Special Issue on “The Application of Metabolomics in Clinical Practice: Challenges and Opportunities”

Mussap, Michele

doi:10.3390/metabo12040296

Cited by 2 publications

(3 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Precision medicine moves toward to adopt new therapeutic approaches based on the unique, individual, genetic and phenotypic response in clinical practice [241]. The human metabolome database [242] contains nearly 221,000 entries of metabolites, which are categorized by disease, age, gender, and source (serum, urine, feces, cerebrospinal fluid, sweat) and enriched with structural data.…”

Section: Can the Gsh S-conjugates Be Considered Novel Metabolic Signa...mentioning

confidence: 99%

“…These analyses are mainly non-invasive, but the selection of the ideal GSH S-conjugates as biomarkers should take into account their clear-cut correlation with the relevant disease, stability, half-life and method(s) for samples' preparation. gathered in the field of metabolomics that these data should be exploited for potential biomarkers [241]. Screening for errors in metabolism is a characteristic example of the targeted metabolomic analysis [242].…”

Section: Can the Gsh S-conjugates Be Considered Novel Metabolic Signa...mentioning

confidence: 99%

See 1 more Smart Citation

The Key Role of GSH in Keeping the Redox Balance in Mammalian Cells: Mechanisms and Significance of GSH in Detoxification via Formation of Conjugates

Georgiou-Siafis,

Tsiftsoglou

2023

Antioxidants

View full text Add to dashboard Cite

Glutathione (GSH) is a ubiquitous tripeptide that is biosynthesized in situ at high concentrations (1–5 mM) and involved in the regulation of cellular homeostasis via multiple mechanisms. The main known action of GSH is its antioxidant capacity, which aids in maintaining the redox cycle of cells. To this end, GSH peroxidases contribute to the scavenging of various forms of ROS and RNS. A generally underestimated mechanism of action of GSH is its direct nucleophilic interaction with electrophilic compounds yielding thioether GSH S-conjugates. Many compounds, including xenobiotics (such as NAPQI, simvastatin, cisplatin, and barbital) and intrinsic compounds (such as menadione, leukotrienes, prostaglandins, and dopamine), form covalent adducts with GSH leading mainly to their detoxification. In the present article, we wish to present the key role and significance of GSH in cellular redox biology. This includes an update on the formation of GSH-S conjugates or GSH adducts with emphasis given to the mechanism of reaction, the dependence on GST (GSH S-transferase), where this conjugation occurs in tissues, and its significance. The uncovering of the GSH adducts’ formation enhances our knowledge of the human metabolome. GSH–hematin adducts were recently shown to have been formed spontaneously in multiples isomers at hemolysates, leading to structural destabilization of the endogenous toxin, hematin (free heme), which is derived from the released hemoglobin. Moreover, hemin (the form of oxidized heme) has been found to act through the Kelch-like ECH associated protein 1 (Keap1)–nuclear factor erythroid 2-related factor-2 (Nrf2) signaling pathway as an epigenetic modulator of GSH metabolism. Last but not least, the implications of the genetic defects in GSH metabolism, recorded in hemolytic syndromes, cancer and other pathologies, are presented and discussed under the framework of conceptualizing that GSH S-conjugates could be regarded as signatures of the cellular metabolism in the diseased state.

show abstract

Section: Can the Gsh S-conjugates Be Considered Novel Metabolic Signa...mentioning

confidence: 99%

Section: Can the Gsh S-conjugates Be Considered Novel Metabolic Signa...mentioning

confidence: 99%

The Key Role of GSH in Keeping the Redox Balance in Mammalian Cells: Mechanisms and Significance of GSH in Detoxification via Formation of Conjugates

Georgiou-Siafis,

Tsiftsoglou

2023

Antioxidants

View full text Add to dashboard Cite

show abstract

“…Metabolomics has exploded in the last two decades and is now routinely used in clinics, pharmaceutical companies, , the food industry, nutraceutics, environmental studies, forensic applications, among others. Indeed, a number of more focused branches have emerged under the general umbrella of metabolomics such as phytomics, exposomics, and lipidomics .…”

mentioning

confidence: 99%

MARS: A Multipurpose Software for Untargeted LC–MS-Based Metabolomics and Exposomics

Goracci,

Tiberi,

Di Bona

et al. 2024

Anal. Chem.

View full text Add to dashboard Cite

Untargeted metabolomics is a growing field, in which recent advances in high-resolution mass spectrometry coupled with liquid chromatography (LC-MS) have facilitated untargeted approaches as a result of improvements in sensitivity, mass accuracy, and resolving power. However, a very large amount of data are generated. Consequently, using computational tools is now mandatory for the in-depth analysis of untargeted metabolomics data. This article describes MetAbolomics ReSearch (MARS), an all-in-one vendor-agnostic graphical user interface-based software applying LC-MS analysis to untargeted metabolomics. All of the analytical steps are described (from instrument data conversion and processing to statistical analysis, annotation/identification, quantification, and preliminary biological interpretation), and tools developed to improve annotation accuracy (e.g., multiple adducts and in-source fragmentation detection, trends across samples, and the MS/MS validator) are highlighted. In addition, MARS allows in-house building of reference databases, to bypass the limits of freely available MS/MS spectra collections. Focusing on the flexibility of the software and its user-friendliness, which are two important features in multipurpose software, MARS could provide new perspectives in untargeted metabolomics data analysis.

show abstract

Special Issue on “The Application of Metabolomics in Clinical Practice: Challenges and Opportunities”

Abstract: This Special Issue aimed to collect studies based on clinical applications of metabolomics in human disease [...]

Cited by 2 publications

References 21 publications

The Key Role of GSH in Keeping the Redox Balance in Mammalian Cells: Mechanisms and Significance of GSH in Detoxification via Formation of Conjugates

The Key Role of GSH in Keeping the Redox Balance in Mammalian Cells: Mechanisms and Significance of GSH in Detoxification via Formation of Conjugates

MARS: A Multipurpose Software for Untargeted LC–MS-Based Metabolomics and Exposomics

Contact Info

Product

Resources

About