast species causing bloodstream infection in Tehran, Iran (2015–2017); superiority of 21-plex PCR over the Vitek 2 system for yeast identification

Epidemiology of yeast species causing bloodstream infection in Tehran, Iran (2015–2017); superiority of 21-plex PCR over the Vitek 2 system for yeast identification

The Effects of Different Fungi on the IL-1β Expression in Mouse Dendritic Cells

Multiplex size marker (YEAST PLEX) for rapid and accurate identification of pathogenic yeasts

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