Aim:The aim of the present study is to confirm the species formed under the used experimental conditions and to validate the models by statistical treatment of the data. As the results obtained in this study have specific applications in extraction metallurgy, nuclear energy industry, and analytical methods, medical, environmental, and industrial research. These applications all require stability constant values of high reliability and sources of critically evaluated published constants are referenced. Stability constant is useful physical entity, which explains the importance and function of various complexes in biological systems. Background: Despite the availability of information about the effect of organic-water media, anionic-surfactants media on the stability of binary complexes, this data related to 5-Hydroxysalicylic acid with essential metals Ca (II), Mg (II), Zn (II) in cationic-surfactant medium is carried out in this study. Materials and Methods: Aqueous solutions of Ca, Mg Zn and Cetyltrimethylammonium Bromide were prepared using triple-distilled water. The alkali metric titrations were carried out in a medium containing varying compositions of Cetyltrimethylammonium Bromide (CTAB)-water mixtures (0.0-2.5% w/v) used by maintaining an ionic strength of 0.16 mol dm -3 with sodium hydroxide at 303.0+0.1 K. An ELICO (Model L1-120) pH-meter (readability 0.01 pH units) pH meter was used to carry out the pH values. A well-defined computer algorithm known as MINIQUAD75 was used to perform an analysis on the complex species models. On a few different complex species, exhaustive modelling work has been done. Results: The formed metal-ligand complexes in between Ca(II), Mg(II), Zn(II) and Cetyltrimethylammonium bromide are ML 2 H 3 , ML 2 H 4 , and ML 3 H 4 . These complexes have been identified to be the predominant complex species that have formed. On the basis of the statistical parameters, the chemical modelling approach that provides the best fit has been adopted. The complex species were verified through the statistical study of the data. Based on electrostatics, the change in stability of complex species as a function of surfactant composition has been shown, the distribution of chemical species with respect to pH and compositional diversity is also shown explores how possible complicated equilibria. The data acquisition of acid-base equilibria and determination of stability constants were performed using MINIQUAD75 algorithm and the distribution patterns of the complexes with varying pH and compositions of surfactants were presented from the plots of SIM run data. Conclusion: A study of the chemical speciation of ternary complexes of Ca(II), Mg(II), and Zn(II) with 5-HSA in micellar medium reveals the compartmentalization of metabolic reactions. The study gives an insight into the metal availability /metal transport in bio fluids. The binary complexes make the ''metal available'' in biological systems due to their decreased stability. It is observed that the stability constants are more affected by con...