Spearmint R2R3‐<scp>MYB</scp> transcription factor Ms<scp>MYB</scp> negatively regulates monoterpene production and suppresses the expression of geranyl diphosphate synthase large subunit (<i>Ms<scp>GPPS</scp></i>.<i><scp>LSU</scp></i>)

Reddy, Vaishnavi Amarr; Wang, Qian; Dhar, Niha; Kumar, Nitin; Venkatesh, Prasanna Nori; Rajan, Chakravarthy; Panicker, Deepa; Sridhar, Vishweshwaran; Mao, Hui-Zhu; Sarojam, Rajani

doi:10.1111/pbi.12701

Cited by 116 publications

(75 citation statements)

References 103 publications

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“…Plants produce a large number of metabolites that are essential for ecological interactions and terpenes are natural products of the largest and structurally most diverse class ( Reddy et al., 2017 ). Terpenes play an important role in the communication between plants and the environment, between plants and animals, and between plants and plants; they can attract pollinators and seed spreaders, and they can act as defense agents against herbivores and pathogens.…”

Section: Introductionmentioning

confidence: 99%

MdMYC2 and MdERF3 Positively Co-Regulate α-Farnesene Biosynthesis in Apple

et al. 2020

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α-Farnesene, a sesquiterpene volatile compound plays an important role in plant defense and is known to be associated with insect attraction and with superficial scald of apple and pear fruits during cold storage. But the mechanism whereby transcription factors regulate apple α-farnesene biosynthesis has not been clarified. Here, we report that two transcription factors, MdMYC2 and MdERF3 regulated α-farnesene biosynthesis in apple fruit. Dual-luciferase assays and Y1H assays indicated that MdMYC2 and MdERF3 effectively trans-activated the MdAFS promoter. EMSAs showed that MdERF3 directly binds the DRE motif in the MdAFS promoter. Subsequently, overexpression of MdMYC2 and MdERF3 in apple calli markedly activated the transcript levels of MdHMGR2 and MdAFS . Furthermore, transient overexpression of MdMYC2 and MdERF3 in apple fruit significantly increased MdAFS expression and hence, α-farnesene production. These results indicate that MdMYC2 and MdERF3 are positive regulators of α-farnesene biosynthesis and have important value in genetic engineering of α-farnesene production.

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Section: Introductionmentioning

confidence: 99%

MdMYC2 and MdERF3 Positively Co-Regulate α-Farnesene Biosynthesis in Apple

et al. 2020

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“…For instance, PtMYB14 is a putative regulator of an isoprenoid-oriented response that contributes to the accumulation of sesquiterpene in conifers [49]. MsMYB combines with the promoter of geranyl diphosphate synthase and suppresses its activity to regulate monoterpene production negatively [24]. AtMYB21 and AtMYB24 contribute to the production of sesquiterpenes [42].…”

Section: Discussionmentioning

confidence: 99%

“…It was reported that SmMYB36 and SmMYB9b in salvia could positively regulate the biosynthesis of tanshinone [22,23]. Only MsMYB has been investigated to be a negative regulator of monoterpene biosynthesis by suppressing the expression of geranyl diphosphate synthase [24]. The roles of 2R-MYB genes and TFs in the terpenoid pathways of O. fragrans remain to be elucidated.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification, Classification, and Expression Profiling Reveals R2R3-MYB Transcription Factors Related to Monoterpenoid Biosynthesis in Osmanthus fragrans

Yue

Ding

et al. 2020

Genes

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Osmanthus fragrans is widely grown for the purpose of urban greening and the pleasant aroma emitted from its flowers. The floral scent is determined by several monoterpenoid volatiles, such as linalool and its oxides, which are a few of the most common volatiles and the main components of the essential oils in most sweet osmanthus cultivars. In addition, the relative contents of cis- and trans-linalool oxide (furan) may affect the aromas and quality of the essential oils. MYB proteins represent the largest family of transcription factors in plants and participate in regulating secondary metabolites. Several cis-elements, especially AC-rich regions, are known to be bound by 2R-MYBs and could be found in the promoter of the enzyme genes in the terpenoid metabolic pathway. However, there has to date been no investigation into the 2R-MYB family genes involved in regulating terpenoid biosynthesis in O. fragrans. Here, 243 non-redundant 2R-MYB proteins were grouped into 33 clusters based on the phylogeny and exon-intron distribution. These genes were unevenly distributed on 23 chromosomes. Ka/Ks analysis showed that the major mode of 2R-MYB gene evolution was purifying selection. Expression analysis indicated that 2R-MYB genes in O. fragrans exhibited varied expression patterns. A total of 35 OfMYBs representing the highest per kilobase per million mapped reads in the flower were selected for quantitative real-time PCR analysis. The correlation analysis between the expression level and the contents of fragrant compounds at different flowering stages suggested that OfMYB19/20 exhibited remarkably positive correlation with the accumulation of cis-linalool oxides. OfMYB51/65/88/121/137/144 showed significantly negative correlations with one or more linalool oxides. Characterization of these proteins revealed that OfMYB19 and OfMYB137 were localized in the nuclei, but did not show transcriptional activation in the yeast system, which suggested that they may be bound to other transcription factors to exert regulatory functions. These findings provide useful information for further functional investigation of the 2R-MYBs and offer a foundation for clarifying the 2R-MYB transcription factors involved in the molecular mechanism of the regulation of monoterpenoid biosynthesis in Osmanthus fragrans.

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“…The members of this family extensively participated in terpenoids biosynthesis, not only in triterpenoids, but also other terpenoids. For example, spearmint (Mentha spicata) MsMYB binds to cis-elements of MsGPPS.LSU and suppresses monoterpene biosynthesis [35]. Artemisia annua AaMYB1 acts as activator in diterpene metabolism (artemisinin, AN) [36].…”

Section: Introductionmentioning

confidence: 99%

MYB transcription factor CiMYB42 regulates limonoids biosynthesis in citrus

Pan

Liu

et al. 2020

Preprint

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Backgrounds Limonoids, a major bioactive components, are produced by triterpenoids metabolic pathway. So far, the detailed biochemical reactions regarding to limonoid biosynthesis and their molecular regulation remain elusive. The identification of transcription factors that regulate limonoids biosynthetic pathways is not only necessary for understanding the regulatory mechanisms but also as a tool for manipulating biosynthetic genes for biotechnological applications.Results In this study, CiMYB42 transcription factor was isolated and identified. Multiple alignment analysis and phylogenetic analysis demonstrated that CiMYB42 is a typical R2R3MYB transcription factor and its amino acid is similar to that of AtMYB42 . The limonoids content was higher in the citrus sinensis and citrus grandis than other species. The diverse accumulation patterns also showed in different leaf developmental stages. Expression of CiMYB42 was significantly related to limonoids content and the expression of CiOSC in some of citrus varieties. CiMYB42 transgenic sweet orange resulted significantly change on limonoids contents. Noticeably, CiMYB42 -RNAi induced dwarf phenotype and mainly decreased nomilin accumulation. Overexpressing CiMYB42 mainly increased limonin content. Yeast one hybrid assay results indicated that CiMYB42 exclusively bind to the promoter of CiOSC. In brief, CiMYB42 involved in the limonoids biosynthesis through binding the promoter of CiOSC in citrus.Conclusions These results indicated that CiMYB42 is an important transcription activator involved in limonoids biosynthesis by regulating the expression of CiOSC . This is the first report elucidating the role of transcription factor in citrus limonoids biosynthesis. Our contributions will provide a reference to understanding regulatory mechanisms of R2R3MYB TFs in the triterpenoids biosynthetic pathway.

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Spearmint R2R3‐MYB transcription factor MsMYB negatively regulates monoterpene production and suppresses the expression of geranyl diphosphate synthase large subunit (MsGPPS.LSU)

Cited by 116 publications

References 103 publications

MdMYC2 and MdERF3 Positively Co-Regulate α-Farnesene Biosynthesis in Apple

MdMYC2 and MdERF3 Positively Co-Regulate α-Farnesene Biosynthesis in Apple

Genome-Wide Identification, Classification, and Expression Profiling Reveals R2R3-MYB Transcription Factors Related to Monoterpenoid Biosynthesis in Osmanthus fragrans

MYB transcription factor CiMYB42 regulates limonoids biosynthesis in citrus

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