Speciation of arsenic in tube‐well water samples collected from West Bengal, India, by high‐performance liquid chromatography–inductively coupled plasma mass spectrometry

Abstract

The objective of this study was to report on the arsenic species present in tube-well water samples collected from West Bengal, India, especially dimethylarsinic acid (DMA) and monomethylarsonic acid (MMA), whose existence has not been reported in the literature. The water samples were collected from Jalangi Gram Panchayet (Murshidabad district, West Bengal, India). The samples were speciated for arsenic 11 days after collection. The samples were collected in duplicate. One part was acidified with nitric acid (final concentration 0.1%), whereas the other part was left unacidified. A quick and highly sensitive high-performance liquid chromatography±inductively coupled plasma mass spectrometry (HPLC±ICPMS) technique was employed for the separation and detection of the arsenic species. Four arsenic species, namely arsenite [arsenic(III)], DMA, MMA and arsenate [arsenic(V)] were separated and analysed in less than 5 min. Total arsenic concentration was determined by flow injection (FI)-ICPMS. Most of the samples were found to contain low concentrations of DMA and MMA (<2.1 ppb) and high concentrations of inorganic arsenic (>300 ppb). The existence of DMA and MMA in both acidified and unacidified water samples and in similar concentrations suggests that their presence is natural and not due to acidification. The detection limit of the four arsenic species was 0.06±0.10 ppb. The method was validated by spike recovery and analysis of two water standard reference materials (SRMs). The percentage recoveries of added spikes of all four species were 97±112%. The total arsenic concentration obtained by FI-ICPMS and the sum of the four arsenic species obtained by HPLC±ICPMS for the two water SRMs agreed with the certified values. Moreover, the difference between the total arsenic and the sum of the four arsenic species for most of the water samples was less than 10%.

A fully validated method for the determination of arsenic species in rice and infant cereal products

“…Therefore, reagents in the blank did not induce interferences in the chromatograms. The presence of a high content of chloride (Cl -) in the matrices could lead to the misidentification of As with ICP-MS detection [45]. As commented above, the ion intensity at m/z 75 ( 75 As) was monitored and additionally, the ion intensities at …”

Arsenic Metabolites and Methylation Capacity Among Individuals Living in a Rural Area with Endemic Arseniasis in Inner Mongolia, China

“…Arsenic in drinking water is dominated by iAs as pentavalent (As V ) and trivalent arsenite (As III ) [14,15]. In humans, the primary iAs metabolic pathway is methylation, which involves reduction and oxidative methylation [16][17][18].…”

Distribution and Excretion of Arsenic Metabolites after Oral Administration of Seafood-Related Organoarsenicals in Rats

“…The major peak was eluted at a retention time corresponding to AB and/or iAs III ; high concentrations of chloride in the samples resulted in splitting and broadening of the iAs V and MMA V peaks ( Figure S2b) [41]. To confirm whether the first peak shown in Figure S2c was AB or iAs III and whether iAs V was contained in the basal diet or not, the samples were applied to an anion-exchange column ( Figure S2g).…”