Speciality Grand Challenge for “Biofilms”

Beloin, Christophe; McDougald, Diane

doi:10.3389/fcimb.2021.632429

Cited by 16 publications

(14 citation statements)

References 73 publications

(46 reference statements)

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“…To eradicate MDR bacteria, another challenge must be addressed, i.e., bacterial biofilm formation, which is associated with increased resistance to several antimicrobials ( Dzianach et al, 2019 ; Beloin and McDougald, 2021 ; Rathod et al, 2021 ). In this sense, selected MDR bacteria of Enterococcus sp., Staphylococcus sp.…”

Section: Discussionmentioning

confidence: 99%

Antibacterial and antibiofilm effects of essential oil components, EDTA and HLE disinfectant solution on Enterococcus, Pseudomonas and Staphylococcus sp. multiresistant strains isolated along the meat production chain

Gómez

Manetsberger²,

Benomar³

et al. 2022

Front. Microbiol.

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The spread of multidrug resistant (MDR) bacteria and resistance genes along the food chain and the environment has become a global, but silent pandemic. To face this challenge, it is of outmost importance to develop efficient strategies to reduce potential contamination by these agents. In the present study, 30 strains of Enterococcus sp., Staphylococcus sp. and Pseudomonas sp. isolated from various surfaces throughout the meat production chain in a goat and lamb slaughterhouse were characterized as MDR bacteria harboring several antibiotic resistance genes (ARGs). The antimicrobial efficacy of natural essential oil components “EOCs” (carvacrol “CA,” cinnamaldehyde “CIN,” eugenol “EU,” geraniol “GE,” limonene “LI” and thymol “TH”), HLE disinfectant solution (3–6% H2O2; 2.2–4.4% lactic acid and 12.5–25 mM EDTA in water) and EDTA was tested against these MDR bacteria. Results showed that Minimum Inhibitory Concentrations (MIC) were compound and strain dependent. In addition, the synergistic effect of these antimicrobials was evaluated at 1/2 MIC. Here our study showed particularly promising results regarding the inhibitory effect at sub-inhibitory concentrations, which were confirmed by the analysis of bacterial growth dynamics over 72 h. Furthermore, the inhibitory effect of EOCs, HLE disinfectant solution and EDTA or their combinations was studied in developing and established biofilms of MDR bacteria obtaining variable results depending on the morphological structure of the tested strain and the phenolic character of the EOCs. Importantly, the combination of EOCs with HLE or EDTA showed particularly positive results given the effective inhibition of biofilm formation. Moreover, the synergistic combinations of EU and HLE/EDTA, TH, CA, GE, LI or CIN + EDTA/HLE caused log reductions in established biofilms of several strains (1–6 log10 CFU) depending on the species and the combination used, with Pseudomonas sp. strains being the most susceptible. Given these results, we propose novel antimicrobial formulations based on the combination of sub-inhibitory concentrations of EOCs and HLE or EDTA as a highly promising alternative to currently used approaches. This novel strategy notably shows great potential to efficiently decrease the emergence and spread of MDR bacteria and ARGs in the food chain and the environment, thus supporting the decrease of resistomes and pathogenesis in clinical and industrial areas while preserving the antibiotic therapeutic action.

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Section: Discussionmentioning

confidence: 99%

Antibacterial and antibiofilm effects of essential oil components, EDTA and HLE disinfectant solution on Enterococcus, Pseudomonas and Staphylococcus sp. multiresistant strains isolated along the meat production chain

Gómez

Manetsberger²,

Benomar³

et al. 2022

Front. Microbiol.

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“…The production of the extracellular polymeric matrix and the 3D expansion of biofilm indeed lead to heterogeneity of phenotypes due to chemical gradients within the structure. This heterogeneity is considered a hallmark of biofilms, along with a challenge to tackle to understand how specific functional properties emerge [7]. Nevertheless, a large number of methodologies still commonly used in biofilm studies provide only a global analysis of the whole biofilm and do not consider this spatial heterogeneity [8].…”

Section: A Three-dimensional Lifestylementioning

confidence: 99%

Microbial Biofilms: Structural Plasticity and Emerging Properties

Bridier

Briandet

2022

Microorganisms

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Microbial biofilms are found everywhere and can be either beneficial or detrimental, as they are involved in crucial ecological processes and in severe chronic infections. The functional properties of biofilms are closely related to their three-dimensional (3D) structure, and the ability of microorganisms to collectively and dynamically shape the community spatial organization in response to stresses in such biological edifices. A large number of works have shown a relationship between the modulation of the spatial organization and ecological interactions in biofilms in response to environmental fluctuations, as well as their emerging properties essential for nutrient cycling and bioremediation processes in natural environments. On the contrary, numerous studies have emphasized the role of structural rearrangements and matrix production in the increased tolerance of bacteria in biofilms toward antimicrobials. In these last few years, the development of innovative approaches, relying on recent technological advances in imaging, computing capacity, and other analytical tools, has led to the production of original data that have improved our understanding of this close relationship. However, it has also highlighted the need to delve deeper into the study of cell behavior in such complex communities during 3D structure development and maturation— from a single-cell to a multicellular scale— to better control or harness positive and negative impacts of biofilms. For this Special Issue, the interplay between biofilm emerging properties and their 3D spatial organization considering different models, from single bacteria to complex environmental communities, and various environments, from natural ecosystems to industrial and medical settings are addressed.

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“…Biofilms-associated infections (e.g., implant-associated infections, urethritis, vaginitis,cystic fibrosis) are generally chronic in which the treatment is quite challenging as cells are embedded in a matrix of extracellular polymeric substances (EPS), making them less susceptible to antimicrobial agents, drugs, and host immunity 3 – 5 . It is thought that the increased microbial resistance is a result of the different cellular metabolism of biofilm cells compared to planktonic cells, as well as the presence of EPS which acts as a diffusion-limiting barrier to antibiotics, thus limiting access to the deeper layers of a biofilm 6 , 7 . Hence, there is a compelling need to discover antibiotic-sparing anti-biofilm compounds to combat these biofilm-related infections.…”

Section: Introductionmentioning

confidence: 99%

“…7 CFU/mL of overnight cultures were incubated with different concentrations of caffeine (100 μM, 500 μM, 1 mM, 4 mM, 8 mM, 12 mM, 16 mM, 20 mM, 24 mM and 48 mM) in a 96-well plate (SPL Lifesciences, cat #32096) at 28 °C for 48 h. The culture was washed with 1× PBS three times for quantification to remove planktonic bacteria. The biofilm formed was then heat-fixed at 60 °C for 1 h. The formed biofilms were then stained with 0.1% crystal violet for 30 min.…”

mentioning

confidence: 99%

Anti-biofilm activity of caffeine against uropathogenic E. coli is mediated by curli biogenesis

Rathi

Gupta

Kumar

et al. 2022

Sci Rep

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Biofilms are assemblages of sessile microorganisms that form an extracellular matrix around themselves and mediate attachment to surfaces. The major component of the extracellular matrix of Uropathogenic E. coli and other Enterobacteriaceae are curli fibers, making biofilms robust and resistant to antimicrobials. It is therefore imperative to screen antibiofilm compounds that can impair biofilm formation. In the present study, we investigated the curli-dependent antibiofilm activity of caffeine against UPEC strain CFT073 and commensal strain E. coli K-12MG1655.Caffeine significantly reduced the biofilm formation of both UPEC and E. coli K-12 by 86.58% and 91.80% respectively at 48 mM caffeine as determined by Crystal Violet assay. These results were further confirmed by fluorescence microscopy and Scanning Electron Microscope (SEM). Caffeine significantly reduced the cytotoxicity and survivability of UPEC. Molecular docking analysis revealed a strong interaction between caffeine and curli regulator protein (Csg D) of E. coli. The qRT-PCR data also showed significant downregulation in the expression of CsgBA and the CsgDEFG operon at both 24 mM and 48 mM caffeine. The findings revealed that caffeine could inhibit E. coli biofilm formation by regulating curli assembly and thus may be used as an alternative therapeutic strategy for the treatment of chronic E. coli biofilm-related infections.

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Speciality Grand Challenge for “Biofilms”

Cited by 16 publications

References 73 publications

Antibacterial and antibiofilm effects of essential oil components, EDTA and HLE disinfectant solution on Enterococcus, Pseudomonas and Staphylococcus sp. multiresistant strains isolated along the meat production chain

Antibacterial and antibiofilm effects of essential oil components, EDTA and HLE disinfectant solution on Enterococcus, Pseudomonas and Staphylococcus sp. multiresistant strains isolated along the meat production chain

Microbial Biofilms: Structural Plasticity and Emerging Properties

Anti-biofilm activity of caffeine against uropathogenic E. coli is mediated by curli biogenesis

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