Specialized metabolites of the fungal genus Phomopsis: Structures, bioactivities and biosynthesis

Zhao, Meilu; Ge, Yichao; Zhou, Chengzeng; Liu, Xiaowan; Wu, Bin

doi:10.1016/j.fitote.2023.105523

Cited by 2 publications

(2 citation statements)

References 71 publications

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“…According to the literature investigation, [34][35][36][37] different active chemicals, such as sesquiterpenoids, steroids, isocoumarin, cytochalasins, xanthonesun, and precedented carbon skeletons, have been discovered in specialized metabolites of the fungi Phomopsis sp., and the number and variety of specialized metabolites from the fungal genus Phomopsis are still increasing. However, the identified structures in this study are quite different from the structures reported in the literature because of the fermentation conditions.…”

Section: Discussionmentioning

confidence: 99%

Secondary Metabolite Analysis of Phomopsis sp. LGT‐5 Based on the Dual Guidance of Whole‐Genome Sequencing and HPLC‐Q‐ToF‐MS/MS

Zhu

et al. 2023

Chemistry & Biodiversity

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Microorganisms produce a wealth of structurally diverse specialized metabolites with a remarkable range of biological activities. The Phomopsis sp. LGT‐5 was obtained through tissue block and repeatedly crossed methods from Tripterygium wilfordii Hook. F. The antibacterial experiments of LGT‐5 showed that it has high inhibitory activity against Staphylococcus aureus and Pseudomonas aeruginosa, and moderate inhibitory activity against Candida albicans. To research the generation of the antibacterial phenomenon of LGT‐5 and provide support for further research and application, the whole genome sequencing (WGS) of LGT‐5 was obtained by single‐molecule real‐time DNA sequencing platform Pacific Biosciences (PacBio) sequencing and Illumina paired‐end sequencing. The final assembled LGT‐5 genome is 54.79 Mb with a contig N50 of 290.07 kb; in addition, its secondary metabolites were detected through HPLC‐Q‐ToF‐MS/MS. By comparing its MS/MS data, the secondary metabolites were analyzed based on visual network maps obtained on the Global Natural Products Social Molecular Networking (GNPS). The analysis results showed that the secondary metabolites of LGT‐5 were triterpenes and various cyclic dipeptides.

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Section: Discussionmentioning

confidence: 99%

Secondary Metabolite Analysis of Phomopsis sp. LGT‐5 Based on the Dual Guidance of Whole‐Genome Sequencing and HPLC‐Q‐ToF‐MS/MS

Zhu

et al. 2023

Chemistry & Biodiversity

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“…[7,12,63] The cytochalasins are fungal toxins with cell membrane permeability that exhibit structural diversity and several biological effects, such as cytotoxic, anticancer, antimicrobial and antiparasitic activities, inhibition of acetylcholinesterase enzyme in vitro and promotion of lysosomal function. [12,[64][65][66][67][68] 5-methylmelein is a known phytotoxic compound produced by Xylariaceae species, including Biscogniauxia mediterranea. [69,70] In other studies, caprylic acid and 5-methylmelein have shown antifungal, antibacterial, and cytotoxic activity.…”

Section: Antifungal Compoundsmentioning

confidence: 99%

Cactus Endophytic Fungi and Bioprospecting for their Enzymes and Bioactive Molecules: A Systematic Review

de Menezes Souza,

de Menezes Fonseca,

Pinheiro de Souza

et al. 2023

Chemistry & Biodiversity

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Endophytic fungi are associated with plant health and represent a remarkable source of potential of enzymes and bioactive compounds, but the diversity of endophytes remains uncertain and poorly explored, especially in Cactaceae, one of the most species‐rich families adapted to growing in arid and semi‐arid regions. The aim of this study was to conduct a systematic review on the diversity and bioprospecting of endophytic fungi from Cactaceae. We analysed peer‐reviewed articles from seven databases using PRISMA guidelines. The results showed that the Cactaceae family is a source of new taxa, but the diversity of endophytic fungi of Cactaceae is little explored, mainly the diversity among tissues and by metagenomics. Bioprospecting studies have shown that these microorganisms can be used in the production of enzymes and larvicidal and antifungal compounds. Our results are relevant as a starting point for researchers to develop studies that expand the knowledge of plant mycobiota in arid and semi‐arid ecosystems, as well as comprising a remarkable source of fungal compounds with several biotechnological applications.

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Specialized metabolites of the fungal genus Phomopsis: Structures, bioactivities and biosynthesis

Cited by 2 publications

References 71 publications

Secondary Metabolite Analysis of Phomopsis sp. LGT‐5 Based on the Dual Guidance of Whole‐Genome Sequencing and HPLC‐Q‐ToF‐MS/MS

Secondary Metabolite Analysis of Phomopsis sp. LGT‐5 Based on the Dual Guidance of Whole‐Genome Sequencing and HPLC‐Q‐ToF‐MS/MS

Cactus Endophytic Fungi and Bioprospecting for their Enzymes and Bioactive Molecules: A Systematic Review

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