“…Immediately after collection, blood sample was transferred into two tubes, a heparinized tube and non-heparinized one. Heparinized whole blood samples were used for the determination of hemoglobin concentration according to Pilaski (1972) and haematocrit value, as described by Hunsaker (1969). The separated sera from non-heparinized blood samples were used for the colorimetric estimation of levels of glucose (Trinder, 1969), total protein (Henry et al 1974), albumin (Doumas et al 1977), cholesterol according to Watson (1960) and total lipids according to Zollner and Kirsch (1962), using commercial kits.…”