Speciation analysis of Tc radiopharmaceuticals by HPLC-ICP-MS and HPLC-ESI-HRMS

Horstmann, Maximilian; Austrup, Melanie; Busch, Felix; Faust, Andreas; Sperling, Michael; Karst, Uwe; Clases, David

doi:10.1039/d3ja00257h

Cited by 4 publications

(1 citation statement)

References 32 publications

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“…The speciation of Tc (the most important radionuclide for SPECT in nuclear medicine) has been carried out by using HPLC-ICP-MS and HPLC-ES-HR-MS. 138 A C 18 column (accurate 2.6 mm, 150 mm) was employed to separate most of the Tc-species in several radiopharmaceutical products. Due to the lack of Tc standards, isobaric dilution analysis (IBDA) was applied to enable calibration.…”

Section: Elemental Speciation Analysismentioning

confidence: 99%

Atomic spectrometry update: review of advances in elemental speciation

Clough,

Harrington,

Hill

et al. 2024

J. Anal. At. Spectrom.

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Section: Elemental Speciation Analysismentioning

confidence: 99%

Atomic spectrometry update: review of advances in elemental speciation

Clough,

Harrington,

Hill

et al. 2024

J. Anal. At. Spectrom.

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Notizen aus der Chemie

Barra,

Blasco,

Delaittre

et al. 2023

Nachr Chem

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Quantification of [99Tc]TcO4- in urine by means of anion-exchange chromatography–aerosol desolvation nebulization–inductively coupled plasma–mass spectrometry

Horstmann,

Quarles,

Happel

et al. 2024

Anal Bioanal Chem

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To sensitively determine 99Tc, a new method for internal quantification of its most common and stable species, [99Tc]Tc$${\text{O}}_{4}^{-}$$ O 4 - , was developed. Anion-exchange chromatography (IC) was coupled to inductively coupled plasma–mass spectrometry (ICP-MS) and equipped with an aerosol desolvation system to provide enhanced detection power. Due to a lack of commercial Tc standards, an isotope dilution-like approach using a Ru spike and called isobaric dilution analysis (IBDA) was used for internal quantification of 99Tc. This approach required knowledge of the sensitivities of 99Ru and 99Tc in ICP-MS. The latter was determined using an in-house prepared standard manufactured from decayed medical 99mTc-generator eluates. This standard was cleaned and preconcentrated using extraction chromatography with TEVA resin and quantified via total reflection X-ray fluorescence (TXRF) analysis. IC coupled to ICP-MS enabled to separate, detect and quantify [99Tc]Tc$${\text{O}}_{4}^{-}$$ O 4 - as most stable Tc species in complex environments, which was demonstrated in a proof of concept. We quantified this species in untreated and undiluted raw urine collected from a patient, who previously underwent scintigraphy with a 99mTc-tracer, and determined a concentration of 19.6 ± 0.5 ng L−1. The developed method has a high utility to characterize a range of Tc-based radiopharmaceuticals, to determine concentrations, purity, and degradation products in complex samples without the need to assess activity parameters of 99(m)Tc. Graphical Abstract

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Speciation analysis of Tc radiopharmaceuticals by HPLC-ICP-MS and HPLC-ESI-HRMS

Abstract: 99mTc is commonly employed in radiotracers scintigraphy. New methods to inquire identity and to perform quantitative characterization are presented.

Cited by 4 publications

References 32 publications

Atomic spectrometry update: review of advances in elemental speciation

Atomic spectrometry update: review of advances in elemental speciation

Notizen aus der Chemie

Quantification of [99Tc]TcO4- in urine by means of anion-exchange chromatography–aerosol desolvation nebulization–inductively coupled plasma–mass spectrometry

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