Speciation of Selenium in Enriched Garlic Sprouts by High-Performance Liquid Chromatography Coupled with Inductively Coupled Plasma-Mass Spectrometry

Zhong, Nanshan; Zhong, Li; Liu, Hao; Cheng-zhang, Luan; Li, Xinrong

doi:10.1080/00032719.2014.930875

Cited by 18 publications

(3 citation statements)

References 19 publications

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“…Plants cultured with 16 µM Se presented a decrease in the Chltotal content (Table 1). Therefore, these plants could undergo a photodestructive effect caused by the greater degradation rates and reduction in the biosynthesis of these pigments (Tausz et al 2001), possibly related to the toxic effect of the high concentrations of Se (Zhong et al 2015).…”

Section: Discussionmentioning

confidence: 99%

Selenium improves the transport dynamics and energy conservation of the photosynthetic apparatus of in vitro grown Billbergia zebrina (Bromeliaceae)

Souza¹,

Martins²,

Gontijo³

et al. 2019

Photosynt.

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Despite having beneficial effects, selenium is not an essential element for plants and its action mechanisms are still unclear. In this context, we evaluated effects of selenium, considering possible modulations of the photosynthetic apparatus of Billbergia zebrina plants during in vitro culture. Lateral shoots of B. zebrina were grown in a medium with different concentrations of selenium (0, 2, 4, and 16 μM). After 75 d, concentrations of photosynthetic pigments, growth traits, and chlorophyll a fluorescence were evaluated. In low concentrations, selenium increased the potential energy conservation capacity of the photosynthetic apparatus, maintained the PSII energy transport reaction stability, and improved the electron transport dynamics between the intersystem and PSI. In addition, B. zebrina showed physiological disturbances at Se concentrations equal or greater than 16 μM, presenting reduced growth and photosynthetic pigment contents and impaired photosynthetic apparatus. Abbreviations: ABS/RC -absorption flux per RC; Chl -chlorophyll; Chltotal -total chlorophyll; DI0/RC -dissipated energy flux per RC; ET0/RC -electron transport flux per RC; F0 -minimal fluorescence yield of the dark-adapted state; FI -fluorescence intensity at 30 ms; FJ -fluorescence intensity at 2 ms; FK -fluorescence intensity at 0.3 ms; Fm -maximal fluorescence yield of the dark-adapted state; FM -fresh mass; FP -fluorescence intensity at 300 ms; OCE -oxygen-evolving complex; Pheo -pheophytin; PI(ABS) -performance index based on absorption; PI(Total) -overall performance index, which measures the performance up until the final electron acceptors of PSI; RC -reaction center; RC/ABS -total number of active reaction center per absorption; RE0/RC -reduction of end acceptors at PSI electron acceptor side per RC; ROS -reactive oxygen species; TR0/RC -trapping flux per RC; VI -relative variable fluorescence at 30 ms (point I); VJ -relative variable fluorescence at 2 ms (point J); δR0 -efficiency/probability with which an electron from the intersystem electron carriers moves to reduce end electron acceptors at the PSI acceptor side; φD0 -quantum yield of energy dissipation; φE0 -quantum yield of electron transport; φP0 -maximum quantum yield of primary photochemistry; φR0 -quantum yield of reduction of end electron acceptors at the PSI acceptor side; ρR0 -efficiency with which a trapped exciton can move an electron into the electron transport chain from QA − to the PSI end electron acceptors; ψE0 -probability that a trapped exciton moves an electron into the electron transport chain beyond QA − . Acknowledgments: The authors would like to acknowledge the scholarship awarded by the CNPq (Brazilian National Council for Scientific and Technological Development) and the FAPES (Espírito Santo State Research Foundation).

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Section: Discussionmentioning

confidence: 99%

Selenium improves the transport dynamics and energy conservation of the photosynthetic apparatus of in vitro grown Billbergia zebrina (Bromeliaceae)

Souza¹,

Martins²,

Gontijo³

et al. 2019

Photosynt.

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“…The optimum separation conditions were an eluent containing 1-butanesulfonic acid (8 mM) and triuoroacetic acid (4 mM) at pH 4.5 using a ow rate of 1.2 mL min À1 and a RP C 18 column (250 Â 4.6 mm). The production of Se-metabolites by garlic (Allium Sativum L) cultivated in a selenite-enriched medium was explored by HPLC-ICP-MS. 160 Optimal separation conditions were achieved using a RP separation C 18 column (250 Â 4.6 mm, 5 mm) and a buffer of 20 mmol L À1 ammonium acetate containing 5% (v/v) methanol as the mobile phase. The group of Lobinski 156 continues to investigate Se metabolism in plants by applying a multistep approach combining three chromatographic clean-up steps, ion exchange, reversed phase and ion pairing, and ES-TOF-MS, ES-Orbitrap-MS and ICP-MS detection.…”

Section: Studies On the Conversions And Transformations Of Se In Planmentioning

confidence: 99%

“…The amount of total Se ranged from 45.9 to 216 mg kg À1 and no iSe was detected, with SeMet as the pre-eminent organoselenium species in the extracts. The production of Se-metabolites by garlic (Allium Sativum L) cultivated in a selenite-enriched medium was explored by HPLC-ICP-MS. 160 Optimal separation conditions were achieved using a RP separation C 18 column (250 Â 4.6 mm, 5 mm) and a buffer of 20 mmol L À1 ammonium acetate containing 5% (v/v) methanol as the mobile phase. Four extractant solutions: 0.1 mol L À1 HCl, 0.1 mol L À1 NaOH, 20 mmol L À1 ammonium acetate-5% methanol and protease XIV were compared and the most suitable results were obtained using 0.1 mol L À1 HCl.…”

Section: Seleniummentioning

confidence: 99%