Speciation of iron coordinated by phytosiderophores by use of HPLC with pulsed amperometric detection and AAS

Weber, G.; Neumann, G.; Römheld, Volker

doi:10.1007/s00216-002-1302-2

Cited by 23 publications

(13 citation statements)

References 24 publications

(37 reference statements)

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“…However, the biggest problem in nearly all published analytical methods for trace metal speciation is not the detection, but the unknown stability of metal species with respect to the (liquid chromatographic) separation. Such problems were reported for Ni species [23], Fe species [22], and even for PS species that are thermodynamically very stable, partial dissociation may happen on chromatographic columns [26,27]. One solution to this dilemma could be the use of direct methods for metal speciation, i.e., methods that are not based on a preceding separation.…”

Section: Introductionmentioning

confidence: 93%

“…Both methods are applicable for quantitative analyses of PS in the low micromolar concentration range, but are not well suited for the detection of intact metal species. In the last few years, some hyphenated analytical methods for detection and quantitative determination of metal-PS species have been proposed, based on HPLC with atomic absorption spectrometric detection [22], HPLC with inductively coupled plasma mass spectrometric detection (ICP/MS) [23][24][25][26], or HPLC-ESI/MS [24,25,27]. Clearly, the ICP/MS methods offer very good sensitivity and element specificity, but lack unequivocal identification of unknown species.…”

Section: Introductionmentioning

confidence: 99%

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CE of phytosiderophores and related metal species in plants

et al. 2007

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Phytosiderophores (PS) and the closely related substance nicotianamine (NA) are key substances in metal uptake into graminaceous plants. Here, the CE separation of these substances and related metal species is demonstrated. In particular, the three PS 2'-deoxymugineic acid (DMA), mugineic acid (MA), and 3-epi-hydroxymugineic acid (epi-HMA), and NA, are separated using MES/Tris buffer at pH 7.3. Moreover, three Fe(III) species of the different PS are separated without any stability problems, which are often present in chromatographic analyses. Also divalent metal species of Cu, Ni, and Zn with the ligands DMA and NA are separated with the same method. By using a special, zwitterionic CE capillary, even the separation of two isomeric Fe(III) chelates with the ligand ethylenediamine-N,N'-bis(o-hydroxyphenyl)acetic acid (EDDHA) is possible (i.e., meso-Fe(III)-EDDHA and rac-Fe(III)-EDDHA), and for fast separations of NA and respective divalent and trivalent metal species, a polymer CE microchip with suppressed EOF is described. The proposed CE method is applicable to real plant samples, and enables to detect changes of metal species (Cu-DMA, Ni-NA), which are directly correlated to biological processes.

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Section: Introductionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

CE of phytosiderophores and related metal species in plants

et al. 2007

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“…33,34,[45][46][47][48][49][50][51][52][53][54][55][56][57] Nestes sistemas, a principal função da detecção pulsada foi promover a limpeza eletroquímica constante e ativação da superfície do eletrodo de trabalho. A amplitude, polaridade e tempos de aplicação dos pulsos de potenciais, de detecção, limpeza e ativação podem ser variados, no sentido de se garantir a maior sensibilidade e estabilidade do sinal eletroquímico para uma diversidade de analitos.…”

Section: Limpeza E Ativação Da Superfície Do Eletrodo Através Da Mpaunclassified

Análise por injeção em fluxo com detecção amperométrica de múltiplos pulsos: potencialidades e aplicações

Santos¹,

Gimenes²,

Richter³

et al. 2011

Quím. Nova

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Recebido em 29/11/10; aceito em 15/3/11; publicado na web em 5/5/11 FLOW INJECTION ANALYSIS WITH MULTIPLE PULSE AMPEROMETRIC DETECTION: POTENTIALITIES AND APPLICATIONS. The potentialities and applications of the Multiple Pulse Amperometric detection (MPA) coupled with Flow Injection Analysis (FIA) are evaluated. Important aspects as cleaning and activation of electrode surface, indirect and simultaneous analysis of electroactive compounds and the use of the internal standard method for quantifications utilizing FIA-MPA are presented. The main parameters concerning the detection of electroactive analytes by multiple pulse amperometric detection in flowing solutions were also discussed. In addition, aspects such as flow rate, sample volume, application time of the potential pulses and instrumentation necessary for implementing of the method were also addressed.Keywords: electrochemical detection; multiple pulse amperometry; flow injection analysis. INTRODUÇÃOO desenvolvimento de métodos analíticos que combinam menor tempo de análise, maior sensibilidade e seletividade, simplicidade de manuseio, baixo custo e potencialidade adequada para automação constitui-se num campo de pesquisa amplo e em constante expansão na área de Química Analítica. Nesse contexto, a análise por injeção em fluxo, FIA -Flow Injection Analysis, tem sido explorada com sucesso na análise de grande diversidade de substâncias, proporcionando várias vantagens, tais como, uso de instrumentação versátil, baixo custo dos componentes do sistema, elevada frequência de amostragem, consumo reduzido de reagentes e amostras e, consequentemente, redução na geração de resíduos. 1-7 Além disto, o sistema FIA pode ser automatizado, minimizando os riscos de intoxicação do analista devido a uma menor exposição aos reagentes e amostras em comparação aos procedimentos manuais. Os primeiros trabalhos empregando FIA foram propostos por Rüzicka e Hansen, em 1975, como um novo conceito de análises químicas. 8,9 No primeiro artigo, os autores descrevem o sistema FIA acoplado a detectores de absorção molecular. 8 Três anos depois, os mesmos autores fazem uma revisão sobre os dez trabalhos que haviam desenvolvido anteriormente, abordando a teoria e as tendências da técnica. 9 Posteriormente, uma diversidade de técnicas foram exploradas, cabendo destacar a absorção atômica, 10,11 fluorescência, 12,13 quimiluminescência, 14,15 potenciometria, 16,17 voltametria, 18,19 amperometria, 20,21 entre outros.A detecção eletroquímica pode oferecer vantagens frente a outros sistemas de detecção, como baixo custo de instrumentação, simplicidade de aplicação e minimização de etapas de preparação de amostras. 3,[22][23][24][25] Adicionalmente, diversas espécies a serem analisadas podem ser pré-concentradas sobre eletrodos, tanto por processos de adsorção como por eletrodeposição. Os detectores eletroquímicos mais utilizados em sistemas em fluxo são os amperométricos, que foram adotados em grande parte dos trabalhos descritos na literatura. 3 Nesta técnica, o eletrodo de trabalho (ou se...

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“…For analysis of chemical speciation, different methods have been employed by different researchers such as voltammetry (Bard and Faulkner, 1980;Bond, 1980), liquid -liquid extraction (Meera et al, 2001;Francis et al, 2001), ion exchange and adsorption columnsyresins (Morel et al, 2008;Sweileh et al, 1987), gas chromatography (Dietz et al, 2000;Rodil et al, 2002), liquid chromatography (Ibrahim et al, 1984;Weber et al, 2002), capillary electrophoresis (CE) (Rocha et al, 2000;Yin et al, 2002) as well as inductively coupled plasma mass spectrometry (ICP-MS) (Medel et al, 2003;Butcher, 2007). ICP-MS could be useful tool for sensitive speciation analyses of many environmentally important elements.…”

Section: Introductionmentioning

confidence: 99%

Speciation of heavy metals in the surface waters of a former tin mining catchment

Ashraf

Maah

Yusoff

et al. 2012

Chemical Speciation & Bioavailability

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This study was conducted to investigate the chemical speciation of dissolved and particulate elements (Pb, Zn, Cu, Cr, As and Sn) in the mining wastewater. Speciation patterns of dissolved elements were estimated by adsorptive stripping voltammeter while particulate elements were analysed using a newly developed sequential extraction leaching procedure. The procedure has been operationally defined among five host fractions, namely exchangeable, carbonate, reducible, organic bound and residual. A total of six elements (Pb, Zn, Cu, Cr, As and Sn) were analysed in 30 samples at 10 locations (P1 -P10) representing three subsequent samples at the same location to obtain the average value from ex-tin mining catchment. The results showed that the heavy metal pollution in P4 and P8 was more severe than in other sampling sites, especially Sn and Pb pollution. In the water samples at P4 and P8, both the total contents and the most dangerous non-residual fractions of Sn and Pb were extremely high. More than 90% of the total concentrations of As and Cr existed in the residual fraction. Cu and Zn mainly (more than 60%) occurred in the residual fraction. However, Pb and Sn were predominantly present in the non-residual fractions of the surface water. For all the six dissolved elements, the less labile species formed the predominant fraction in their speciation patterns. We conclude that the speciation patterns of particulate elements show that most of the Pb, Zn, Cu, Cr, As and Sn were found in the reducible fraction whereas Pb and Sn were mainly associated with the organic fraction.

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Speciation of iron coordinated by phytosiderophores by use of HPLC with pulsed amperometric detection and AAS

Cited by 23 publications

References 24 publications

CE of phytosiderophores and related metal species in plants

CE of phytosiderophores and related metal species in plants

Análise por injeção em fluxo com detecção amperométrica de múltiplos pulsos: potencialidades e aplicações

Speciation of heavy metals in the surface waters of a former tin mining catchment

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