Speciation of Chromium by High-Performance Thin-Layer Chromatography with Direct Determination by Laser Ablation Inductively Coupled Plasma Mass Spectrometry

Abstract

It is of considerable importance to be able to distinguish metallic species because their toxicity depends on their chemical form. Therefore, the analysis of environmental samples can be enhanced by the combination of high-performance thin-layer chromatography (HPTLC) with laser ablation inductively coupled plasma mass spectrometry (LA-ICPMS). In this study, Cr (3+) and Cr (6+) were separated on silica gel HPTLC plates using aqueous mobile phases. Separation was achieved in seconds with retardation factors ( R f ) of 0 and 1 for Cr (3+) and Cr (6+), respectively. LA was used to volatilize the chromium species directly from the chromatographic material prior to ICPMS detection. A linear calibration was obtained, and detection limits (3sigma) of 6 ng for Cr (6+) and 0.4 ng for Cr (3+) were achieved with precision ranging from 3 to 40% at the 95% confidence level. The silicon present in the stationary phase was used as an internal standard. This procedure allows for a rapid separation and quantification, requires only 0.5 muL of sample, and lower detection limits can be achieved through preconcentration.

Simultaneous analysis of Cr(III), Cr(VI), and chromium picolinate in foods using capillary electrophoresis‐inductively coupled plasma mass spectrometry

“…As we mentioned above, HPLC‐ICP‐MS and IC‐ICP‐MS techniques have been developed for the Cr(VI) and Cr(III) detection . Both HPLC‐ICP‐MS method and IC‐ICP‐MS method have high sensitivity.…”

Laser desorption-ion mobility spectrometry as a useful tool for imaging of thin layer chromatography surface

“…Mass spectrometry (MS) with different ionization sources is a powerful technique for detection and characterization of vast spectrum of chemical compounds. In TLC, the analyte is deposited on the plate, therefore, the analyte should be sampled from the TLC surface and gasified in order to be detected by MS. Coupling of TLC with MS has been carried out using different techniques and ionization sources such as laser desorption ionization (LDI) [6][7][8][9] secondary ion mass spectrometry (SIMS) [10] matrix-assisted laser desorption ionization (MALDI) [11][12][13][14][15] surface-assisted laser desorption ionization (SALDI) [16,17] electrospray ionization (EI) [18][19][20][21] electrospray laser desorption ionization (ELDI) [22,23] laser-induced acoustic desorption electrospray ionization (LIAD/ESI) [24] laser desorption/inductively coupled plasma (LD/ICP) [25,26] and so on. Also, different mass spectrometers including time of flight (TOF) ion trap and quadruple are used to detect and analyze the analytes sampled from the TLC surface.…”

Simultaneous Determination of Cr(III) and Cr(VI) by Differential Pulse Voltammetry Using Modified Screen‐Printed Carbon Electrodes in Array Mode

“…Many instrumental techniques have been used for the determination of the total content of chromium, such as ultraviolet-visible spectrophotometry [7], electrothermal atomic absorption spectrometry (ETAAS), flame atomic absorption spectrometry (FAAS) [3,7,8] inductively coupled plasma-mass spectrometry (ICP-MS) [9], inductively coupled plasma optical emission spectrometry (ICP-OES) [7] and electrochemical techniques including anodic stripping voltammetry (ASV) [10,11], differential pulse adsorptive stripping voltammetry (DPAdSV) [12] and catalytic adsorptive stripping voltammetry (CAdSV) [13][14][15]. These methods involve the determination of chromium after tedious separation steps.…”