Species Differentiation of Oral Lactobacilli From Man Including Descriptions of Lactobacillus Salivarius Nov Spec and Lactobacillus Cellobiosus Nov Spec

Rogosa, Morrison; Wiseman, R. F.; Mitchell, Joyce A.; Disraely, M.N.; Beaman, Alfred J.

doi:10.1128/jb.65.6.681-699.1953

Cited by 191 publications

(70 citation statements)

References 25 publications

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“…Although the intensity must be interpreted with caution, taking into consideration possible PCR bias, enumeration of L. sobrius cells by FISH from the same samples showed a significant drop from 7.87 AE 0.44 log cells g À1 digesta at weaning to 5.37 AE 0.34 log cells g À1 digesta one day later (Pieper et al, unpublished data), which is in good agreement with the data reported here. Lactobacilli characteristic for saliva (L. salivarius) (Rogosa et al, 1953) have also been found to be present at this time. The observed higher pH of the ileal contents one day after weaning (Pieper et al, unpublished data) is probably one of the reasons why they may survive as a dominant group.…”

Section: Discussionmentioning

confidence: 67%

Changes in the diversity of pig ileal lactobacilli around weaning determined by means of 16S rRNA gene amplification and denaturing gradient gel electrophoresis

Janczyk

Pieper

Smidt

et al. 2007

FEMS Microbiology Ecology

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Our study aimed to provide a comprehensive characterization of changes in porcine intestinal Lactobacillus populations around the time of weaning based on 16S rRNA gene amplification and denaturing gradient gel electrophoresis (DGGE). DNA was extracted from the ileal contents of piglets at weaning (28 days of age) and after 1, 2, 5 and 11 days. PCR amplicons (V2-V3 fragments of 16S rRNA genes) were separated using DGGE. Predominant bands were excised and sequenced after reamplification. A band corresponding to Lactobacillus salivarius was present 1 and 2 days post-weaning (pw), while Lactobacillus crispatus was detected only 1 and 11 days pw. Lactobacillus sobrius gave the most dominant band in all animals. The number of bands decreased from 13+/-3 at weaning to 9+/-1 at 5 days pw, but the species richness had recovered by 11 days pw. The similarity of profiles between sampling days was high for 1 and 2 days pw (>91%), but was low for 5 and 11 days pw (<59%). The diversity of the profiles was lower 5 days pw, based on the Shannon diversity index (0.83+/-0.076 vs. 1.02+/-0.127 at weaning, P=0.042), but had recovered to preweaning values by 11 days pw. The application of group-specific DGGE showed that the Lactobacillus community within the porcine ileum undergoes dramatic, partly reversible changes as a consequence of weaning.

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Section: Discussionmentioning

confidence: 67%

Changes in the diversity of pig ileal lactobacilli around weaning determined by means of 16S rRNA gene amplification and denaturing gradient gel electrophoresis

Janczyk

Pieper

Smidt

et al. 2007

FEMS Microbiology Ecology

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“…A high similarity in carbohydrate utilization patterns was observed between L. fermentum and L. cellobiosus isolates. These species are the subject of considerable controversy with regard to their taxonomic position and separation [25][26][27], and very recently Dellaglio et al [28] have proposed that L. cellobiosus be reclassified and renamed as L. fermentum.…”

Section: Resultsmentioning

confidence: 99%

Genetic diversity (RAPD-PCR) of lactobacilli isolated from ?Almagro? eggplant fermentations from two seasons

Seseña¹,

Sánchez²,

Palop³

2004

FEMS Microbiology Letters

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Genetic diversity of 323 strains of lactobacilli isolated from an Almagro eggplant manufacturing plant was analyzed by using random amplified polymorphic DNA (RAPD). Thirty-four distinct RAPD patterns were obtained with 95% of isolates grouped into 18 main clusters. Genetic diversity was higher in brines from season II, Lactobacillus plantarum and Lactobacillus fermentum/cellobiosus being the species with the greatest number of genotypes. A single L. fermentum/cellobiosus genotype comprised isolates from both seasons and could be considered endemic to that factory.

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“…Lactobacillus salivarius is part of the indigenous microbiota of the mammalian gastrointestinal tract and was first isolated from the human oral cavity (Rogosa et al 1953). The species has also been isolated from human breast milk (Martin et al 2006), a plausible route for gastrointestinal colonization (Martin et al 2006;Jara et al 2011), and from food and environmental sources (Neville and O'Toole 2010).…”

Section: Discussionmentioning

confidence: 99%

“…reuteri ATCC 55730 and Lact. salivarius ATCC 11741, references established as probiotic strains (Rogosa et al 1953;Valeur et al 2004), also inhibited these enteric pathogens. Other marine mammal-derived isolates tested included Staphylococcus sp.…”

Section: Isolation and Identification Of Candidate Probioticsmentioning

confidence: 97%

“…An inhibition radius <4 mm was considered noninhibitory; a radius between 4 and 7 mm was designed as intermediary inhibitor, and a radius exceeding 7 mm was considered strongly inhibitory. Lactobacillus salivarius ATCC 11741, originally isolated from the human oral cavity (Rogosa et al 1953), and recently sequenced as a reference genome by the NIH Human Microbiome Consortium (http://genome.jgi.doe.gov/HumanMicr/HumanMicr.info. html), was used as a reference strain and positive control in these assays.…”

Section: Pathogen Growth Inhibition Assaysmentioning

confidence: 99%

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Identification of L actobacillus strains with probiotic features from the bottlenose dolphin ( T ursiops truncatus )

et al. 2013

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AimsIn order to develop complementary health management strategies for marine mammals, we used culture-based and culture-independent approaches to identify gastrointestinal lactobacilli of the common bottlenose dolphin, Tursiops truncatus.Methods and ResultsWe screened 307 bacterial isolates from oral and rectal swabs, milk and gastric fluid, collected from 38 dolphins in the U.S. Navy Marine Mammal Program, for potentially beneficial features. We focused our search on lactobacilli and evaluated their ability to modulate TNF secretion by host cells and inhibit growth of pathogens. We recovered Lactobacillus salivarius strains which secreted factors that stimulated TNF production by human monocytoid cells. These Lact. salivarius isolates inhibited growth of selected marine mammal and human bacterial pathogens. In addition, we identified a novel Lactobacillus species by culture and direct sequencing with 96·3% 16S rDNA sequence similarity to Lactobacillus ceti.ConclusionsDolphin-derived Lact. salivarius isolates possess features making them candidate probiotics for clinical studies in marine mammals.Significance and Impact of the StudyThis is the first study to isolate lactobacilli from dolphins, including a novel Lactobacillus species and a new strain of Lact. salivarius, with potential for veterinary probiotic applications. The isolation and identification of novel Lactobacillus spp. and other indigenous microbes from bottlenose dolphins will enable the study of the biology of symbiotic members of the dolphin microbiota and facilitate the understanding of the microbiomes of these unique animals.

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Species Differentiation of Oral Lactobacilli From Man Including Descriptions of Lactobacillus Salivarius Nov Spec and Lactobacillus Cellobiosus Nov Spec

Cited by 191 publications

References 25 publications

Changes in the diversity of pig ileal lactobacilli around weaning determined by means of 16S rRNA gene amplification and denaturing gradient gel electrophoresis

Changes in the diversity of pig ileal lactobacilli around weaning determined by means of 16S rRNA gene amplification and denaturing gradient gel electrophoresis

Genetic diversity (RAPD-PCR) of lactobacilli isolated from ?Almagro? eggplant fermentations from two seasons

Identification of L actobacillus strains with probiotic features from the bottlenose dolphin ( T ursiops truncatus )

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