Speciation Analysis of Selenium Metabolites in Yeast-Based Food Supplements by ICPMS—Assisted Hydrophilic Interaction HPLC—Hybrid Linear Ion Trap/Orbitrap MS<i><sup>n</sup></i>

Dernovics, Mihály; Łobiński, Ryszard

doi:10.1021/ac8002038

Cited by 67 publications

(58 citation statements)

References 48 publications

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“…This technique has been used in an in vitro absorption, distribution, metabolism, and excretion workflow of cassette incubation for as many as 32 compounds, followed by quantitative bioanalysis using full scan acquisition (64). Targeting of compounds can also now be achieved on the fly for a variety of analyte groups, including those containing metals (76) or those with distinct isotopic patterns such as sulfur-and bromine-containing (77,78).…”

Section: Capabilities For Small Molecule Analysesmentioning

confidence: 99%

Evolution of Orbitrap Mass Spectrometry Instrumentation

Eliuk

Makarov²

2015

Annual Rev. Anal. Chem.

377

329

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We discuss the evolution of Orbitrap mass spectrometry (MS) from its birth in the late 1990s to its current role as one of the most prominent techniques for MS. The Orbitrap mass analyzer is the first high-performance mass analyzer that employs trapping of ions in electrostatic fields. Tight integration with the ion injection process enables the high-resolution, mass accuracy, and sensitivity that have become essential for addressing analytical needs in numerous areas of research, as well as in routine analysis. We examine three major families of instruments (related to the LTQ Orbitrap, Q Exactive, and Orbitrap Fusion mass spectrometers) in the context of their historical development over the past ten eventful years. We discuss as well future trends and perspectives of Orbitrap MS. We illustrate the compelling potential of Orbitrap-based mass spectrometers as (ultra) high-resolution platforms, not only for high-end proteomic applications, but also for routine targeted analysis.

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Section: Capabilities For Small Molecule Analysesmentioning

confidence: 99%

Evolution of Orbitrap Mass Spectrometry Instrumentation

Eliuk

Makarov²

2015

Annual Rev. Anal. Chem.

377

329

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“…FTMS can reliably deliver mass accuracy below 1 ppm (54,(57)(58)(59)(60). This accomplishment can be aided by exploiting the use of certain ubiquitously present background ions.…”

Section: Performance Characteristics Of Ftmsmentioning

confidence: 99%

Fourier Transform Mass Spectrometry

Ščigelová

Hornshaw

Giannakopulos

et al. 2011

Molecular & Cellular Proteomics

180

137

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Mass spectrometers have been used for a long time in a variety of biological applications. Recent years, however, have witnessed a significant increase in the employment of mass spectrometers such as of time-of-flight, Fourier transform ion cyclotron resonance (FTICR) 1 , and Orbitrap, which provide accurate mass of analytes over wide mass range. The FTICR and Orbitrap analyzers outperform any other commonly used mass spectrometer with respect to the maximum mass resolution and accuracy routinely achievable even for small numbers of ions. Both these mass spectrometers share certain features, such as an image current detection system and the application of Fourier transform mathematical operations for generating mass spectra from time domain transients produced by the image current. Consequently, they are often referred to as Fourier transform-based mass spectrometers (FTMS).The combination of FTMS with ion preselection and fragmentation devices and their coupling to reversed-phase liquid chromatography (LC) represents a ubiquitous approach to both small molecule and proteomic analyses. Multidimensional LC separations have an important role to play in proteomics applications for reducing sample complexity, and complement well the high dynamic range of detection in an acquisition offered by FTMS instruments.The current article does not intend to be an exhaustive review paper on FTMS techniques; it is intended as teaching material for scientists without a physics background to assist them with understanding the mass spectrometry tools they are called to use in their everyday laboratory work. Thus, only the fundamental aspects of FTMS which are useful to a nonphysicist are introduced. The discussion then focuses on examples of where this technology can be applied and what are the benefits as well as limitations of this technology for practical proteomic applications.Need for High Resolution and Mass Accuracy-The mass accuracy is the ratio of the m/z measurement error to the true m/z, usually quoted in parts per million (ppm). The mass resolving power (resolution) is the measure of the ability to distinguish two peaks of slightly different m/z, herein understood as full width at half maximum (FWHM). Linearity of detection and very high fidelity in the determination of frequency are inherent to FTMS and allow very high resolving power, mass accuracy, and dynamic range to be achieved. But why would one need high mass accuracy and high resolution?The benefit of measuring a compound's mass with adequately high accuracy can directly determine its elemental composition. Accurate mass thus acts as a powerful "filter" useful for confirming the identity of a compound or even identification of an unknown. This is illustrated in Fig. 1 showing the fragmentation (tandem mass spectrometry (MS/MS)) spectrum of flavonoid quercetin (m/z 303). Mass deviation of less than 3 ppm for any detected fragment together with the richness of the fragmentation spectra itself enable confirming the elemental composition of the starting compound a...

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“…Thus, HPLC-ICP-MS is practically useful for the screening and determination of column recovery and the monitoring of the purification efficiency of selenometabolites owing to its sensitivity and robustness. 38) As a complementary technique to HPLC-ICP-MS, electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI)-tandem mass spectrometry (MS-MS) have been used for the identification of unknown selenometabolites in yeast, [39][40][41] plant, 42) and animal samples. 43,44) The fact that Se produces a characteristic cluster of signals that reflect its isotopic pattern ( Se, 8.73%) has facilitated the scanning of Se-containing species by MS. As ESI is a softer technique than ICP, it can provide molecular information.…”

Section: Hyphenated Techniquementioning

confidence: 99%

Selenometabolomics Explored by Speciation

Ogra

Anan

2012

Biological & Pharmaceutical Bulletin

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Selenium (Se) belongs to the same group as sulfur in the periodic table but possesses certain chemical properties characteristic of a metal. It is an essential element in animals but becomes severely toxic when the amount ingested exceeds the required level. On the other hand, Se is not essential in plants although some plants are Se hyperaccumulators. Se changes into several chemical forms when metabolized. Thus, the identification of selenometabolites would enable us to formulate a metabolic chart of Se. Recently, speciation analysis by hyphenated techniques has contributed immensely to the study of selenometabolomes, i.e., the entirety of selenometabolites. Indeed, speciation has unveiled some unique selenometabolites in biological samples. The aim of this review is to present newly identified selenometabolites in animals and plants by speciation using hyphenated techniques and to delineate the perspectives of Se biology and toxicology from the viewpoint of speciation.

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Speciation Analysis of Selenium Metabolites in Yeast-Based Food Supplements by ICPMS—Assisted Hydrophilic Interaction HPLC—Hybrid Linear Ion Trap/Orbitrap MSⁿ

Cited by 67 publications

References 48 publications

Evolution of Orbitrap Mass Spectrometry Instrumentation

Evolution of Orbitrap Mass Spectrometry Instrumentation

Fourier Transform Mass Spectrometry

Selenometabolomics Explored by Speciation

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Speciation Analysis of Selenium Metabolites in Yeast-Based Food Supplements by ICPMS—Assisted Hydrophilic Interaction HPLC—Hybrid Linear Ion Trap/Orbitrap MSn

Cited by 67 publications

References 48 publications

Evolution of Orbitrap Mass Spectrometry Instrumentation

Evolution of Orbitrap Mass Spectrometry Instrumentation

Fourier Transform Mass Spectrometry

Selenometabolomics Explored by Speciation

Contact Info

Product

Resources

About

Speciation Analysis of Selenium Metabolites in Yeast-Based Food Supplements by ICPMS—Assisted Hydrophilic Interaction HPLC—Hybrid Linear Ion Trap/Orbitrap MSⁿ