Special Section Guest Editorial: Multiphoton Microscopy: Technical Innovations, Biological Applications, and Clinical Diagnostics

Dong, Chongying; So, Peter T. C.; Mertz, Jérôme; Koenig, Karsten; Xu, Chris; Campagnola, Paul J.

doi:10.1117/1.jbo.18.3.031101

Cited by 3 publications

(3 citation statements)

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“…In the same time, as multi-photon imaging of tissue/cell is becoming a widely used method to study different medical diseases and conditions52, the proposed framework could represent a consistent solution for other diagnostic scenarios such as TPEF based differentiation between normal, inflammatory and neoplastic lung53, normal and cancerous gastric tissues54 or normal, benign, and cancer affected breast tissues55. The influence of the three DSIFT-BOF parameters that we have evaluated in our experiment is directly connected to the image content in terms of tissue morphology, and for this reason the presented results are mainly relevant for the addressed problem: TPEF based liver fibrosis diagnostic.…”

Section: Discussionmentioning

confidence: 99%

Experimenting Liver Fibrosis Diagnostic by Two Photon Excitation Microscopy and Bag-of-Features Image Classification

Stanciu

Xu²,

Qian

et al. 2014

Sci Rep

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The accurate staging of liver fibrosis is of paramount importance to determine the state of disease progression, therapy responses, and to optimize disease treatment strategies. Non-linear optical microscopy techniques such as two-photon excitation fluorescence (TPEF) and second harmonic generation (SHG) can image the endogenous signals of tissue structures and can be used for fibrosis assessment on non-stained tissue samples. While image analysis of collagen in SHG images was consistently addressed until now, cellular and tissue information included in TPEF images, such as inflammatory and hepatic cell damage, equally important as collagen deposition imaged by SHG, remain poorly exploited to date. We address this situation by experimenting liver fibrosis quantification and scoring using a combined approach based on TPEF liver surface imaging on a Thioacetamide-induced rat model and a gradient based Bag-of-Features (BoF) image classification strategy. We report the assessed performance results and discuss the influence of specific BoF parameters to the performance of the fibrosis scoring framework.

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Section: Discussionmentioning

confidence: 99%

Experimenting Liver Fibrosis Diagnostic by Two Photon Excitation Microscopy and Bag-of-Features Image Classification

Stanciu

Xu²,

Qian

et al. 2014

Sci Rep

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“…In a wide-field illumination geometry, 3D imaging is not possible because the beams are too wide. To achieve 3D imaging and to improve the transverse spatial resolution (21), we used an adapted HiLo imaging procedure (18,(22)(23)(24)(25)(26)(27) that relies on partial spatial coherence introduced by a chirp in the illuminating pulse. The procedure is based on Fourier filtering using a cosine intensity pattern containing a carrier spatial frequency to reject out-of-focus light (20).…”

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confidence: 99%

Optical imaging of surface chemistry and dynamics in confinement

Macias-Romero

Nahálka

Okur

et al. 2017

Science

106

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Surface chemistry and dynamics are optically imaged label-free and non-invasively in a 3D confined geometry and on sub-second time scales AbstractThe interfacial structure and dynamics of water in a microscopically confined geometry is imaged in three dimensions and on millisecond time scales. We developed a 3D wide-field second harmonic microscope that employs structured illumination. We image pH induced chemical changes on the curved and confined inner and outer surfaces of a cylindrical glass micro-capillary immersed in aqueous solution. The image contrast reports on the orientational order of interfacial water, induced by chargedipole interactions between water molecules and surface charges. The images constitute surface potential maps. Spatially resolved surface pK a,s values are determined for the silica deprotonation reaction.Values range from 2.3

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“…[9][10][11][12][13] The circular intensity difference (CID) describes the difference in absorption of left and right circularly polarized light. For isotropic media, this phenomenon can only occur in chiral molecules, which by definition have non-superimposable enantiomeric structures [14].…”

Section: Introductionmentioning

confidence: 99%

Gauge-origin independent calculations of electric-field-induced second-harmonic generation circular intensity difference using London atomic orbitals

2016

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We present the first gauge-origin independent calculations of the circular intensity difference (CID) in electric-field-induced second-harmonic generation (EFISHG), including all contributions up to the electric-quadrupole-magnetic-dipole level.A recursive, open-ended response theory framework in combination with the use of London atomic orbitals allows us to ensure gauge-origin independent results. We apply this approach to study EFISHG-CID in a collection of chiral amino acids. We demonstrate that diffuse polarizing basis functions are critical in order to obtain accurate CIDs, and that a basis set of at least aug-cc-pVTZ quality is needed in order to obtain results close to the basis-set limit. The use of London orbitals does not lead to significantly faster basis set convergence, although the improved basis set convergence allows the aug-cc-pVDZ basis set to be used with some confidence for larger molecules.

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Special Section Guest Editorial: Multiphoton Microscopy: Technical Innovations, Biological Applications, and Clinical Diagnostics

Cited by 3 publications

References 0 publications

Experimenting Liver Fibrosis Diagnostic by Two Photon Excitation Microscopy and Bag-of-Features Image Classification

Experimenting Liver Fibrosis Diagnostic by Two Photon Excitation Microscopy and Bag-of-Features Image Classification

Optical imaging of surface chemistry and dynamics in confinement

Gauge-origin independent calculations of electric-field-induced second-harmonic generation circular intensity difference using London atomic orbitals

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