Speciation, quantification and stability of selenomethionine, S-(methylseleno)cysteine and selenomethionine Se-oxide in yeast-based nutritional supplements

Amoako, Prince Osei; Kahakachchi, Chethaka; Dodova, Elena N.; Uden, Peter C.; Tyson, Julian F.

doi:10.1039/b703381h

Cited by 29 publications

(22 citation statements)

References 42 publications

(62 reference statements)

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“…We have previously reported a similar speciation pattern on subjecting selenized yeast samples to brief heating at elevated temperature [52]. In the present study, selenium species in some commercially available selenium supplements are determined.…”

Section: Resultssupporting

confidence: 53%

“…In 2004, we reported [51] the presence of a sulfur-selenium amino acid, S-(methylseleno)cysteine, in archived selenized yeast samples from the Clark study [42]. Its presence was subsequently confirmed by GC-AED [52] and 77 Se NMR [59]. Gammelgaard et al investigated the oxidative degradation of selenomethionine and followed the formation of selenomethionine Se-oxide [66].…”

Section: Resultsmentioning

confidence: 99%

“…The operating conditions with a cross flow nebulizer and Scott spray chamber were as follows: RF power 1100 W, plasma Ar flow rate 15 L min −1 , nebulizer Ar flow rate 0.98 L min −1 , auxiliary gas flow rate 1.20 L min −1 , methane collision cell gas 0.6 mL min −1 , isotopes monitored 78 Se, 80 Se, 72 Ge, dwell time 80 ms [52]. The HPLC system which utilized a 5-m Symmetry Shield TM RP-C 8 column (3.9 mm × 15 cm) (Waters Corporation, Milford, MA) and an ion-pairing mobile phase of 0.1% heptafluorobutanoic acid (HFBA), 1% methanol and 99% water (v/v) was described previously [40,51].…”

Section: Instrumentationmentioning

confidence: 99%

“…Enzymatic digestion using Protease XIV was as reported elsewhere [52,53] and is summarized below. About 0.2 g of powdered sample was added to 0.02 g of Protease XIV in 15-mL centrifuge tube with 5 mL of water and was shaken for 24 h at room temperature, centrifuged at 3,000 g for 20 min and filtered through a 0.45-m polypropylene filter and a 10,000 Da molecular weight cut-off filter.…”

Section: Enzymatic Extraction For Hplc and Gcmentioning

confidence: 99%

“…Most dramatically the SELECT trial that utilized l-selenomethionine as the active supplement, was terminated in 2008 following a conclusion that there had been no demonstrable beneficial effect on prevention of prostate cancer and there was a possible indication of increased incidence of diabetes [50]. Since selenized yeast, as employed by Clark et al and in most supplements, was not tested in the SELECT study, it is conceivable that this difference proved important and that other selenium species observed in the 'Clark' yeast were germane to those results [51,52]. Alternatively Rayman et al have argued that the null results of SELECT are explainable by the fact that the candidate subjects already had optimal selenoprotein concentration/activity (notably selenoprotein P) and the results of SELECT tell nothing regarding effects of selenium on advanced cancer or on men of low Se status [53][54][55].…”

Section: Introductionmentioning

confidence: 99%

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Speciation of selenium dietary supplements; formation of S-(methylseleno)cysteine and other selenium compounds

Amoako

Uden

Tyson

2009

Analytica Chimica Acta

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a b s t r a c tSpeciation of selenium is of interest because it is both essential and toxic to humans, depending on the species and the amount ingested. Following indications that selenium supplementation could reduce the incidence of some cancers, selenium-enriched yeast and other materials have been commercialized as supplements. Most dramatically however, the SELECT trial that utilized l-selenomethionine as the active supplement was terminated in 2008 and there is much debate regarding both the planning and the results of efficacy studies. Further, since dietary supplements are not regulated as pharmaceuticals, there are concerns about the quality, storage conditions, stability and selenium content in selenium supplements. Enzymatic hydrolysis enabled selenium speciation profiles to be obtained by high performance liquid chromatography with inductively coupled plasma mass spectrometry (HPLC-ICP-MS) and following derivatization gas chromatography with atomic emission detection (GC-AED). Coated fiber solid phase microextraction (SPME) was used to extract volatile selenium species for determination by GC-AED and GC-MS. Similar speciation patterns were observed between yeast-based supplements subject to extended storage and those heated briefly at elevated temperatures. All the yeast-based supplements and one yeast-free supplement formed S-(methylseleno)cysteine on heating. Evidence was obtained in support of the hypotheses that S-(methylseleno)cysteine is formed from a reaction between dimethyldiselenide and cysteine or cystine.

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Section: Resultssupporting

confidence: 53%

Section: Resultsmentioning

confidence: 99%

Section: Instrumentationmentioning

confidence: 99%

Section: Enzymatic Extraction For Hplc and Gcmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Speciation of selenium dietary supplements; formation of S-(methylseleno)cysteine and other selenium compounds

Amoako

Uden

Tyson

2009

Analytica Chimica Acta

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Selenized lactic acid bacteria microencapsulated by spray drying: A promising strategy for beef cattle feed supplementation

Schwingel Henn,

Cima,

Bettanin

et al. 2024

Biotech and App Biochem

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This study aimed to assess the technical feasibility of incorporating selenized Lactobacillus spp. microencapsulated via spray drying into cattle feed. Gum Arabic and maltodextrin were used as encapsulating agents. The encapsulation process was carried out with a drying air flow rate of 1.75 m3/min, inlet air temperature of 90°C, and outlet air temperature of 75°C. The viability of the encapsulated microorganisms and the technological characteristics of the obtained microparticles were evaluated. Microorganisms were incorporated into beef cattle feed to supplement their diet with up to 0.3 mg of Se per kilogram of feed. The encapsulated particles, consisting of a 50/50 ratio of gum Arabic/maltodextrin at a 1:20 proportion of selenized biomass to encapsulant mixture, exhibited superior technical viability for application in beef cattle feed. Supplemented feeds displayed suitable moisture, water activity, and hygroscopicity values, ensuring the preservation of viable microorganisms for up to 5 months of storage, with an approximate count of 4.5 log CFU/g. Therefore, supplementing beef cattle feed with selenized and microencapsulated lactic acid bacteria represents a viable technological alternative, contributing to increased animal protein productivity through proper nutrition.

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Separation and determination of seleno amino acids using gas chromatography hyphenated with inductively coupled plasma mass spectrometry after hollow fiber liquid phase microextraction

Duan

2008

J. Mass Spectrom.

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A new derivatization-extraction method for preconcentration of seleno amino acids using hollow fiber liquid phase microextraction (HF-LPME) was developed for the separation and determination of seleno amino acids in biological samples by gas chromatography-inductively coupled plasma mass spectrometry (GC-ICP-MS). Derivatization was performed with ethyl chloroformate (ECF) to improve the volatility of seleno amino acids. Parameters influencing microextraction, including extraction solvent, pH of sample solution, extraction time, stirring speed, and inorganic salt concentration have been investigated. Under the optimal conditions, the limits of detection (LODs) obtained for Se-methyl-selenocysteine (SeMeCys), selenomethionine (SeMet), and selenoethionine (SeEth) were 23, 15, and 11 ng Se l(-1), respectively. The relative standard deviations (RSDs) were 14.6%, 16.4%, and 19.4% for SeMeCys, SeMet, and SeEth (c = 1.0 ng ml(-1), n = 7), respectively, and the RSDs for SeMeCys, SeMet could be improved obviously if SeEth was utilized as the internal standard. The proposed method was applied for the determination of seleno amino acids in extracts of garlic, cabbage, and mushroom samples, and the recoveries for the spiked samples were in the range of 96.8-108% and 93.4-115% with and without the use of SeEth as internal standard. The developed method was also applied to the analysis of SeMet in a certified reference material of SELM-1 yeast and the determined value is in good agreement with the certified value.

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Speciation, quantification and stability of selenomethionine, S-(methylseleno)cysteine and selenomethionine Se-oxide in yeast-based nutritional supplements

Cited by 29 publications

References 42 publications

Speciation of selenium dietary supplements; formation of S-(methylseleno)cysteine and other selenium compounds

Speciation of selenium dietary supplements; formation of S-(methylseleno)cysteine and other selenium compounds

Selenized lactic acid bacteria microencapsulated by spray drying: A promising strategy for beef cattle feed supplementation

Separation and determination of seleno amino acids using gas chromatography hyphenated with inductively coupled plasma mass spectrometry after hollow fiber liquid phase microextraction

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