2002
DOI: 10.1603/0022-2585-39.1.16
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Speciation and Distribution of the Members of theAnopheles punctulatus(Diptera: Culicidae) Group in Papua New Guinea

Abstract: Mosquito collections were made throughout the mainland of Papua New Guinea to identify the members of the Anopheles punctulatus group present and to determine their distribution. Identification was made using morphology, DNA hybridization, and polymerase chain reaction (PCR)-RFLP analysis. Nine members of the group were identified: An. farauti s.s. Laveran, An. farauti 2, An. koliensis Owen, and An. punctulatus Dönitz, were common and widespread; An. farauti 4 was restricted to the north of the central ranges … Show more

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Cited by 69 publications
(116 citation statements)
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“…hinesorum (n = 12), and An. farauti 4 (n = 16) showed considerable ITS2 sequence divergence between species (from 65.0% to 92.9% pairwise percent identity; includes previously published sequences 10 Given the variability of proboscis coloration and overlapping geographic distribution of morphologically similar Punctulatus group sibling species previously documented by Cooper and others 4 and Beebe and Cooper, 14 we anticipated that we would observe discordance between the two classification schemes (ITS2 LDR-FMA and morphological identification). At 10 of the 18 collection sites, 100% concordance was observed between DNA probe and morphologic classification of An.…”
Section: Discussionmentioning
confidence: 69%
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“…hinesorum (n = 12), and An. farauti 4 (n = 16) showed considerable ITS2 sequence divergence between species (from 65.0% to 92.9% pairwise percent identity; includes previously published sequences 10 Given the variability of proboscis coloration and overlapping geographic distribution of morphologically similar Punctulatus group sibling species previously documented by Cooper and others 4 and Beebe and Cooper, 14 we anticipated that we would observe discordance between the two classification schemes (ITS2 LDR-FMA and morphological identification). At 10 of the 18 collection sites, 100% concordance was observed between DNA probe and morphologic classification of An.…”
Section: Discussionmentioning
confidence: 69%
“…by morphological characteristics. 4,22 A set of 340 mosquitoes morphologically identified as members of the An. punctulatus group were then kept in coded vials containing silica gel until DNA extraction could be completed.…”
Section: Mosquitoesmentioning
confidence: 99%
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