Species boundaries of Gulf of Mexico vestimentiferans (Polychaeta, Siboglinidae) inferred from mitochondrial genes

Miglietta, Maria Pia; Hourdez, Stéphane; Cowart, Dominique A.; Schaeffer, Stephen W.; Fisher, Charles R.

doi:10.1016/j.dsr2.2010.05.007

Cited by 38 publications

(47 citation statements)

References 46 publications

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“…muricola, and B. childressi, as well as Bathynerita naticoidea and K. cf. meroglypta also dominate both the El Pilar seeps and several Gulf of Mexico seeps of similar depths (Sibuet and Olu, 1998;Cordes et al, 2007b;Miglietta et al, 2010;Olu et al, 2010).…”

Section: Biogeographical Comparisons With In the Regionmentioning

confidence: 99%

Characterization of Methane-Seep Communities in a Deep-Sea Area Designated for Oil and Natural Gas Exploitation Off Trinidad and Tobago

et al. 2017

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Exploration of the deep ocean (>200 m) is taking on added importance as human development encroaches. Despite increasing oil and natural gas exploration and exploitation, the deep ocean of Trinidad and Tobago is almost entirely unknown. The only scientific team to image the deep seafloor within the Trinidad and Tobago Exclusive Economic Zone was from IFREMER in the 1980s. That exploration led to the discovery of the El Pilar methane seeps and associated chemosynthetic communities on the accretionary prism to the east of Trinidad and Tobago. In 2014, the E/V Nautilus, in collaboration with local scientists, visited two previously sampled as well as two unexplored areas of the El Pilar site between 998 and 1,629 m depth using remotely operated vehicles. Eighty-three megafaunal morphospecies from extensive chemosynthetic communities surrounding active methane seepage were observed at four sites. These communities were dominated by megafaunal invertebrates including mussels (Bathymodiolus childressi), shrimp (Alvinocaris cf. muricola), Lamellibrachia sp. 2 tubeworms, and Pachycara caribbaeum. Adjacent to areas of active seepage was an ecotone of suspension feeders including Haplosclerida sponges, stylasterids and Neovermilia serpulids on authigenic carbonates. Beyond this were large Bathymodiolus shell middens. Finally there was either a zone of sparse octocorals and other nonchemosynthetic species likely benefiting from the carbonate substratum and enriched production within the seep habitat, or sedimented inactive areas. This paper highlights these ecologically significant areas and increases the knowledge of the biodiversity of the Trinidad and Tobago deep ocean. Because methane seepage and chemosynthetic communities are related to the presence of extractable oil and gas resources, development of best practices for the conservation of biodiversity in Trinidad and Tobago waters within the context of energy extraction is critical. Potential impacts on benthic communities during oil and gas activities will likely be long lasting and include physical disturbance during drilling among others. Recommendations for the stewardship of Amon et al.Seep Communities Off Trinidad and Tobago these widespread habitats include: (1) seeking international cooperation; (2) holding wider stakeholder discussions; (3) adopting stringent environmental regulations; and (4) increasing deep-sea research to gather crucial baseline data in order to conduct appropriate marine spatial planning with the creation of marine protected areas.

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Section: Biogeographical Comparisons With In the Regionmentioning

confidence: 99%

Characterization of Methane-Seep Communities in a Deep-Sea Area Designated for Oil and Natural Gas Exploitation Off Trinidad and Tobago

et al. 2017

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“…In comparison, Marker X18 was characterized by two species of tubeworms identified via barcoding as Lamellibrachia sp. 2 (100% match with sequences from the Gulf of Mexico; Miglietta et al, 2010) and Escarpia sp. (100% match with sequences from the Escarpia laminata/Escarpia southwardae/Escarpia spicata group in Miglietta et al, 2010).…”

Section: Von Damm Vent Fieldmentioning

confidence: 92%

“…2 (100% match with sequences from the Gulf of Mexico; Miglietta et al, 2010) and Escarpia sp. (100% match with sequences from the Escarpia laminata/Escarpia southwardae/Escarpia spicata group in Miglietta et al, 2010). R. hybisae and I. bathycodon were also present at Marker X18 (Fig.…”

Section: Von Damm Vent Fieldmentioning

confidence: 92%

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Characterization of vent fauna at the Mid-Cayman Spreading Center

Plouviez

Jacobson

et al. 2015

Deep Sea Research Part I: Oceanographic Research Papers

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“…PCR reactions and gel electrophoresis were conducted as described in Miglietta et al (2010). Purified PCR products were submitted to The Penn State Genomics Core Facility (University Park, PA) and run on a 3730XL DNA sequencer.…”

Section: Methodsmentioning

confidence: 99%

Investigation of population structure in Gulf of MexicoSeepiophila jonesi(Polychaeta, Siboglinidae) using cross-amplified microsatellite loci

Huang

Schaeffer

Fisher

et al. 2016

PeerJ

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BackgroundVestimentiferan tubeworms are some of the most recognizable fauna found at deep-sea cold seeps, isolated environments where hydrocarbon rich fluids fuel biological communities. Several studies have investigated tubeworm population structure; however, much is still unknown about larval dispersal patterns at Gulf of Mexico (GoM) seeps. As such, researchers have applied microsatellite markers as a measure for documenting the transport of vestimentiferan individuals. In the present study, we investigate the utility of microsatellites to be cross-amplified within the escarpiid clade of seep vestimentiferans, by determining if loci originally developed for Escarpia spp. could be amplified in the GoM seep tubeworm, Seepiophila jonesi. Additionally, we determine if cross-amplified loci can reliably uncover the same signatures of high gene flow seen in a previous investigation of S. jonesi.MethodsSeventy-seven S. jonesi individuals were collected from eight seep sites across the upper Louisiana slope (<1,000 m) in the GoM. Forty-eight microsatellite loci that were originally developed for Escarpia laminata (18 loci) and Escarpia southwardae (30 loci) were tested to determine if they were homologous and polymorphic in S. jonesi. Loci found to be both polymorphic and of high quality were used to test for significant population structuring in S. jonesi.ResultsMicrosatellite pre-screening identified 13 (27%) of the Escarpia loci were homologous and polymorphic in S. jonesi, revealing that microsatellites can be amplified within the escarpiid clade of vestimentiferans. Our findings uncovered low levels of heterozygosity and a lack of genetic differentiation amongst S. jonesi from various sites and regions, in line with previous investigations that employed species-specific polymorphic loci on S. jonesi individuals retrieved from both the same and different seep sites. The lack of genetic structure identified from these populations supports the presence of significant gene flow via larval dispersal in mixed oceanic currents.DiscussionThe ability to develop “universal” microsatellites reduces the costs associated with these analyses and allows researchers to track and investigate a wider array of taxa, which is particularly useful for organisms living at inaccessible locations such as the deep sea. Our study highlights that non-species specific microsatellites can be amplified across large evolutionary distances and still yield similar findings as species-specific loci. Further, these results show that S. jonesi collected from various localities in the GoM represents a single panmictic population, suggesting that dispersal of lecithotrophic larvae by deep sea currents is sufficient to homogenize populations. These data are consistent with the high levels of gene flow seen in Escarpia spp., which advocates that differences in microhabitats of seep localities lead to variation in biogeography of separate species.

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Species boundaries of Gulf of Mexico vestimentiferans (Polychaeta, Siboglinidae) inferred from mitochondrial genes

Cited by 38 publications

References 46 publications

Characterization of Methane-Seep Communities in a Deep-Sea Area Designated for Oil and Natural Gas Exploitation Off Trinidad and Tobago

Characterization of Methane-Seep Communities in a Deep-Sea Area Designated for Oil and Natural Gas Exploitation Off Trinidad and Tobago

Characterization of vent fauna at the Mid-Cayman Spreading Center

Investigation of population structure in Gulf of MexicoSeepiophila jonesi(Polychaeta, Siboglinidae) using cross-amplified microsatellite loci

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