Speciation of fission products in raw cow milk by high-performance size exclusion radiochromatography

Abstract

Speciation of fission products (9~ 99Tc, 137Cs and 152Eu) in raw cow milk was studied using the high-performance size exclusion radiochromatography (HP-SERC) by Biosep-SEC-S | column (Phenomenex, USA). The separation fraction were identified by a UV detector (280 nm) and a scintillation radiodetector. The mobile phase was Jenness-Koops buffer that simulated milk serum. Radiocesium was present only in non-protein fractions. The non-protein fractions complexed 17% of technetium and 57% of strontium. About 32% of strontium and 24% of europium were bound on the high molecular weight proteins (micellar casein). Low molecular weight proteins bound about 11% of strontium and 76% of europium. Non-size effects play a dominant role at HPSERC speciation of technetium, 83% of which follow the lightest fractions of milk.

Speciation analysis for iodine in milk by size-exclusion chromatography with inductively coupled plasma mass spectrometric detection (SEC-ICP MS)

“…Studies of speciation of iodine in milk and body ¯uids have been practically non-existent because of the lack of a suitable, species-selective methodology, as demonstrated by the recent review papers. 17,18 In terms of potential methodology available for speciation analysis for iodine in milk, approaches described for speciation of essential elements (Zn, Cu, Fe, Se), 15,16,19,20 toxic elements (Cd) 21 and radioactive elements ( 90 Sr, 99 Tc, 137 Cs and 152 Eu) 22 in this matrix should be considered. Size-exclusion chromatography has been the most widely used fractionation technique for trace element species present in milk, whereas ICP AES, 15,16 ICP MS, 16 scintillation radiodetection 22 and stripping voltammetry 21 were the most commonly used detection techniques.…”

“…17,18 In terms of potential methodology available for speciation analysis for iodine in milk, approaches described for speciation of essential elements (Zn, Cu, Fe, Se), 15,16,19,20 toxic elements (Cd) 21 and radioactive elements ( 90 Sr, 99 Tc, 137 Cs and 152 Eu) 22 in this matrix should be considered. Size-exclusion chromatography has been the most widely used fractionation technique for trace element species present in milk, whereas ICP AES, 15,16 ICP MS, 16 scintillation radiodetection 22 and stripping voltammetry 21 were the most commonly used detection techniques. Since milk is a slurry that cannot be introduced on to the column directly, milk whey was actually analysed.…”