Abstract:1. The secretion of the adrenal cortex in several mammalian species has been studied by direct analysis of samples of adrenal venous blood, using paper chromatography.
2. All the species examined were found to secrete large amounts of 17-hydroxycorticosterone and/or corticosterone, and some a third compound, provisionally identified as 11-hydroxy- androst-4-ene-3:17-dione.
3. The ratio of 17-hydroxycorticosterone to corticosterone secreted, varied from < 0·05 in rats to > 20 in rhesus monkeys… Show more
“…The method, although not specific for corticosterone, detects this steroid in much lower concentrations than any other naturally occurring corticosteroid. It is therefore a suitable method of assay in the rat since corticosterone is the major corticosteroid secreted by the adrenal glands in this species (Bush, 1953).…”
“…The method, although not specific for corticosterone, detects this steroid in much lower concentrations than any other naturally occurring corticosteroid. It is therefore a suitable method of assay in the rat since corticosterone is the major corticosteroid secreted by the adrenal glands in this species (Bush, 1953).…”
“…Aldosterone is produced by capsular cells only (88). Progesterones are also being produced by the rat adrenal (60), as are androgens (28). corticosterone concentrations, has been demonstrated in the rat (36).…”
Section: B Brain-pituitary-adrenal Interactionsmentioning
“…The assay of hepatic A4-steroid hydrogenase activity was carried out using corticosterone as substrate since this is the principle steroid secreted by the rat adrenal (Bush, 1953).…”
THE reduction of Ring A of adrenal steroids by the A4-steroid hydrogenases of liver has been shown in man (Peterson, Wyngaarden, Guerra, Brodie and Bunim, 1958) and in the rat (Glenister and Yates, 1961) to be the rate-controlling step in the inactivation of corticosteroids. Urquhart, Yates and Herbst (1959) (Urquhart et al., 1959). These authors therefore concluded that it is the level of hepatic A4-steroid hydrogenase activity which determines adrenal size rather than the reverse.Since adrenal hypertrophy is a common feature of tumour-bearing animals (Begg, 1958) the present study was undertaken to determine whether the presence of a growing tumour affected the rate of hepatic reduction of Ring A of adrenal steroids.
EXPERIMENTAL METHODS
AnimalsVirgin female albino rats were fasted overnight and those weighing 140-160 g. were selected and housed in individual cages.
Treatment of animalsThe animals were anaesthetised with ether and the experimental group injected intramuscularly into the right thigh with 1 ml. portions of a suspension of Walker 256 carcinoma, prepared as described by Talalay, Takano and Huggins (1952). The control group received an intramuscular injection of 1 mrnl. 0.9 per cent saline.Throughout the experimental period both groups of rats were fed a diet adequate in calories and protein content as described previously (Clark and Goodlad, 1960).The rats were killed at various time intervals after injection by stunning and exsanguination. The liver, adrenal glands and tumour were rapidly removed .znd weighed.
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